Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase 25

Gene

STK25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Activity regulationi

Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-174, possibly as a consequence of stabilization of dimer formation. The C-terminal non-catalytic region inhibits the kinase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei140Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase kinase kinase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • protein kinase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO00506
SIGNORiO00506

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 25 (EC:2.7.11.1)
Alternative name(s):
Ste20-like kinase
Sterile 20/oxidant stress-response kinase 1
Short name:
SOK-1
Short name:
Ste20/oxidant stress response kinase 1
Gene namesi
Name:STK25
Synonyms:SOK1, YSK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115694.14
HGNCiHGNC:11404 STK25
MIMi602255 gene
neXtProtiNX_O00506

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49K → R: Loss of kinase activity and autophosphorylation. 1 Publication1
Mutagenesisi158D → A: Loss of kinase activity. 1 Publication1

Organism-specific databases

DisGeNETi10494
OpenTargetsiENSG00000115694
PharmGKBiPA36211

Chemistry databases

ChEMBLiCHEMBL5552

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867131 – 426Serine/threonine-protein kinase 25Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174Phosphothreonine; by autocatalysis1 Publication1
Modified residuei278PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO00506
MaxQBiO00506
PaxDbiO00506
PeptideAtlasiO00506
PRIDEiO00506
ProteomicsDBi47947

PTM databases

iPTMnetiO00506
PhosphoSitePlusiO00506
SwissPalmiO00506

Expressioni

Tissue specificityi

Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.

Gene expression databases

BgeeiENSG00000115694 Expressed in 209 organ(s), highest expression level in gastrocnemius
CleanExiHS_STK25
ExpressionAtlasiO00506 baseline and differential
GenevisibleiO00506 HS

Organism-specific databases

HPAiHPA047147

Interactioni

Subunit structurei

Homodimer. Interacts with CTTNBP2NL.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115757, 53 interactors
DIPiDIP-34269N
IntActiO00506, 44 interactors
MINTiO00506
STRINGi9606.ENSP00000325748

Chemistry databases

BindingDBiO00506

Structurei

Secondary structure

1426
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO00506
SMRiO00506
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 270Protein kinasePROSITE-ProRule annotationAdd BLAST251

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0201 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00920000149000
HOGENOMiHOG000234203
HOVERGENiHBG108518
InParanoidiO00506
KOiK08838
OMAiFSHNRNH
OrthoDBiEOG091G0F24
PhylomeDBiO00506
TreeFamiTF354217

Family and domain databases

CDDicd06642 STKc_STK25, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR035060 STK_STK25
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 12 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: O00506-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAHLRGFANQ HSRVDPEELF TKLDRIGKGS FGEVYKGIDN HTKEVVAIKI
60 70 80 90 100
IDLEEAEDEI EDIQQEITVL SQCDSPYITR YFGSYLKSTK LWIIMEYLGG
110 120 130 140 150
GSALDLLKPG PLEETYIATI LREILKGLDY LHSERKIHRD IKAANVLLSE
160 170 180 190 200
QGDVKLADFG VAGQLTDTQI KRNTFVGTPF WMAPEVIKQS AYDFKADIWS
210 220 230 240 250
LGITAIELAK GEPPNSDLHP MRVLFLIPKN SPPTLEGQHS KPFKEFVEAC
260 270 280 290 300
LNKDPRFRPT AKELLKHKFI TRYTKKTSFL TELIDRYKRW KSEGHGEESS
310 320 330 340 350
SEDSDIDGEA EDGEQGPIWT FPPTIRPSPH SKLHKGTALH SSQKPAEPVK
360 370 380 390 400
RQPRSQCLST LVRPVFGELK EKHKQSGGSV GALEELENAF SLAEESCPGI
410 420
SDKLMVHLVE RVQRFSHNRN HLTSTR
Length:426
Mass (Da):48,112
Last modified:July 1, 1997 - v1
Checksum:i183CE5700FCEA716
GO
Isoform 2 (identifier: O00506-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-87: Missing.

Note: No experimental confirmation available.
Show »
Length:349
Mass (Da):39,266
Checksum:iF1BC727F54CB32A4
GO
Isoform 3 (identifier: O00506-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.
Show »
Length:332
Mass (Da):37,297
Checksum:iB4D6A56543812A3C
GO

Computationally mapped potential isoform sequencesi

There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JFA1C9JFA1_HUMAN
Serine/threonine-protein kinase 25
STK25
89Annotation score:
C9JN58C9JN58_HUMAN
Serine/threonine-protein kinase 25
STK25
115Annotation score:
E7EM58E7EM58_HUMAN
Serine/threonine-protein kinase 25
STK25
151Annotation score:
C9J232C9J232_HUMAN
Serine/threonine-protein kinase 25
STK25
177Annotation score:
C9JDH9C9JDH9_HUMAN
Serine/threonine-protein kinase 25
STK25
148Annotation score:
C9JCC0C9JCC0_HUMAN
Serine/threonine-protein kinase 25
STK25
163Annotation score:
C9J6L2C9J6L2_HUMAN
Serine/threonine-protein kinase 25
STK25
161Annotation score:
H7C279H7C279_HUMAN
Serine/threonine-protein kinase 25
STK25
247Annotation score:
C9JJV0C9JJV0_HUMAN
Serine/threonine-protein kinase 25
STK25
79Annotation score:
C9K0M6C9K0M6_HUMAN
Serine/threonine-protein kinase 25
STK25
48Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347 – 348EP → DA in CAA67700 (PubMed:8887545).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05167464Q → H. Corresponds to variant dbSNP:rs34341643Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546831 – 94Missing in isoform 3. 1 PublicationAdd BLAST94
Alternative sequenceiVSP_05439711 – 87Missing in isoform 2. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99325 mRNA Translation: CAA67700.1
D63780 mRNA Translation: BAA20420.1
AK291808 mRNA Translation: BAF84497.1
AK291947 mRNA Translation: BAF84636.1
AK315966 mRNA Translation: BAH14337.1
BT019961 mRNA Translation: AAV38764.1
AC110299 Genomic DNA Translation: AAY14683.1
DQ093965 Genomic DNA Translation: AAY88740.1
CH471063 Genomic DNA Translation: EAW71265.1
BC007852 mRNA Translation: AAH07852.1
BC091505 mRNA Translation: AAH91505.1
CCDSiCCDS2549.1 [O00506-1]
CCDS63199.1 [O00506-3]
CCDS63200.1 [O00506-2]
PIRiS71886
RefSeqiNP_001258906.1, NM_001271977.1 [O00506-1]
NP_001258907.1, NM_001271978.1 [O00506-1]
NP_001258908.1, NM_001271979.1 [O00506-2]
NP_001258909.1, NM_001271980.1 [O00506-2]
NP_001269234.1, NM_001282305.1 [O00506-3]
NP_001269236.1, NM_001282307.1 [O00506-3]
NP_001269237.1, NM_001282308.1 [O00506-3]
NP_006365.2, NM_006374.4 [O00506-1]
UniGeneiHs.516807

Genome annotation databases

EnsembliENST00000316586; ENSP00000325748; ENSG00000115694 [O00506-1]
ENST00000401869; ENSP00000385687; ENSG00000115694 [O00506-1]
ENST00000403346; ENSP00000384162; ENSG00000115694 [O00506-1]
ENST00000405585; ENSP00000385541; ENSG00000115694 [O00506-2]
ENST00000405883; ENSP00000384444; ENSG00000115694 [O00506-2]
ENST00000535007; ENSP00000446008; ENSG00000115694 [O00506-3]
ENST00000543554; ENSP00000444886; ENSG00000115694 [O00506-3]
GeneIDi10494
KEGGihsa:10494
UCSCiuc002wbm.5 human [O00506-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99325 mRNA Translation: CAA67700.1
D63780 mRNA Translation: BAA20420.1
AK291808 mRNA Translation: BAF84497.1
AK291947 mRNA Translation: BAF84636.1
AK315966 mRNA Translation: BAH14337.1
BT019961 mRNA Translation: AAV38764.1
AC110299 Genomic DNA Translation: AAY14683.1
DQ093965 Genomic DNA Translation: AAY88740.1
CH471063 Genomic DNA Translation: EAW71265.1
BC007852 mRNA Translation: AAH07852.1
BC091505 mRNA Translation: AAH91505.1
CCDSiCCDS2549.1 [O00506-1]
CCDS63199.1 [O00506-3]
CCDS63200.1 [O00506-2]
PIRiS71886
RefSeqiNP_001258906.1, NM_001271977.1 [O00506-1]
NP_001258907.1, NM_001271978.1 [O00506-1]
NP_001258908.1, NM_001271979.1 [O00506-2]
NP_001258909.1, NM_001271980.1 [O00506-2]
NP_001269234.1, NM_001282305.1 [O00506-3]
NP_001269236.1, NM_001282307.1 [O00506-3]
NP_001269237.1, NM_001282308.1 [O00506-3]
NP_006365.2, NM_006374.4 [O00506-1]
UniGeneiHs.516807

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XIKX-ray1.97A1-293[»]
3W8HX-ray2.43B355-426[»]
4NZWX-ray3.58B1-293[»]
ProteinModelPortaliO00506
SMRiO00506
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115757, 53 interactors
DIPiDIP-34269N
IntActiO00506, 44 interactors
MINTiO00506
STRINGi9606.ENSP00000325748

Chemistry databases

BindingDBiO00506
ChEMBLiCHEMBL5552

PTM databases

iPTMnetiO00506
PhosphoSitePlusiO00506
SwissPalmiO00506

Proteomic databases

EPDiO00506
MaxQBiO00506
PaxDbiO00506
PeptideAtlasiO00506
PRIDEiO00506
ProteomicsDBi47947

Protocols and materials databases

DNASUi10494
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316586; ENSP00000325748; ENSG00000115694 [O00506-1]
ENST00000401869; ENSP00000385687; ENSG00000115694 [O00506-1]
ENST00000403346; ENSP00000384162; ENSG00000115694 [O00506-1]
ENST00000405585; ENSP00000385541; ENSG00000115694 [O00506-2]
ENST00000405883; ENSP00000384444; ENSG00000115694 [O00506-2]
ENST00000535007; ENSP00000446008; ENSG00000115694 [O00506-3]
ENST00000543554; ENSP00000444886; ENSG00000115694 [O00506-3]
GeneIDi10494
KEGGihsa:10494
UCSCiuc002wbm.5 human [O00506-1]

Organism-specific databases

CTDi10494
DisGeNETi10494
EuPathDBiHostDB:ENSG00000115694.14
GeneCardsiSTK25
HGNCiHGNC:11404 STK25
HPAiHPA047147
MIMi602255 gene
neXtProtiNX_O00506
OpenTargetsiENSG00000115694
PharmGKBiPA36211
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0201 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00920000149000
HOGENOMiHOG000234203
HOVERGENiHBG108518
InParanoidiO00506
KOiK08838
OMAiFSHNRNH
OrthoDBiEOG091G0F24
PhylomeDBiO00506
TreeFamiTF354217

Enzyme and pathway databases

SignaLinkiO00506
SIGNORiO00506

Miscellaneous databases

ChiTaRSiSTK25 human
GeneWikiiSTK25
GenomeRNAii10494
PROiPR:O00506
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115694 Expressed in 209 organ(s), highest expression level in gastrocnemius
CleanExiHS_STK25
ExpressionAtlasiO00506 baseline and differential
GenevisibleiO00506 HS

Family and domain databases

CDDicd06642 STKc_STK25, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR035060 STK_STK25
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTK25_HUMAN
AccessioniPrimary (citable) accession number: O00506
Secondary accession number(s): A8K6Z3
, A8K7D2, B7Z9K1, Q15522, Q5BJF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 188 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again