UniProtKB - O00445 (SYT5_HUMAN)
Synaptotagmin-5
SYT5
Functioni
May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).
By similarityCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 138 | Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 139 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 139 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 145 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 197 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 197 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 198 | Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 199 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 199 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 199 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 202 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 205 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 205 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 270 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 276 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 330 | Calcium 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 332 | Calcium 4PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: UniProtKB
- clathrin binding Source: GO_Central
- phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
- phosphatidylserine binding Source: GO_Central
- protein heterodimerization activity Source: Ensembl
- SNARE binding Source: GO_Central
- syntaxin binding Source: GO_Central
GO - Biological processi
- calcium-ion regulated exocytosis Source: GO_Central
- calcium ion-regulated exocytosis of neurotransmitter Source: GO_Central
- cellular response to calcium ion Source: GO_Central
- chemical synaptic transmission Source: ProtInc
- regulation of calcium ion-dependent exocytosis Source: GO_Central
- regulation of dopamine secretion Source: GO_Central
- synaptic vesicle endocytosis Source: GO_Central
- vesicle-mediated transport Source: GO_Central
Keywordsi
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | O00445 |
Reactomei | R-HSA-422356, Regulation of insulin secretion |
SignaLinki | O00445 |
Names & Taxonomyi
Protein namesi | Recommended name: Synaptotagmin-5Alternative name(s): Synaptotagmin V Short name: SytV |
Gene namesi | Name:SYT5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11513, SYT5 |
MIMi | 600782, gene |
neXtProti | NX_O00445 |
VEuPathDBi | HostDB:ENSG00000129990 |
Subcellular locationi
Endosome
- Recycling endosome membrane By similarity; Single-pass membrane protein By similarity
Other locations
- synaptic vesicle membrane By similarity; Single-pass membrane protein By similarity
Note: In mast cells, localizes to the endocytic recycling compartment.By similarity
Endosome
- recycling endosome membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- axon Source: GO_Central
- dense core granule Source: GO_Central
- exocytic vesicle Source: GO_Central
- integral component of neuronal dense core vesicle membrane Source: Ensembl
- neuronal cell body Source: Ensembl
- perinuclear region of cytoplasm Source: Ensembl
- proximal neuron projection Source: Ensembl
- synaptic vesicle membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | VesicularSequence analysisAdd BLAST | 24 | |
Transmembranei | 25 – 45 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 46 – 386 | CytoplasmicSequence analysisAdd BLAST | 341 |
Keywords - Cellular componenti
Cell junction, Cytoplasmic vesicle, Endosome, Membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 202 | S → G: Does not affect Ca(2+) affinity as measured by isothermal titration calorimetry of the mutant protein. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6861 |
OpenTargetsi | ENSG00000129990 |
PharmGKBi | PA36294 |
Miscellaneous databases
Pharosi | O00445, Tbio |
Genetic variation databases
BioMutai | SYT5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000183951 | 1 – 386 | Synaptotagmin-5Add BLAST | 386 |
Proteomic databases
jPOSTi | O00445 |
MassIVEi | O00445 |
MaxQBi | O00445 |
PaxDbi | O00445 |
PeptideAtlasi | O00445 |
PRIDEi | O00445 |
ProteomicsDBi | 47896 [O00445-1] 6480 |
PTM databases
iPTMneti | O00445 |
PhosphoSitePlusi | O00445 |
Expressioni
Gene expression databases
Bgeei | ENSG00000129990, Expressed in frontal cortex and 147 other tissues |
ExpressionAtlasi | O00445, baseline and differential |
Genevisiblei | O00445, HS |
Organism-specific databases
HPAi | ENSG00000129990, Group enriched (brain, pituitary gland) |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with both alpha- and beta-tubulin (By similarity).
By similarityGO - Molecular functioni
- clathrin binding Source: GO_Central
- protein heterodimerization activity Source: Ensembl
- SNARE binding Source: GO_Central
- syntaxin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 112725, 11 interactors |
ELMi | O00445 |
IntActi | O00445, 8 interactors |
MINTi | O00445 |
STRINGi | 9606.ENSP00000346265 |
Miscellaneous databases
RNActi | O00445, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O00445 |
SMRi | O00445 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 227 | C2 1PROSITE-ProRule annotationAdd BLAST | 120 | |
Domaini | 239 – 372 | C2 2PROSITE-ProRule annotationAdd BLAST | 134 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 23 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 17 | Pro residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1028, Eukaryota |
GeneTreei | ENSGT00940000161816 |
HOGENOMi | CLU_023008_0_1_1 |
InParanoidi | O00445 |
OMAi | CLCRKRC |
PhylomeDBi | O00445 |
TreeFami | TF315600 |
Family and domain databases
Gene3Di | 2.60.40.150, 2 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR001565, Synaptotagmin IPR028689, SYT5 |
PANTHERi | PTHR10024:SF282, PTHR10024:SF282, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 2 hits |
PRINTSi | PR00360, C2DOMAIN PR00399, SYNAPTOTAGMN |
SMARTi | View protein in SMART SM00239, C2, 2 hits |
SUPFAMi | SSF49562, SSF49562, 2 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFPEPPTPGP PSPDTPPDSS RISHGPVPPW ALATIVLVSG LLIFSCCFCL
60 70 80 90 100
YRKSCRRRTG KKSQAQAQVH LQEVKGLGQS YIDKVQPEVE ELEPAPSGPG
110 120 130 140 150
QQVADKHELG RLQYSLDYDF QSGQLLVGIL QAMGLAALDL GGSSDPYVRV
160 170 180 190 200
YLLPDKRRRY ETKVHRQTLN PHFGETFAFK VPYVELGGRV LVMAVYDFDR
210 220 230 240 250
FSRNDAIGEV RVPMSSVDLG RPVQAWRELQ AAPREEQEKL GDICFSLRYV
260 270 280 290 300
PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN
310 320 330 340 350
TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAAG
360 370 380
GAGLRHWADM LANPRRPIAQ WHSLRPPDRV RLLPAP
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EJC4 | K7EJC4_HUMAN | Synaptotagmin-5 | SYT5 | 152 | Annotation score: | ||
K7EQ59 | K7EQ59_HUMAN | Synaptotagmin-5 | SYT5 | 154 | Annotation score: | ||
K7EJ95 | K7EJ95_HUMAN | Synaptotagmin-5 | SYT5 | 49 | Annotation score: | ||
K7EJS7 | K7EJS7_HUMAN | Synaptotagmin-5 | SYT5 | 67 | Annotation score: | ||
M0QYJ7 | M0QYJ7_HUMAN | Synaptotagmin-5 | SYT5 | 22 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | H → R in BAG54160 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052240 | 4 | E → D. Corresponds to variant dbSNP:rs2301279Ensembl. | 1 | |
Natural variantiVAR_034528 | 111 | R → Q2 PublicationsCorresponds to variant dbSNP:rs11542503Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057177 | 1 – 84 | MFPEP…SYIDK → MTRGGCPVSARLFAQRPHGS WSSLLCVGLKGRLGWEWGTR VLECLCRKDSRTPPPCSRSP QPRGLHRPTRLPTPVASATA Q in isoform 2. 1 PublicationAdd BLAST | 84 | |
Alternative sequenceiVSP_057178 | 237 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X96783 Genomic DNA Translation: CAA65579.1 AK125182 mRNA Translation: BAG54160.1 AK295337 mRNA Translation: BAH12036.1 AC010327 Genomic DNA No translation available. BC046157 mRNA Translation: AAH46157.1 |
CCDSi | CCDS12919.1 [O00445-1] CCDS74455.1 [O00445-2] |
RefSeqi | NP_001284703.1, NM_001297774.1 [O00445-2] NP_003171.2, NM_003180.2 [O00445-1] XP_006723402.1, XM_006723339.3 [O00445-1] XP_016882664.1, XM_017027175.1 [O00445-1] XP_016882665.1, XM_017027176.1 |
Genome annotation databases
Ensembli | ENST00000354308.8; ENSP00000346265.2; ENSG00000129990.15 ENST00000537500.5; ENSP00000442896.1; ENSG00000129990.15 ENST00000590851.5; ENSP00000465576.1; ENSG00000129990.15 [O00445-2] |
GeneIDi | 6861 |
KEGGi | hsa:6861 |
MANE-Selecti | ENST00000354308.8; ENSP00000346265.2; NM_003180.3; NP_003171.2 |
UCSCi | uc002qjm.2, human [O00445-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X96783 Genomic DNA Translation: CAA65579.1 AK125182 mRNA Translation: BAG54160.1 AK295337 mRNA Translation: BAH12036.1 AC010327 Genomic DNA No translation available. BC046157 mRNA Translation: AAH46157.1 |
CCDSi | CCDS12919.1 [O00445-1] CCDS74455.1 [O00445-2] |
RefSeqi | NP_001284703.1, NM_001297774.1 [O00445-2] NP_003171.2, NM_003180.2 [O00445-1] XP_006723402.1, XM_006723339.3 [O00445-1] XP_016882664.1, XM_017027175.1 [O00445-1] XP_016882665.1, XM_017027176.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5H4Y | X-ray | 1.90 | A | 102-242 | [»] | |
5H4Z | X-ray | 3.01 | A/B | 102-242 | [»] | |
AlphaFoldDBi | O00445 | |||||
SMRi | O00445 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112725, 11 interactors |
ELMi | O00445 |
IntActi | O00445, 8 interactors |
MINTi | O00445 |
STRINGi | 9606.ENSP00000346265 |
PTM databases
iPTMneti | O00445 |
PhosphoSitePlusi | O00445 |
Genetic variation databases
BioMutai | SYT5 |
Proteomic databases
jPOSTi | O00445 |
MassIVEi | O00445 |
MaxQBi | O00445 |
PaxDbi | O00445 |
PeptideAtlasi | O00445 |
PRIDEi | O00445 |
ProteomicsDBi | 47896 [O00445-1] 6480 |
Protocols and materials databases
Antibodypediai | 2420, 213 antibodies from 32 providers |
DNASUi | 6861 |
Genome annotation databases
Ensembli | ENST00000354308.8; ENSP00000346265.2; ENSG00000129990.15 ENST00000537500.5; ENSP00000442896.1; ENSG00000129990.15 ENST00000590851.5; ENSP00000465576.1; ENSG00000129990.15 [O00445-2] |
GeneIDi | 6861 |
KEGGi | hsa:6861 |
MANE-Selecti | ENST00000354308.8; ENSP00000346265.2; NM_003180.3; NP_003171.2 |
UCSCi | uc002qjm.2, human [O00445-1] |
Organism-specific databases
CTDi | 6861 |
DisGeNETi | 6861 |
GeneCardsi | SYT5 |
HGNCi | HGNC:11513, SYT5 |
HPAi | ENSG00000129990, Group enriched (brain, pituitary gland) |
MIMi | 600782, gene |
neXtProti | NX_O00445 |
OpenTargetsi | ENSG00000129990 |
PharmGKBi | PA36294 |
VEuPathDBi | HostDB:ENSG00000129990 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota |
GeneTreei | ENSGT00940000161816 |
HOGENOMi | CLU_023008_0_1_1 |
InParanoidi | O00445 |
OMAi | CLCRKRC |
PhylomeDBi | O00445 |
TreeFami | TF315600 |
Enzyme and pathway databases
PathwayCommonsi | O00445 |
Reactomei | R-HSA-422356, Regulation of insulin secretion |
SignaLinki | O00445 |
Miscellaneous databases
BioGRID-ORCSi | 6861, 29 hits in 1075 CRISPR screens |
GeneWikii | SYT5 |
GenomeRNAii | 6861 |
Pharosi | O00445, Tbio |
PROi | PR:O00445 |
RNActi | O00445, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000129990, Expressed in frontal cortex and 147 other tissues |
ExpressionAtlasi | O00445, baseline and differential |
Genevisiblei | O00445, HS |
Family and domain databases
Gene3Di | 2.60.40.150, 2 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR001565, Synaptotagmin IPR028689, SYT5 |
PANTHERi | PTHR10024:SF282, PTHR10024:SF282, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 2 hits |
PRINTSi | PR00360, C2DOMAIN PR00399, SYNAPTOTAGMN |
SMARTi | View protein in SMART SM00239, C2, 2 hits |
SUPFAMi | SSF49562, SSF49562, 2 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SYT5_HUMAN | |
Accessioni | O00445Primary (citable) accession number: O00445 Secondary accession number(s): B3KWJ8, B7Z300, Q86X72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 11, 2003 | |
Last modified: | May 25, 2022 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families