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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

WASL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis (By similarity).By similarity4 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • GTPase regulator activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processCell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-203641 NOSTRIN mediated eNOS trafficking
R-HSA-373753 Nephrin family interactions
R-HSA-3928662 EPHB-mediated forward signaling
R-HSA-418885 DCC mediated attractive signaling
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiO00401
SIGNORiO00401

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:WASL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000106299.7
HGNCiHGNC:12735 WASL
MIMi605056 gene
neXtProtiNX_O00401

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8976
OpenTargetsiENSG00000106299
PharmGKBiPA37346

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001890002 – 505Neural Wiskott-Aldrich syndrome proteinAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei242Phosphoserine; by TNK21 Publication1
Modified residuei256Phosphotyrosine; by FAK1 and TNK2Combined sources1 Publication1
Modified residuei307Omega-N-methylarginineCombined sources1
Modified residuei484PhosphoserineCombined sources1
Modified residuei485PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO00401
MaxQBiO00401
PaxDbiO00401
PeptideAtlasiO00401
PRIDEiO00401
ProteomicsDBi47867

PTM databases

iPTMnetiO00401
PhosphoSitePlusiO00401

Expressioni

Gene expression databases

BgeeiENSG00000106299
CleanExiHS_WASL
GenevisibleiO00401 HS

Organism-specific databases

HPAiCAB005399
HPA005750

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with NOSTRIN. Interacts with Shigella flexneri protein IcsA. The interaction with IcsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli effector protein EspF(U). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U). Binds to TNK2. Interacts with SNX33.By similarity8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114466, 71 interactors
CORUMiO00401
DIPiDIP-29042N
ELMiO00401
IntActiO00401, 62 interactors
MINTiO00401
STRINGi9606.ENSP00000223023

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni219 – 221Combined sources3
Helixi225 – 227Combined sources3
Helixi232 – 239Combined sources8
Helixi243 – 246Combined sources4
Turni249 – 251Combined sources3
Helixi252 – 262Combined sources11
Helixi265 – 269Combined sources5
Helixi435 – 443Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FF3X-ray2.00C451-465[»]
2LNHNMR-A207-270[»]
2VCPX-ray3.20D/E392-484[»]
4CC2X-ray1.55B/D346-357[»]
4CC7X-ray1.97B/D/F/H/J/L/N346-357[»]
ProteinModelPortaliO00401
SMRiO00401
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00401

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 141WH1PROSITE-ProRule annotationAdd BLAST108
Domaini203 – 216CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini405 – 422WH2 1PROSITE-ProRule annotationAdd BLAST18
Domaini433 – 450WH2 2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi277 – 392Pro-richAdd BLAST116
Compositional biasi485 – 505Asp-richAdd BLAST21

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671 Eukaryota
ENOG4111J0D LUCA
GeneTreeiENSGT00730000110895
HOGENOMiHOG000143378
HOVERGENiHBG000222
InParanoidiO00401
KOiK05747
OMAiKCQTLNT
OrthoDBiEOG091G0VCD
PhylomeDBiO00401
TreeFamiTF316736

Family and domain databases

CDDicd00132 CRIB, 1 hit
cd01205 EVH1_WASP-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.90.810.10, 2 hits
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR030214 N-WASP
IPR011993 PH-like_dom_sf
IPR011026 WASP_C
IPR033927 WASPfam_EVH1
IPR000697 WH1/EVH1_dom
IPR003124 WH2_dom
PANTHERiPTHR23202:SF39 PTHR23202:SF39, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00568 WH1, 1 hit
PF02205 WH2, 2 hits
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SM00461 WH1, 1 hit
SM00246 WH2, 2 hits
SUPFAMiSSF47912 SSF47912, 2 hits
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS50229 WH1, 1 hit
PS51082 WH2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA
60 70 80 90 100
ADRNCMWSKK CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY
110 120 130 140 150
NSPRGYFHTF AGDTCQVALN FANEEEAKKF RKAVTDLLGR RQRKSEKRRD
160 170 180 190 200
PPNGPNLPMA TVDIKNPEIT TNRFYGPQVN NISHTKEKKK GKAKKKRLTK
210 220 230 240 250
ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG ISEAQLKDRE
260 270 280 290 300
TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHNSGPP
310 320 330 340 350
PPPARGRGAP PPPPSRAPTA APPPPPPSRP SVAVPPPPPN RMYPPPPPAL
360 370 380 390 400
PSSAPSGPPP PPPSVLGVGP VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP
410 420 430 440 450
TTAGNKAALL DQIREGAQLK KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV
460 470 480 490 500
ADGQESTPPT PAPTSGIVGA LMEVMQKRSK AIHSSDEDED EDDEEDFEDD

DEWED
Length:505
Mass (Da):54,827
Last modified:February 1, 2005 - v2
Checksum:iB5895C6055E23049
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76Y → H in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti201A → G in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti223F → S in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti242S → L in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti248D → E in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti252S → L in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti296N → S in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti333A → E in BAA20128 (PubMed:9322739).Curated1
Sequence conflicti351P → L in CAL26602 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88460 mRNA Translation: BAA20128.1
AM295156 mRNA Translation: CAL26602.1
AC006333 Genomic DNA Translation: AAQ96857.1
BC052955 mRNA Translation: AAH52955.1
CCDSiCCDS34743.1
RefSeqiNP_003932.3, NM_003941.3
UniGeneiHs.143728
Hs.731519
Hs.83916

Genome annotation databases

EnsembliENST00000223023; ENSP00000223023; ENSG00000106299
GeneIDi8976
KEGGihsa:8976
UCSCiuc003vkz.5 human

Similar proteinsi

Entry informationi

Entry nameiWASL_HUMAN
AccessioniPrimary (citable) accession number: O00401
Secondary accession number(s): A1JUI9, Q7Z746
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 2005
Last modified: June 20, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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