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Protein

DNA-directed RNA polymerase, mitochondrial

Gene

POLRMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei922By similarity1
Active sitei991By similarity1
Active sitei1151By similarity1

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB
  • mitochondrial promoter sequence-specific DNA binding Source: GO_Central
  • RNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

ReactomeiR-HSA-163282 Mitochondrial transcription initiation
R-HSA-2151201 Transcriptional activation of mitochondrial biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
Short name:
MtRPOL
Gene namesi
Name:POLRMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000099821.13
HGNCiHGNC:9200 POLRMT
MIMi601778 gene
neXtProtiNX_O00411

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi5442
OpenTargetsiENSG00000099821
PharmGKBiPA33522

Chemistry databases

ChEMBLiCHEMBL3120041

Polymorphism and mutation databases

BioMutaiPOLRMT

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41MitochondrionSequence analysisAdd BLAST41
ChainiPRO_000003106842 – 1230DNA-directed RNA polymerase, mitochondrialAdd BLAST1189

Proteomic databases

EPDiO00411
MaxQBiO00411
PaxDbiO00411
PeptideAtlasiO00411
PRIDEiO00411
ProteomicsDBi47875

PTM databases

iPTMnetiO00411
PhosphoSitePlusiO00411

Expressioni

Gene expression databases

BgeeiENSG00000099821 Expressed in 192 organ(s), highest expression level in left testis
CleanExiHS_POLRMT
ExpressionAtlasiO00411 baseline and differential
GenevisibleiO00411 HS

Organism-specific databases

HPAiHPA006366

Interactioni

Subunit structurei

Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner (PubMed:23283301). Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT (PubMed:29445193). Interacts with TFB1M and TFB2M, leading to the stimulation of transcription (PubMed:12068295). Interacts with TEFM (PubMed:21278163).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MRPL12P528154EBI-355145,EBI-358272

Protein-protein interaction databases

BioGridi111438, 41 interactors
DIPiDIP-56412N
IntActiO00411, 21 interactors
MINTiO00411
STRINGi9606.ENSP00000215591

Structurei

Secondary structure

11230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO00411
SMRiO00411
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni802 – 1230Mediates interaction with TEFM1 PublicationAdd BLAST429

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1038 Eukaryota
COG5108 LUCA
GeneTreeiENSGT00390000008060
HOGENOMiHOG000044061
HOVERGENiHBG078730
InParanoidiO00411
KOiK10908
OMAiDWFTECA
OrthoDBiEOG091G00YJ
PhylomeDBiO00411
TreeFamiTF105700

Family and domain databases

Gene3Di1.10.1320.10, 1 hit
1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR002092 DNA-dir_Rpol_phage-type
IPR037159 RNA_POL_N_sf
IPR029262 RPOL_N
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR10102 PTHR10102, 1 hit
PfamiView protein in Pfam
PF00940 RNA_pol, 1 hit
PF14700 RPOL_N, 1 hit
SMARTiView protein in SMART
SM01311 RPOL_N, 1 hit
PROSITEiView protein in PROSITE
PS00900 RNA_POL_PHAGE_1, 1 hit
PS00489 RNA_POL_PHAGE_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O00411-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD
60 70 80 90 100
QDRRKDWGHV ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS
110 120 130 140 150
LQPPRKVQMG AKDATPVPCG RWAKILEKDK RTQQMRMQRL KAKLQMPFQS
160 170 180 190 200
GEFKALTRRL QVEPRLLSKQ MAGCLEDCTR QAPESPWEEQ LARLLQEAPG
210 220 230 240 250
KLSLDVEQAP SGQHSQAQLS GQQQRLLAFF KCCLLTDQLP LAHHLLVVHH
260 270 280 290 300
GQRQKRKLLT LDMYNAVMLG WARQGAFKEL VYVLFMVKDA GLTPDLLSYA
310 320 330 340 350
AALQCMGRQD QDAGTIERCL EQMSQEGLKL QALFTAVLLS EEDRATVLKA
360 370 380 390 400
VHKVKPTFSL PPQLPPPVNT SKLLRDVYAK DGRVSYPKLH LPLKTLQCLF
410 420 430 440 450
EKQLHMELAS RVCVVSVEKP TLPSKEVKHA RKTLKTLRDQ WEKALCRALR
460 470 480 490 500
ETKNRLEREV YEGRFSLYPF LCLLDEREVV RMLLQVLQAL PAQGESFTTL
510 520 530 540 550
ARELSARTFS RHVVQRQRVS GQVQALQNHY RKYLCLLASD AEVPEPCLPR
560 570 580 590 600
QYWEELGAPE ALREQPWPLP VQMELGKLLA EMLVQATQMP CSLDKPHRSS
610 620 630 640 650
RLVPVLYHVY SFRNVQQIGI LKPHPAYVQL LEKAAEPTLT FEAVDVPMLC
660 670 680 690 700
PPLPWTSPHS GAFLLSPTKL MRTVEGATQH QELLETCPPT ALHGALDALT
710 720 730 740 750
QLGNCAWRVN GRVLDLVLQL FQAKGCPQLG VPAPPSEAPQ PPEAHLPHSA
760 770 780 790 800
APARKAELRR ELAHCQKVAR EMHSLRAEAL YRLSLAQHLR DRVFWLPHNM
810 820 830 840 850
DFRGRTYPCP PHFNHLGSDV ARALLEFAQG RPLGPHGLDW LKIHLVNLTG
860 870 880 890 900
LKKREPLRKR LAFAEEVMDD ILDSADQPLT GRKWWMGAEE PWQTLACCME
910 920 930 940 950
VANAVRASDP AAYVSHLPVH QDGSCNGLQH YAALGRDSVG AASVNLEPSD
960 970 980 990 1000
VPQDVYSGVA AQVEVFRRQD AQRGMRVAQV LEGFITRKVV KQTVMTVVYG
1010 1020 1030 1040 1050
VTRYGGRLQI EKRLRELSDF PQEFVWEASH YLVRQVFKSL QEMFSGTRAI
1060 1070 1080 1090 1100
QHWLTESARL ISHMGSVVEW VTPLGVPVIQ PYRLDSKVKQ IGGGIQSITY
1110 1120 1130 1140 1150
THNGDISRKP NTRKQKNGFP PNFIHSLDSS HMMLTALHCY RKGLTFVSVH
1160 1170 1180 1190 1200
DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE
1210 1220 1230
ASQLKETLQA VPKPGAFDLE QVKRSTYFFS
Length:1,230
Mass (Da):138,620
Last modified:July 19, 2004 - v2
Checksum:i47AFBF2E0884AEFA
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EMH3K7EMH3_HUMAN
DNA-directed RNA polymerase, mitoch...
POLRMT
347Annotation score:
K7ESD1K7ESD1_HUMAN
DNA-directed RNA polymerase, mitoch...
POLRMT
127Annotation score:
R4GN79R4GN79_HUMAN
DNA-directed RNA polymerase, mitoch...
POLRMT
56Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti399L → F in AAB58255 (PubMed:9097968).Curated1
Sequence conflicti983G → S in AAB58255 (PubMed:9097968).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019427555E → A. Corresponds to variant dbSNP:rs2238549Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75370 mRNA Translation: AAB58255.1
AC004449 Genomic DNA Translation: AAC06147.1
CCDSiCCDS12036.1
RefSeqiNP_005026.3, NM_005035.3
UniGeneiHs.254113

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821
GeneIDi5442
KEGGihsa:5442
UCSCiuc002lpf.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75370 mRNA Translation: AAB58255.1
AC004449 Genomic DNA Translation: AAC06147.1
CCDSiCCDS12036.1
RefSeqiNP_005026.3, NM_005035.3
UniGeneiHs.254113

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
4BOCX-ray2.65A151-1230[»]
5OLAX-ray3.90E/F151-1230[»]
6ERPX-ray4.50A/B105-1230[»]
6ERQX-ray4.50A/B105-1230[»]
ProteinModelPortaliO00411
SMRiO00411
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111438, 41 interactors
DIPiDIP-56412N
IntActiO00411, 21 interactors
MINTiO00411
STRINGi9606.ENSP00000215591

Chemistry databases

ChEMBLiCHEMBL3120041

PTM databases

iPTMnetiO00411
PhosphoSitePlusiO00411

Polymorphism and mutation databases

BioMutaiPOLRMT

Proteomic databases

EPDiO00411
MaxQBiO00411
PaxDbiO00411
PeptideAtlasiO00411
PRIDEiO00411
ProteomicsDBi47875

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821
GeneIDi5442
KEGGihsa:5442
UCSCiuc002lpf.2 human

Organism-specific databases

CTDi5442
DisGeNETi5442
EuPathDBiHostDB:ENSG00000099821.13
GeneCardsiPOLRMT
H-InvDBiHIX0027511
HIX0059707
HGNCiHGNC:9200 POLRMT
HPAiHPA006366
MIMi601778 gene
neXtProtiNX_O00411
OpenTargetsiENSG00000099821
PharmGKBiPA33522
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1038 Eukaryota
COG5108 LUCA
GeneTreeiENSGT00390000008060
HOGENOMiHOG000044061
HOVERGENiHBG078730
InParanoidiO00411
KOiK10908
OMAiDWFTECA
OrthoDBiEOG091G00YJ
PhylomeDBiO00411
TreeFamiTF105700

Enzyme and pathway databases

ReactomeiR-HSA-163282 Mitochondrial transcription initiation
R-HSA-2151201 Transcriptional activation of mitochondrial biogenesis

Miscellaneous databases

GeneWikiiPOLRMT
GenomeRNAii5442
PROiPR:O00411
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000099821 Expressed in 192 organ(s), highest expression level in left testis
CleanExiHS_POLRMT
ExpressionAtlasiO00411 baseline and differential
GenevisibleiO00411 HS

Family and domain databases

Gene3Di1.10.1320.10, 1 hit
1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR002092 DNA-dir_Rpol_phage-type
IPR037159 RNA_POL_N_sf
IPR029262 RPOL_N
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR10102 PTHR10102, 1 hit
PfamiView protein in Pfam
PF00940 RNA_pol, 1 hit
PF14700 RPOL_N, 1 hit
SMARTiView protein in SMART
SM01311 RPOL_N, 1 hit
PROSITEiView protein in PROSITE
PS00900 RNA_POL_PHAGE_1, 1 hit
PS00489 RNA_POL_PHAGE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPOM_HUMAN
AccessioniPrimary (citable) accession number: O00411
Secondary accession number(s): O60370
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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