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Protein

Sulfhydryl oxidase 1

Gene

QSOX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being involved in growth regulation.6 Publications

Miscellaneous

'Quiescin Q6' means that it was the sixth clone to be found at a higher level of expression in quiescent fibroblasts.

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.1 Publication

Cofactori

FAD2 PublicationsNote: Binds 1 FAD per subunit.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei70Nucleophile1 Publication1
Active sitei73Nucleophile1 Publication1
Binding sitei401FAD1 Publication1
Binding sitei408FAD1 Publication1
Binding sitei412FAD1 Publication1
Binding sitei451FAD1 Publication1
Binding sitei455FAD1 Publication1
Binding sitei500FAD1 Publication1
Binding sitei503FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi478 – 485FAD1 Publication8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.8.3.2 2681
ReactomeiR-HSA-114608 Platelet degranulation
R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-6798695 Neutrophil degranulation
R-HSA-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfhydryl oxidase 1 (EC:1.8.3.2)
Short name:
hQSOX
Alternative name(s):
Quiescin Q6
Gene namesi
Name:QSOX1
Synonyms:QSCN6
ORF Names:UNQ2520/PRO6013
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000116260.16
HGNCiHGNC:9756 QSOX1
MIMi603120 gene
neXtProtiNX_O00391

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei710 – 730HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70C → S: Reduces activity by 93%. 1 Publication1
Mutagenesisi73C → S: Reduces activity by 93%. 1 Publication1
Mutagenesisi449C → S: Reduces activity by 96%. 1 Publication1
Mutagenesisi452C → S: Loss of activity. 1 Publication1
Mutagenesisi509C → S: No effect. Reduces activity by 70%; when associated with S-512. 1 Publication1
Mutagenesisi512C → S: Reduces activity by 40%. Reduces activity by 70%; when associated with S-509. 1 Publication1

Organism-specific databases

DisGeNETi5768
OpenTargetsiENSG00000116260
PharmGKBiPA162400559

Polymorphism and mutation databases

BioMutaiQSOX1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000024953330 – 747Sulfhydryl oxidase 1Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 73Redox-active1 Publication
Glycosylationi130N-linked (GlcNAc...) (complex) asparagine2 Publications1
Glycosylationi243N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi393 ↔ 405PROSITE-ProRule annotation1 Publication
Modified residuei426Phosphoserine; by FAM20C1 Publication1
Disulfide bondi449 ↔ 452PROSITE-ProRule annotation1 Publication
Disulfide bondi509 ↔ 512PROSITE-ProRule annotation1 Publication
Glycosylationi575N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO00391
MaxQBiO00391
PaxDbiO00391
PeptideAtlasiO00391
PRIDEiO00391
ProteomicsDBi47862
47863 [O00391-2]

PTM databases

GlyConnecti1774
iPTMnetiO00391
PhosphoSitePlusiO00391

Expressioni

Tissue specificityi

Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very weakly in brain and kidney.2 Publications

Inductioni

Induced in quiescent cells just as fibroblasts begin to leave the proliferative cycle and enter quiescence.3 Publications

Gene expression databases

BgeeiENSG00000116260 Expressed in 227 organ(s), highest expression level in right testis
CleanExiHS_QSOX1
ExpressionAtlasiO00391 baseline and differential
GenevisibleiO00391 HS

Organism-specific databases

HPAiHPA042127
HPA056243

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

BioGridi111734, 21 interactors
IntActiO00391, 6 interactors
MINTiO00391
STRINGi9606.ENSP00000356574

Structurei

Secondary structure

1747
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO00391
SMRiO00391
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00391

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 156ThioredoxinPROSITE-ProRule annotationAdd BLAST121
Domaini396 – 503ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1731 Eukaryota
ENOG410XVJT LUCA
GeneTreeiENSGT00390000008045
HOGENOMiHOG000231631
HOVERGENiHBG080360
InParanoidiO00391
KOiK10758
OMAiKNFFGCT
OrthoDBiEOG091G038J
PhylomeDBiO00391
TreeFamiTF316749

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
IPR039798 Sulfhydryl_oxidase
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR22897 PTHR22897, 1 hit
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
SSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O00391-1) [UniParc]FASTAAdd to basket
Also known as: a, QSOX-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRRCNSGSGP PPSLLLLLLW LLAVPGANAA PRSALYSPSD PLTLLQADTV
60 70 80 90 100
RGAVLGSRSA WAVEFFASWC GHCIAFAPTW KALAEDVKAW RPALYLAALD
110 120 130 140 150
CAEETNSAVC RDFNIPGFPT VRFFKAFTKN GSGAVFPVAG ADVQTLRERL
160 170 180 190 200
IDALESHHDT WPPACPPLEP AKLEEIDGFF ARNNEEYLAL IFEKGGSYLG
210 220 230 240 250
REVALDLSQH KGVAVRRVLN TEANVVRKFG VTDFPSCYLL FRNGSVSRVP
260 270 280 290 300
VLMESRSFYT AYLQRLSGLT REAAQTTVAP TTANKIAPTV WKLADRSKIY
310 320 330 340 350
MADLESALHY ILRIEVGRFP VLEGQRLVAL KKFVAVLAKY FPGRPLVQNF
360 370 380 390 400
LHSVNEWLKR QKRNKIPYSF FKTALDDRKE GAVLAKKVNW IGCQGSEPHF
410 420 430 440 450
RGFPCSLWVL FHFLTVQAAR QNVDHSQEAA KAKEVLPAIR GYVHYFFGCR
460 470 480 490 500
DCASHFEQMA AASMHRVGSP NAAVLWLWSS HNRVNARLAG APSEDPQFPK
510 520 530 540 550
VQWPPRELCS ACHNERLDVP VWDVEATLNF LKAHFSPSNI ILDFPAAGSA
560 570 580 590 600
ARRDVQNVAA APELAMGALE LESRNSTLDP GKPEMMKSPT NTTPHVPAEG
610 620 630 640 650
PEASRPPKLH PGLRAAPGQE PPEHMAELQR NEQEQPLGQW HLSKRDTGAA
660 670 680 690 700
LLAESRAEKN RLWGPLEVRR VGRSSKQLVD IPEGQLEARA GRGRGQWLQV
710 720 730 740
LGGGFSYLDI SLCVGLYSLS FMGLLAMYTY FQAKIRALKG HAGHPAA
Length:747
Mass (Da):82,578
Last modified:June 1, 2001 - v3
Checksum:i52639D09A50E00C5
GO
Isoform 2 (identifier: O00391-2) [UniParc]FASTAAdd to basket
Also known as: b, QSOX-S

The sequence of this isoform differs from the canonical sequence as follows:
     603-604: AS → LI
     605-747: Missing.

Show »
Length:604
Mass (Da):66,861
Checksum:iB557EE4D1525E7FB
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A8MXT8A8MXT8_HUMAN
Sulfhydryl oxidase 1
QSOX1
155Annotation score:
H0Y5Z8H0Y5Z8_HUMAN
Sulfhydryl oxidase 1
QSOX1
72Annotation score:

Sequence cautioni

The sequence BAD92517 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027429114N → S. Corresponds to variant dbSNP:rs3894211Ensembl.1
Natural variantiVAR_027430200G → A3 PublicationsCorresponds to variant dbSNP:rs17855475Ensembl.1
Natural variantiVAR_027431256R → M. Corresponds to variant dbSNP:rs4360492Ensembl.1
Natural variantiVAR_027432294A → S. Corresponds to variant dbSNP:rs2278943Ensembl.1
Natural variantiVAR_027433444H → R1 PublicationCorresponds to variant dbSNP:rs12371Ensembl.1
Natural variantiVAR_027434591N → H. Corresponds to variant dbSNP:rs3738115Ensembl.1
Natural variantiVAR_053652605R → P. Corresponds to variant dbSNP:rs16855466Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020489603 – 604AS → LI in isoform 2. 2 Publications2
Alternative sequenceiVSP_020490605 – 747Missing in isoform 2. 2 PublicationsAdd BLAST143

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97276 mRNA Translation: AAC09010.2
AF361868 mRNA Translation: AAM00263.1
AY358941 mRNA Translation: AAQ89300.1
AB209280 mRNA Translation: BAD92517.1 Different initiation.
AL390718 Genomic DNA No translation available.
BC017692 mRNA Translation: AAH17692.1
BC100023 mRNA Translation: AAI00024.1
CCDSiCCDS1337.1 [O00391-1]
CCDS30950.1 [O00391-2]
RefSeqiNP_001004128.1, NM_001004128.2 [O00391-2]
NP_002817.2, NM_002826.4 [O00391-1]
UniGeneiHs.744925

Genome annotation databases

EnsembliENST00000367600; ENSP00000356572; ENSG00000116260 [O00391-2]
ENST00000367602; ENSP00000356574; ENSG00000116260 [O00391-1]
GeneIDi5768
KEGGihsa:5768
UCSCiuc001gny.4 human [O00391-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97276 mRNA Translation: AAC09010.2
AF361868 mRNA Translation: AAM00263.1
AY358941 mRNA Translation: AAQ89300.1
AB209280 mRNA Translation: BAD92517.1 Different initiation.
AL390718 Genomic DNA No translation available.
BC017692 mRNA Translation: AAH17692.1
BC100023 mRNA Translation: AAI00024.1
CCDSiCCDS1337.1 [O00391-1]
CCDS30950.1 [O00391-2]
RefSeqiNP_001004128.1, NM_001004128.2 [O00391-2]
NP_002817.2, NM_002826.4 [O00391-1]
UniGeneiHs.744925

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LLIX-ray2.05A286-546[»]
3LLKX-ray2.00A/B/C286-546[»]
3Q6OX-ray2.05A33-272[»]
4IJ3X-ray2.70A33-272[»]
ProteinModelPortaliO00391
SMRiO00391
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111734, 21 interactors
IntActiO00391, 6 interactors
MINTiO00391
STRINGi9606.ENSP00000356574

PTM databases

GlyConnecti1774
iPTMnetiO00391
PhosphoSitePlusiO00391

Polymorphism and mutation databases

BioMutaiQSOX1

Proteomic databases

EPDiO00391
MaxQBiO00391
PaxDbiO00391
PeptideAtlasiO00391
PRIDEiO00391
ProteomicsDBi47862
47863 [O00391-2]

Protocols and materials databases

DNASUi5768
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367600; ENSP00000356572; ENSG00000116260 [O00391-2]
ENST00000367602; ENSP00000356574; ENSG00000116260 [O00391-1]
GeneIDi5768
KEGGihsa:5768
UCSCiuc001gny.4 human [O00391-1]

Organism-specific databases

CTDi5768
DisGeNETi5768
EuPathDBiHostDB:ENSG00000116260.16
GeneCardsiQSOX1
HGNCiHGNC:9756 QSOX1
HPAiHPA042127
HPA056243
MIMi603120 gene
neXtProtiNX_O00391
OpenTargetsiENSG00000116260
PharmGKBiPA162400559
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1731 Eukaryota
ENOG410XVJT LUCA
GeneTreeiENSGT00390000008045
HOGENOMiHOG000231631
HOVERGENiHBG080360
InParanoidiO00391
KOiK10758
OMAiKNFFGCT
OrthoDBiEOG091G038J
PhylomeDBiO00391
TreeFamiTF316749

Enzyme and pathway databases

BRENDAi1.8.3.2 2681
ReactomeiR-HSA-114608 Platelet degranulation
R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-6798695 Neutrophil degranulation
R-HSA-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSiQSOX1 human
EvolutionaryTraceiO00391
GeneWikiiQSOX1
GenomeRNAii5768
PROiPR:O00391
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116260 Expressed in 227 organ(s), highest expression level in right testis
CleanExiHS_QSOX1
ExpressionAtlasiO00391 baseline and differential
GenevisibleiO00391 HS

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
IPR039798 Sulfhydryl_oxidase
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR22897 PTHR22897, 1 hit
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
SSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit
PS51352 THIOREDOXIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQSOX1_HUMAN
AccessioniPrimary (citable) accession number: O00391
Secondary accession number(s): Q59G29
, Q5T2X0, Q8TDL6, Q8WVP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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