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Entry version 86 (08 May 2019)
Sequence version 1 (01 Jul 1997)
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Protein

LINE-1 retrotransposable element ORF2 protein

Gene
N/A
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Has a reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Has also an endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome.3 Publications

Miscellaneous

Long interspersed element-1/LINE-1/L1 retrotransposons are present in more than 500'000 full (6 kb) or truncated copies in the human genome. Most of them are inactive but one estimate is that 80 to 100 of those elements could be transcribed, translated and active in any individual. An active LINE-1 encodes for 2 proteins translated from a single RNA containing two non-overlapping ORFs, ORF1 and ORF2. ORF2p is described in this entry as a representative of all ORF2p potentially expressed by active elements. ORF1p is described in the related entry AC Q9UN81.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi600Magnesium; catalyticPROSITE-ProRule annotation1
Metal bindingi702Magnesium; catalyticPROSITE-ProRule annotation1
Metal bindingi703Magnesium; catalyticPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionEndonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase
Biological processDNA recombination
LigandMagnesium, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
LINE-1 retrotransposable element ORF2 protein
Short name:
ORF2p
Including the following 2 domains:
Reverse transcriptase (EC:2.7.7.49)
Endonuclease (EC:3.1.21.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_O00370

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi14N → A: Loss of endonuclease activity and reduced transposition efficiency. 1 Publication1
Mutagenesisi43E → A: Loss of endonuclease activity. 1 Publication1
Mutagenesisi145D → A: Loss of endonuclease activity and reduced transposition efficiency. 1 Publication1
Mutagenesisi205D → G: Loss of endonuclease activity and reduced transposition efficiency. 1 Publication1
Mutagenesisi230H → A: Loss of endonuclease activity and reduced transposition efficiency. 1 Publication1
Mutagenesisi703D → Y: Reduced transposition efficiency. 1 Publication1

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
-

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004250821 – 1275LINE-1 retrotransposable element ORF2 proteinAdd BLAST1275

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O00370

PeptideAtlas

More...
PeptideAtlasi
O00370

PRoteomics IDEntifications database

More...
PRIDEi
O00370

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O00370

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
O00370

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with MOV10.

By similarity

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
O00370, 3 interactors

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O00370

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini498 – 773Reverse transcriptasePROSITE-ProRule annotationAdd BLAST276
Domaini1242 – 1260DUF1725Add BLAST19

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 239Endonuclease activityAdd BLAST239

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG410IG0K Eukaryota
ENOG410Y9TZ LUCA

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.60.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013544 DUF1725
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR000477 RT_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08333 DUF1725, 1 hit
PF03372 Exo_endo_phos, 1 hit
PF00078 RVT_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56219 SSF56219, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50878 RT_POL, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O00370-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTGSNSHITI LTLNVNGLNS PIKRHRLASW IKSQDPSVCC IQETHLTCRD
60 70 80 90 100
THRLKIKGWR KIYQANGKQK KAGVAILVSD KTDFKPTKIK RDKEGHYIMV
110 120 130 140 150
KGSIQQEELT ILNIYAPNTG APRFIKQVLS DLQRDLDSHT LIMGDFNTPL
160 170 180 190 200
SILDRSTRQK VNKDTQELNS ALHQTDLIDI YRTLHPKSTE YTFFSAPHHT
210 220 230 240 250
YSKIDHIVGS KALLSKCKRT EIITNYLSDH SAIKLELRIK NLTQSRSTTW
260 270 280 290 300
KLNNLLLNDY WVHNEMKAEI KMFFETNENK DTTYQNLWDA FKAVCRGKFI
310 320 330 340 350
ALNAYKRKQE RSKIDTLTSQ LKELEKQEQT HSKASRRQEI TKIRAELKEI
360 370 380 390 400
ETQKTLQKIN ESRSWFFERI NKIDRPLARL IKKKREKNQI DTIKNDKGDI
410 420 430 440 450
TTDPTEIQTT IREYYKHLYA NKLENLEEMD TFLDTYTLPR LNQEEVESLN
460 470 480 490 500
RPITGSEIVA IINSLPTKKS PGPDGFTAEF YQRYKEELVP FLLKLFQSIE
510 520 530 540 550
KEGILPNSFY EASIILIPKP GRDTTKKENF RPISLMNIDA KILNKILANR
560 570 580 590 600
IQQHIKKLIH HDQVGFIPGM QGWFNIRKSI NVIQHINRAK DKNHVIISID
610 620 630 640 650
AEKAFDKIQQ PFMLKTLNKL GIDGMYLKII RAIYDKPTAN IILNGQKLEA
660 670 680 690 700
FPLKTGTRQG CPLSPLLFNI VLEVLARAIR QEKEIKGIQL GKEEVKLSLF
710 720 730 740 750
ADDMIVYLEN PIVSAQNLLK LISNFSKVSG YKINVQKSQA FLYNNNRQTE
760 770 780 790 800
SQIMGELPFT IASKRIKYLG IQLTRDVKDL FKENYKPLLK EIKEDTNKWK
810 820 830 840 850
NIPCSWVGRI NIVKMAILPK VIYRFNAIPI KLPMTFFTEL EKTTLKFIWN
860 870 880 890 900
QKRARIAKSI LSQKNKAGGI TLPDFKLYYK ATVTKTAWYW YQNRDIDQWN
910 920 930 940 950
RTEPSEIMPH IYNYLIFDKP EKNKQWGKDS LLNKWCWENW LAICRKLKLD
960 970 980 990 1000
PFLTPYTKIN SRWIKDLNVK PKTIKTLEEN LGITIQDIGV GKDFMSKTPK
1010 1020 1030 1040 1050
AMATKDKIDK WDLIKLKSFC TAKETTIRVN RQPTTWEKIF ATYSSDKGLI
1060 1070 1080 1090 1100
SRIYNELKQI YKKKTNNPIK KWAKDMNRHF SKEDIYAAKK HMKKCSSSLA
1110 1120 1130 1140 1150
IREMQIKTTM RYHLTPVRMA IIKKSGNNRC WRGCGEIGTL VHCWWDCKLV
1160 1170 1180 1190 1200
QPLWKSVWRF LRDLELEIPF DPAIPLLGIY PKDYKSCCYK DTCTRMFIAA
1210 1220 1230 1240 1250
LFTIAKTWNQ PNCPTMIDWI KKMWHIYTME YYAAIKNDEF ISFVGTWMKL
1260 1270
ETIILSKLSQ EQKTKHRIFS LIGGN
Length:1,275
Mass (Da):149,012
Last modified:July 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i588703688E7129FF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U93569 Genomic DNA Translation: AAC51271.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B28096
S23650

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93569 Genomic DNA Translation: AAC51271.1
PIRiB28096
S23650

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VYBX-ray1.80A/B1-238[»]
2V0RX-ray2.30A/B1-238[»]
2V0SX-ray1.80A1-238[»]
SMRiO00370
ModBaseiSearch...

Protein-protein interaction databases

IntActiO00370, 3 interactors

PTM databases

iPTMnetiO00370
PhosphoSitePlusiO00370

Polymorphism and mutation databases

BioMutai-

Proteomic databases

PaxDbiO00370
PeptideAtlasiO00370
PRIDEiO00370

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

neXtProtiNX_O00370

Phylogenomic databases

eggNOGiENOG410IG0K Eukaryota
ENOG410Y9TZ LUCA

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR013544 DUF1725
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR000477 RT_dom
PfamiView protein in Pfam
PF08333 DUF1725, 1 hit
PF03372 Exo_endo_phos, 1 hit
PF00078 RVT_1, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS50878 RT_POL, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiLORF2_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O00370
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: July 1, 1997
Last modified: May 8, 2019
This is version 86 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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