UniProtKB - O00584 (RNT2_HUMAN)
Ribonuclease T2
RNASET2
Functioni
Catalytic activityi
- RNA + H(2)O = an (RNA fragment)-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment).PROSITE-ProRule annotation EC:4.6.1.19
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 65 | PROSITE-ProRule annotation | 1 | |
Active sitei | 114 | By similarity | 1 | |
Active sitei | 118 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- endoribonuclease activity Source: GO_Central
- lyase activity Source: UniProtKB-KW
- ribonuclease activity Source: UniProtKB
- ribonuclease T2 activity Source: UniProtKB-EC
- RNA binding Source: InterPro
GO - Biological processi
- innate immune response Source: UniProtKB-KW
- neutrophil degranulation Source: Reactome
- RNA catabolic process Source: UniProtKB
Keywordsi
Molecular function | Endonuclease, Hydrolase, Lyase, Nuclease |
Biological process | Immunity, Innate immunity |
Enzyme and pathway databases
PathwayCommonsi | O00584 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease T2 (EC:4.6.1.19PROSITE-ProRule annotation)Alternative name(s): Ribonuclease 6 |
Gene namesi | Name:RNASET2 Synonyms:RNASE6PL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21686, RNASET2 |
MIMi | 612944, gene |
neXtProti | NX_O00584 |
VEuPathDBi | HostDB:ENSG00000026297.15 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen 1 Publication
Lysosome
- Lysosome lumen 1 Publication
Extracellular region or secreted
- Secreted 2 Publications
Mitochondrion
- Mitochondrion intermembrane space 3 Publications
Note: Full-length RNASET2 is found in the endoplasmic reticulum while smaller RNASET2 proteolytic products are found in the lysosome fraction.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: GO_Central
- extracellular space Source: UniProtKB
Lysosome
- azurophil granule lumen Source: Reactome
- lysosomal lumen Source: UniProtKB-SubCell
- lysosome Source: UniProtKB
Mitochondrion
- mitochondrial intermembrane space Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulum, Lysosome, Mitochondrion, SecretedPathology & Biotechi
Involvement in diseasei
Leukoencephalopathy, cystic, without megalencephaly (LCWM)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_063596 | 184 | C → R in LCWM; the loss of a disulfide bond may affect protein folding and stability; the protein is retained in the endoplasmic reticulum and the mitochondria while lysosomal localization is dirupted. 3 PublicationsCorresponds to variant dbSNP:rs121918137EnsemblClinVar. | 1 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 65 | H → Y: Abolishes the effect on degradation of mitochondrion-associated cytosolic rRNAs. 1 Publication | 1 | |
Mutagenesisi | 118 | H → Y: Abolishes the effect on degradation of mitochondrion-associated cytosolic rRNAs. 1 Publication | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 8635 |
MalaCardsi | RNASET2 |
MIMi | 612951, phenotype |
OpenTargetsi | ENSG00000026297 |
Orphaneti | 85136, Cystic leukoencephalopathy without megalencephaly |
PharmGKBi | PA128394541 |
Miscellaneous databases
Pharosi | O00584, Tbio |
Genetic variation databases
BioMutai | RNASET2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000030987 | 25 – 256 | Ribonuclease T2Add BLAST | 232 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 48 ↔ 55 | 1 Publication | ||
Disulfide bondi | 75 ↔ 121 | 1 Publication | ||
Glycosylationi | 76 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 106 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 184 ↔ 241 | 1 Publication | ||
Disulfide bondi | 202 ↔ 213 | 1 Publication | ||
Glycosylationi | 212 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | O00584 |
jPOSTi | O00584 |
MassIVEi | O00584 |
MaxQBi | O00584 |
PaxDbi | O00584 |
PeptideAtlasi | O00584 |
PRIDEi | O00584 |
ProteomicsDBi | 47985 [O00584-1] 47986 [O00584-2] |
PTM databases
GlyConnecti | 1719, 7 N-Linked glycans (2 sites) |
GlyGeni | O00584, 4 sites, 2 N-linked glycans (1 site), 1 O-linked glycan (1 site) |
iPTMneti | O00584 |
PhosphoSitePlusi | O00584 |
SwissPalmi | O00584 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000026297, Expressed in endometrium and 243 other tissues |
ExpressionAtlasi | O00584, baseline and differential |
Genevisiblei | O00584, HS |
Organism-specific databases
HPAi | ENSG00000026297, Tissue enhanced (blood) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 114188, 14 interactors |
IntActi | O00584, 7 interactors |
STRINGi | 9606.ENSP00000426455 |
Miscellaneous databases
RNActi | O00584, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O00584 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1642, Eukaryota |
GeneTreei | ENSGT00640000091563 |
HOGENOMi | CLU_2037289_0_0_1 |
InParanoidi | O00584 |
OrthoDBi | 994722at2759 |
PhylomeDBi | O00584 |
TreeFami | TF315063 |
Family and domain databases
CDDi | cd01061, RNase_T2_euk, 1 hit |
Gene3Di | 3.90.730.10, 1 hit |
InterProi | View protein in InterPro IPR033697, Ribonuclease_T2_eukaryotic IPR001568, RNase_T2-like IPR036430, RNase_T2-like_sf IPR018188, RNase_T2_His_AS_1 IPR033130, RNase_T2_His_AS_2 |
PANTHERi | PTHR11240, PTHR11240, 1 hit |
Pfami | View protein in Pfam PF00445, Ribonuclease_T2, 1 hit |
SUPFAMi | SSF55895, SSF55895, 1 hit |
PROSITEi | View protein in PROSITE PS00530, RNASE_T2_1, 1 hit PS00531, RNASE_T2_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPAALRGAL LGCLCLALLC LGGADKRLRD NHEWKKLIMV QHWPETVCEK
60 70 80 90 100
IQNDCRDPPD YWTIHGLWPD KSEGCNRSWP FNLEEIKDLL PEMRAYWPDV
110 120 130 140 150
IHSFPNRSRF WKHEWEKHGT CAAQVDALNS QKKYFGRSLE LYRELDLNSV
160 170 180 190 200
LLKLGIKPSI NYYQVADFKD ALARVYGVIP KIQCLPPSQD EEVQTIGQIE
210 220 230 240 250
LCLTKQDQQL QNCTEPGEQP SPKQEVWLAN GAAESRGLRV CEDGPVFYPP
PKKTKH
The sequence of this isoform differs from the canonical sequence as follows:
88-121: DLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTC → KNWMEITDSSLPSPSTLPIINIFYSVLHLLQLMN
122-256: Missing.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RHI9 | D6RHI9_HUMAN | Ribonuclease T2 | RNASET2 | 253 | Annotation score: | ||
A0A087WZM2 | A0A087WZM2_HUMAN | Ribonuclease T2 | RNASET2 | 306 | Annotation score: | ||
A0A087WWI1 | A0A087WWI1_HUMAN | Ribonuclease T2 | RNASET2 | 238 | Annotation score: | ||
D6REQ6 | D6REQ6_HUMAN | Ribonuclease T2 | RNASET2 hCG_15018 | 218 | Annotation score: | ||
J3QQ64 | J3QQ64_HUMAN | Ribonuclease T2 | RNASET2 | 71 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_063596 | 184 | C → R in LCWM; the loss of a disulfide bond may affect protein folding and stability; the protein is retained in the endoplasmic reticulum and the mitochondria while lysosomal localization is dirupted. 3 PublicationsCorresponds to variant dbSNP:rs121918137EnsemblClinVar. | 1 | |
Natural variantiVAR_013004 | 236 | R → W. Corresponds to variant dbSNP:rs11159EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008405 | 88 – 121 | DLLPE…KHGTC → KNWMEITDSSLPSPSTLPII NIFYSVLHLLQLMN in isoform 2. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_008406 | 122 – 256 | Missing in isoform 2. 1 PublicationAdd BLAST | 135 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U85625 mRNA Translation: AAC51363.2 AJ419865 mRNA Translation: CAD12030.1 AJ419866 mRNA Translation: CAD12031.1 AK315467 mRNA Translation: BAG37854.1 AL133458 Genomic DNA No translation available. AL159163 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW47512.1 CH471051 Genomic DNA Translation: EAW47513.1 CH471051 Genomic DNA Translation: EAW47514.1 BC001660 mRNA Translation: AAH01660.1 BC001819 mRNA Translation: AAH01819.1 BC039713 mRNA Translation: AAH39713.1 BC051912 mRNA Translation: AAH51912.1 |
CCDSi | CCDS5295.1 [O00584-1] |
PIRi | S78046 |
RefSeqi | NP_003721.2, NM_003730.4 [O00584-1] |
Genome annotation databases
Ensembli | ENST00000421787; ENSP00000390833; ENSG00000026297 [O00584-2] ENST00000476238; ENSP00000422846; ENSG00000026297 [O00584-1] ENST00000508775; ENSP00000426455; ENSG00000026297 [O00584-1] |
GeneIDi | 8635 |
KEGGi | hsa:8635 |
UCSCi | uc003qve.4, human [O00584-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U85625 mRNA Translation: AAC51363.2 AJ419865 mRNA Translation: CAD12030.1 AJ419866 mRNA Translation: CAD12031.1 AK315467 mRNA Translation: BAG37854.1 AL133458 Genomic DNA No translation available. AL159163 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW47512.1 CH471051 Genomic DNA Translation: EAW47513.1 CH471051 Genomic DNA Translation: EAW47514.1 BC001660 mRNA Translation: AAH01660.1 BC001819 mRNA Translation: AAH01819.1 BC039713 mRNA Translation: AAH39713.1 BC051912 mRNA Translation: AAH51912.1 |
CCDSi | CCDS5295.1 [O00584-1] |
PIRi | S78046 |
RefSeqi | NP_003721.2, NM_003730.4 [O00584-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3T0O | X-ray | 1.59 | A | 25-256 | [»] | |
SMRi | O00584 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114188, 14 interactors |
IntActi | O00584, 7 interactors |
STRINGi | 9606.ENSP00000426455 |
PTM databases
GlyConnecti | 1719, 7 N-Linked glycans (2 sites) |
GlyGeni | O00584, 4 sites, 2 N-linked glycans (1 site), 1 O-linked glycan (1 site) |
iPTMneti | O00584 |
PhosphoSitePlusi | O00584 |
SwissPalmi | O00584 |
Genetic variation databases
BioMutai | RNASET2 |
Proteomic databases
EPDi | O00584 |
jPOSTi | O00584 |
MassIVEi | O00584 |
MaxQBi | O00584 |
PaxDbi | O00584 |
PeptideAtlasi | O00584 |
PRIDEi | O00584 |
ProteomicsDBi | 47985 [O00584-1] 47986 [O00584-2] |
Protocols and materials databases
Antibodypediai | 33528, 189 antibodies |
Genome annotation databases
Ensembli | ENST00000421787; ENSP00000390833; ENSG00000026297 [O00584-2] ENST00000476238; ENSP00000422846; ENSG00000026297 [O00584-1] ENST00000508775; ENSP00000426455; ENSG00000026297 [O00584-1] |
GeneIDi | 8635 |
KEGGi | hsa:8635 |
UCSCi | uc003qve.4, human [O00584-1] |
Organism-specific databases
CTDi | 8635 |
DisGeNETi | 8635 |
GeneCardsi | RNASET2 |
HGNCi | HGNC:21686, RNASET2 |
HPAi | ENSG00000026297, Tissue enhanced (blood) |
MalaCardsi | RNASET2 |
MIMi | 612944, gene 612951, phenotype |
neXtProti | NX_O00584 |
OpenTargetsi | ENSG00000026297 |
Orphaneti | 85136, Cystic leukoencephalopathy without megalencephaly |
PharmGKBi | PA128394541 |
VEuPathDBi | HostDB:ENSG00000026297.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1642, Eukaryota |
GeneTreei | ENSGT00640000091563 |
HOGENOMi | CLU_2037289_0_0_1 |
InParanoidi | O00584 |
OrthoDBi | 994722at2759 |
PhylomeDBi | O00584 |
TreeFami | TF315063 |
Enzyme and pathway databases
PathwayCommonsi | O00584 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 8635, 7 hits in 987 CRISPR screens |
ChiTaRSi | RNASET2, human |
GeneWikii | RNASET2 |
GenomeRNAii | 8635 |
Pharosi | O00584, Tbio |
PROi | PR:O00584 |
RNActi | O00584, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000026297, Expressed in endometrium and 243 other tissues |
ExpressionAtlasi | O00584, baseline and differential |
Genevisiblei | O00584, HS |
Family and domain databases
CDDi | cd01061, RNase_T2_euk, 1 hit |
Gene3Di | 3.90.730.10, 1 hit |
InterProi | View protein in InterPro IPR033697, Ribonuclease_T2_eukaryotic IPR001568, RNase_T2-like IPR036430, RNase_T2-like_sf IPR018188, RNase_T2_His_AS_1 IPR033130, RNase_T2_His_AS_2 |
PANTHERi | PTHR11240, PTHR11240, 1 hit |
Pfami | View protein in Pfam PF00445, Ribonuclease_T2, 1 hit |
SUPFAMi | SSF55895, SSF55895, 1 hit |
PROSITEi | View protein in PROSITE PS00530, RNASE_T2_1, 1 hit PS00531, RNASE_T2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNT2_HUMAN | |
Accessioni | O00584Primary (citable) accession number: O00584 Secondary accession number(s): B2RDA7 Q9BZ47 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2002 |
Last sequence update: | November 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families