UniProtKB - O00341 (EAA5_HUMAN)
Protein
Excitatory amino acid transporter 5
Gene
SLC1A7
Organism
Homo sapiens (Human)
Status
Functioni
Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.
GO - Molecular functioni
- L-glutamate transmembrane transporter activity Source: UniProtKB
- symporter activity Source: UniProtKB-KW
GO - Biological processi
- dicarboxylic acid transport Source: UniProtKB
- glutamate secretion Source: Reactome
- ion transport Source: Reactome
- neurotransmitter reuptake Source: SynGO
- neurotransmitter uptake Source: SynGO
Keywordsi
Biological process | Symport, Transport |
Enzyme and pathway databases
PathwayCommonsi | O00341 |
Reactomei | R-HSA-210500, Glutamate Neurotransmitter Release Cycle R-HSA-425393, Transport of inorganic cations/anions and amino acids/oligopeptides |
Protein family/group databases
TCDBi | 2.A.23.2.5, the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family |
Names & Taxonomyi
Protein namesi | Recommended name: Excitatory amino acid transporter 5Alternative name(s): Retinal glutamate transporter Solute carrier family 1 member 7 |
Gene namesi | Name:SLC1A7 Synonyms:EAAT5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10945, SLC1A7 |
MIMi | 604471, gene |
neXtProti | NX_O00341 |
VEuPathDBi | HostDB:ENSG00000162383.11 |
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- presynapse Source: GOC
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 60 – 80 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 94 – 114 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 115 – 216 | ExtracellularSequence analysisAdd BLAST | 102 | |
Transmembranei | 217 – 237 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 260 – 280 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 300 – 320 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 330 – 350 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 372 – 392 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 414 – 434 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 457 – 477 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 6512 |
OpenTargetsi | ENSG00000162383 |
PharmGKBi | PA35832 |
Miscellaneous databases
Pharosi | O00341, Tchem |
Chemistry databases
DrugBanki | DB00142, Glutamic acid |
GuidetoPHARMACOLOGYi | 872 |
Genetic variation databases
BioMutai | SLC1A7 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202073 | 1 – 560 | Excitatory amino acid transporter 5Add BLAST | 560 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 191 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | O00341 |
jPOSTi | O00341 |
MassIVEi | O00341 |
PaxDbi | O00341 |
PeptideAtlasi | O00341 |
PRIDEi | O00341 |
ProteomicsDBi | 47860 [O00341-1] 79253 |
PTM databases
GlyGeni | O00341, 1 site |
iPTMneti | O00341 |
PhosphoSitePlusi | O00341 |
Expressioni
Tissue specificityi
Expressed primarily in retina. Detectable in liver, heart, muscle and brain.
Gene expression databases
Bgeei | ENSG00000162383, Expressed in pigmented layer of retina and 125 other tissues |
ExpressionAtlasi | O00341, baseline and differential |
Genevisiblei | O00341, HS |
Organism-specific databases
HPAi | ENSG00000162383, Tissue enriched (retina) |
Interactioni
Subunit structurei
Interacts with the PDZ domains of DLG4.
Protein-protein interaction databases
BioGRIDi | 112403, 2 interactors |
IntActi | O00341, 2 interactors |
Miscellaneous databases
RNActi | O00341, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A7 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00940000156073 |
InParanoidi | O00341 |
PhylomeDBi | O00341 |
TreeFami | TF315206 |
Family and domain databases
Gene3Di | 1.10.3860.10, 2 hits |
InterProi | View protein in InterPro IPR001991, Na-dicarboxylate_symporter IPR018107, Na-dicarboxylate_symporter_CS IPR036458, Na:dicarbo_symporter_sf |
Pfami | View protein in Pfam PF00375, SDF, 1 hit |
SUPFAMi | SSF118215, SSF118215, 1 hit |
PROSITEi | View protein in PROSITE PS00713, NA_DICARBOXYL_SYMP_1, 1 hit PS00714, NA_DICARBOXYL_SYMP_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O00341-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF
60 70 80 90 100
QFPGELLMRM LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT
110 120 130 140 150
FMAVIVGIFM VSIIHPGSAA QKETTEQSGK PIMSSADALL DLIRNMFPAN
160 170 180 190 200
LVEATFKQYR TKTTPVVKSP KVAPEEAPPR RILIYGVQEE NGSHVQNFAL
210 220 230 240 250
DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG DSGAPLVSFC
260 270 280 290 300
QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV
310 320 330 340 350
VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI
360 370 380 390 400
TFKCLLENNH IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL
410 420 430 440 450
DFGQIITISI TATAASIGAA GIPQAGLVTM VIVLTSVGLP TDDITLIIAV
460 470 480 490 500
DWALDRFRTM INVLGDALAA GIMAHICRKD FARDTGTEKL LPCETKPVSL
510 520 530 540 550
QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE LPAASLNHCT
560
IQISELETNV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1T0D3 | F1T0D3_HUMAN | Amino acid transporter | SLC1A7 | 450 | Annotation score: | ||
F1T0D2 | F1T0D2_HUMAN | Amino acid transporter | SLC1A7 | 158 | Annotation score: | ||
F1T0D4 | F1T0D4_HUMAN | Amino acid transporter | SLC1A7 | 472 | Annotation score: | ||
A0A087WUF9 | A0A087WUF9_HUMAN | Amino acid transporter | SLC1A7 | 619 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | A → T in AAB53971 (PubMed:9108121).Curated | 1 | |
Sequence conflicti | 70 | V → F in AAB53971 (PubMed:9108121).Curated | 1 | |
Sequence conflicti | 244 | A → G in AAB53971 (PubMed:9108121).Curated | 1 | |
Sequence conflicti | 412 | A → G in AAB53971 (PubMed:9108121).Curated | 1 | |
Sequence conflicti | 449 | A → G in AAB53971 (PubMed:9108121).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035707 | 41 | R → C in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs375136400Ensembl. | 1 | |
Natural variantiVAR_052488 | 537 | Q → R3 PublicationsCorresponds to variant dbSNP:rs1288401Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056560 | 145 – 158 | NMFPA…ATFKQ → QKEESWRNGPKGPG in isoform 2. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_056561 | 159 – 560 | Missing in isoform 2. 1 PublicationAdd BLAST | 402 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U76362 mRNA Translation: AAB53971.1 AL445183 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06754.1 CH471059 Genomic DNA Translation: EAX06755.1 BC000651 mRNA Translation: AAH00651.1 BC012119 mRNA Translation: AAH12119.1 BC017242 mRNA Translation: AAH17242.1 |
CCDSi | CCDS574.1 [O00341-1] CCDS72798.1 [O00341-2] |
RefSeqi | NP_001274525.1, NM_001287596.1 [O00341-2] NP_001274526.1, NM_001287597.1 NP_006662.3, NM_006671.5 [O00341-1] |
Genome annotation databases
Ensembli | ENST00000371491; ENSP00000360546; ENSG00000162383 [O00341-2] ENST00000371494; ENSP00000360549; ENSG00000162383 [O00341-1] |
GeneIDi | 6512 |
KEGGi | hsa:6512 |
UCSCi | uc001cuy.5, human [O00341-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U76362 mRNA Translation: AAB53971.1 AL445183 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06754.1 CH471059 Genomic DNA Translation: EAX06755.1 BC000651 mRNA Translation: AAH00651.1 BC012119 mRNA Translation: AAH12119.1 BC017242 mRNA Translation: AAH17242.1 |
CCDSi | CCDS574.1 [O00341-1] CCDS72798.1 [O00341-2] |
RefSeqi | NP_001274525.1, NM_001287596.1 [O00341-2] NP_001274526.1, NM_001287597.1 NP_006662.3, NM_006671.5 [O00341-1] |
3D structure databases
SMRi | O00341 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112403, 2 interactors |
IntActi | O00341, 2 interactors |
Chemistry databases
DrugBanki | DB00142, Glutamic acid |
GuidetoPHARMACOLOGYi | 872 |
Protein family/group databases
TCDBi | 2.A.23.2.5, the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family |
PTM databases
GlyGeni | O00341, 1 site |
iPTMneti | O00341 |
PhosphoSitePlusi | O00341 |
Genetic variation databases
BioMutai | SLC1A7 |
Proteomic databases
EPDi | O00341 |
jPOSTi | O00341 |
MassIVEi | O00341 |
PaxDbi | O00341 |
PeptideAtlasi | O00341 |
PRIDEi | O00341 |
ProteomicsDBi | 47860 [O00341-1] 79253 |
Protocols and materials databases
Antibodypediai | 46889, 122 antibodies |
DNASUi | 6512 |
Genome annotation databases
Ensembli | ENST00000371491; ENSP00000360546; ENSG00000162383 [O00341-2] ENST00000371494; ENSP00000360549; ENSG00000162383 [O00341-1] |
GeneIDi | 6512 |
KEGGi | hsa:6512 |
UCSCi | uc001cuy.5, human [O00341-1] |
Organism-specific databases
CTDi | 6512 |
DisGeNETi | 6512 |
GeneCardsi | SLC1A7 |
HGNCi | HGNC:10945, SLC1A7 |
HPAi | ENSG00000162383, Tissue enriched (retina) |
MIMi | 604471, gene |
neXtProti | NX_O00341 |
OpenTargetsi | ENSG00000162383 |
PharmGKBi | PA35832 |
VEuPathDBi | HostDB:ENSG00000162383.11 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00940000156073 |
InParanoidi | O00341 |
PhylomeDBi | O00341 |
TreeFami | TF315206 |
Enzyme and pathway databases
PathwayCommonsi | O00341 |
Reactomei | R-HSA-210500, Glutamate Neurotransmitter Release Cycle R-HSA-425393, Transport of inorganic cations/anions and amino acids/oligopeptides |
Miscellaneous databases
BioGRID-ORCSi | 6512, 5 hits in 877 CRISPR screens |
GeneWikii | Excitatory_amino-acid_transporter_5 |
GenomeRNAii | 6512 |
Pharosi | O00341, Tchem |
PROi | PR:O00341 |
RNActi | O00341, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000162383, Expressed in pigmented layer of retina and 125 other tissues |
ExpressionAtlasi | O00341, baseline and differential |
Genevisiblei | O00341, HS |
Family and domain databases
Gene3Di | 1.10.3860.10, 2 hits |
InterProi | View protein in InterPro IPR001991, Na-dicarboxylate_symporter IPR018107, Na-dicarboxylate_symporter_CS IPR036458, Na:dicarbo_symporter_sf |
Pfami | View protein in Pfam PF00375, SDF, 1 hit |
SUPFAMi | SSF118215, SSF118215, 1 hit |
PROSITEi | View protein in PROSITE PS00713, NA_DICARBOXYL_SYMP_1, 1 hit PS00714, NA_DICARBOXYL_SYMP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EAA5_HUMAN | |
Accessioni | O00341Primary (citable) accession number: O00341 Secondary accession number(s): Q5VVZ0, Q969Z8, Q9BW45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | September 23, 2008 | |
Last modified: | February 10, 2021 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants