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Protein

26S proteasome non-ATPase regulatory subunit 14

Gene

PSMD14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi115Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi126Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • endopeptidase activator activity Source: UniProtKB
  • Lys63-specific deubiquitinase activity Source: Reactome
  • metal ion binding Source: UniProtKB-KW
  • metallopeptidase activity Source: GO_Central
  • proteasome binding Source: UniProtKB
  • thiol-dependent ubiquitin-specific protease activity Source: GO_Central
  • thiol-dependent ubiquitinyl hydrolase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-5689901 Metalloprotease DUBs
R-HSA-6798695 Neutrophil degranulation
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 14 (EC:3.4.19.-)
Alternative name(s):
26S proteasome regulatory subunit RPN11
26S proteasome-associated PAD1 homolog 1
Gene namesi
Name:PSMD14
Synonyms:POH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115233.11
HGNCiHGNC:16889 PSMD14
MIMi607173 gene
neXtProtiNX_O00487

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113 – 115HSH → ASA: Abolishes ubiquitin thioesterase activity, leading to prevent maintenance of JMJD2A/KDM4A on chromatin. 1 Publication3

Organism-specific databases

DisGeNETi10213
OpenTargetsiENSG00000115233
PharmGKBiPA134957776

Chemistry databases

ChEMBLiCHEMBL2007629

Polymorphism and mutation databases

BioMutaiPSMD14

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139521 – 31026S proteasome non-ATPase regulatory subunit 14Add BLAST310

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei266PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO00487
MaxQBiO00487
PaxDbiO00487
PeptideAtlasiO00487
PRIDEiO00487
ProteomicsDBi47932
TopDownProteomicsiO00487

2D gel databases

OGPiO00487
REPRODUCTION-2DPAGEiIPI00024821

PTM databases

iPTMnetiO00487
PhosphoSitePlusiO00487
SwissPalmiO00487

Expressioni

Tissue specificityi

Widely expressed. Highest levels in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000115233 Expressed in 236 organ(s), highest expression level in deltoid
CleanExiHS_PSMD14
ExpressionAtlasiO00487 baseline and differential
GenevisibleiO00487 HS

Organism-specific databases

HPAiHPA002114
HPA003828

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD4, a base containing 6 ATPases and few additional components (PubMed:27428775, PubMed:27342858). Within the complex, PSMD4 interacts with subunit PSMD7 through their respective MPN domain. Interacts with TXNL1 (PubMed:19349277).4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115508, 118 interactors
CORUMiO00487
IntActiO00487, 70 interactors
MINTiO00487
STRINGi9606.ENSP00000386541

Chemistry databases

BindingDBiO00487

Structurei

3D structure databases

ProteinModelPortaliO00487
SMRiO00487
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 166MPNPROSITE-ProRule annotationAdd BLAST136

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi113 – 126JAMM motifPROSITE-ProRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the peptidase M67A family. PSMD14 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1555 Eukaryota
COG1310 LUCA
GeneTreeiENSGT00730000111116
HOGENOMiHOG000183690
HOVERGENiHBG053742
InParanoidiO00487
KOiK03030
OMAiDCFRLIN
OrthoDBiEOG091G0CMC
PhylomeDBiO00487
TreeFamiTF105748

Family and domain databases

InterProiView protein in InterPro
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR035299 PSMD14
IPR024969 Rpn11/EIF3F_C
PANTHERiPTHR10410:SF13 PTHR10410:SF13, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF13012 MitMem_reg, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O00487-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP
60 70 80 90 100
MEVMGLMLGE FVDDYTVRVI DVFAMPQSGT GVSVEAVDPV FQAKMLDMLK
110 120 130 140 150
QTGRPEMVVG WYHSHPGFGC WLSGVDINTQ QSFEALSERA VAVVVDPIQS
160 170 180 190 200
VKGKVVIDAF RLINANMMVL GHEPRQTTSN LGHLNKPSIQ ALIHGLNRHY
210 220 230 240 250
YSITINYRKN ELEQKMLLNL HKKSWMEGLT LQDYSEHCKH NESVVKEMLE
260 270 280 290 300
LAKNYNKAVE EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL
310
AAMLDTVVFK
Length:310
Mass (Da):34,577
Last modified:July 1, 1997 - v1
Checksum:i18ACE876C7682039
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JW37C9JW37_HUMAN
26S proteasome non-ATPase regulator...
PSMD14
84Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86782 mRNA Translation: AAC51866.1
AK055128 mRNA Translation: BAG51474.1
CH471058 Genomic DNA Translation: EAX11370.1
BC066336 mRNA Translation: AAH66336.1
CCDSiCCDS46437.1
RefSeqiNP_005796.1, NM_005805.5
UniGeneiHs.740477

Genome annotation databases

EnsembliENST00000409682; ENSP00000386541; ENSG00000115233
GeneIDi10213
KEGGihsa:10213
UCSCiuc002ubu.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86782 mRNA Translation: AAC51866.1
AK055128 mRNA Translation: BAG51474.1
CH471058 Genomic DNA Translation: EAX11370.1
BC066336 mRNA Translation: AAH66336.1
CCDSiCCDS46437.1
RefSeqiNP_005796.1, NM_005805.5
UniGeneiHs.740477

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50V1-310[»]
5GJRelectron microscopy3.509/V1-310[»]
5L4Kelectron microscopy4.50V1-310[»]
5LN3electron microscopy6.80V1-310[»]
5M32electron microscopy3.80q1-310[»]
5T0Celectron microscopy3.80Ac/Bc1-310[»]
5T0Gelectron microscopy4.40c2-310[»]
5T0Helectron microscopy6.80c2-310[»]
5T0Ielectron microscopy8.00c2-310[»]
5T0Jelectron microscopy8.00c2-310[»]
5VFPelectron microscopy4.20c24-310[»]
5VFQelectron microscopy4.20c24-310[»]
5VFRelectron microscopy4.90c24-310[»]
5VFSelectron microscopy3.60c2-310[»]
5VFTelectron microscopy7.00c24-310[»]
5VFUelectron microscopy5.80c24-310[»]
5VGZelectron microscopy3.70c24-310[»]
5VHFelectron microscopy5.70c24-310[»]
5VHHelectron microscopy6.10c24-310[»]
5VHIelectron microscopy6.80c24-310[»]
5VHSelectron microscopy8.80c24-310[»]
ProteinModelPortaliO00487
SMRiO00487
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115508, 118 interactors
CORUMiO00487
IntActiO00487, 70 interactors
MINTiO00487
STRINGi9606.ENSP00000386541

Chemistry databases

BindingDBiO00487
ChEMBLiCHEMBL2007629

PTM databases

iPTMnetiO00487
PhosphoSitePlusiO00487
SwissPalmiO00487

Polymorphism and mutation databases

BioMutaiPSMD14

2D gel databases

OGPiO00487
REPRODUCTION-2DPAGEiIPI00024821

Proteomic databases

EPDiO00487
MaxQBiO00487
PaxDbiO00487
PeptideAtlasiO00487
PRIDEiO00487
ProteomicsDBi47932
TopDownProteomicsiO00487

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409682; ENSP00000386541; ENSG00000115233
GeneIDi10213
KEGGihsa:10213
UCSCiuc002ubu.4 human

Organism-specific databases

CTDi10213
DisGeNETi10213
EuPathDBiHostDB:ENSG00000115233.11
GeneCardsiPSMD14
HGNCiHGNC:16889 PSMD14
HPAiHPA002114
HPA003828
MIMi607173 gene
neXtProtiNX_O00487
OpenTargetsiENSG00000115233
PharmGKBiPA134957776
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1555 Eukaryota
COG1310 LUCA
GeneTreeiENSGT00730000111116
HOGENOMiHOG000183690
HOVERGENiHBG053742
InParanoidiO00487
KOiK03030
OMAiDCFRLIN
OrthoDBiEOG091G0CMC
PhylomeDBiO00487
TreeFamiTF105748

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-5689901 Metalloprotease DUBs
R-HSA-6798695 Neutrophil degranulation
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiPSMD14 human
GeneWikiiPSMD14
GenomeRNAii10213
PROiPR:O00487
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115233 Expressed in 236 organ(s), highest expression level in deltoid
CleanExiHS_PSMD14
ExpressionAtlasiO00487 baseline and differential
GenevisibleiO00487 HS

Family and domain databases

InterProiView protein in InterPro
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR035299 PSMD14
IPR024969 Rpn11/EIF3F_C
PANTHERiPTHR10410:SF13 PTHR10410:SF13, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF13012 MitMem_reg, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSDE_HUMAN
AccessioniPrimary (citable) accession number: O00487
Secondary accession number(s): B3KNW2, O00176
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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