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Entry version 184 (08 May 2019)
Sequence version 1 (01 Jul 1997)
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Protein

Transcription elongation factor SPT5

Gene

SUPT5H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.19 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

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BioCyci
MetaCyc:G66-31601-MONOMER

Reactome - a knowledgebase of biological pathways and processes

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Reactomei
R-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE

SIGNOR Signaling Network Open Resource

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SIGNORi
O00267

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription elongation factor SPT5
Short name:
hSPT5
Alternative name(s):
DRB sensitivity-inducing factor 160 kDa subunit
Short name:
DSIF p160
DRB sensitivity-inducing factor large subunit
Short name:
DSIF large subunit
Tat-cotransactivator 1 protein
Short name:
Tat-CT1 protein
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SUPT5H
Synonyms:SPT5, SPT5H
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:11469 SUPT5H

Online Mendelian Inheritance in Man (OMIM)

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MIMi
602102 gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_O00267

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi681R → A: Enhances interactions with CDK9 and RNA polymerase II and enhances transcriptional elongation; when associated with A-696 and A-698. 1 Publication1
Mutagenesisi681R → K: Increases promoter association and enhances transcriptional elongation; when associated with K-696 and K-698. 1 Publication1
Mutagenesisi696R → A: Enhances interactions with CDK9 and RNA polymerase II and enhances transcriptional elongation; when associated with A-681 and A-698. 1 Publication1
Mutagenesisi696R → K: Increases promoter association and enhances transcriptional elongation; when associated with K-681 and K-698. 1 Publication1
Mutagenesisi698R → A: Enhances transcriptional elongation. Enhances interactions with CDK9 and RNA polymerase II and enhances transcriptional elongation; when associated with A-681 and A-696. 1 Publication1
Mutagenesisi698R → K: Increases promoter association and enhances transcriptional elongation; when associated with K-681 and K-696. 1 Publication1
Mutagenesisi1002G → D: Defective in regulation of transcriptional elongation. 1 Publication1

Organism-specific databases

DisGeNET

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DisGeNETi
6829

Open Targets

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OpenTargetsi
ENSG00000196235

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA36255

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
SUPT5H

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002084681 – 1087Transcription elongation factor SPT5Add BLAST1087

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei32PhosphoserineBy similarity1
Modified residuei36PhosphoserineBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki143Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei666PhosphoserineCombined sources1
Modified residuei681Asymmetric dimethylarginine; by PRMT1; alternate1 Publication1
Modified residuei681Omega-N-methylarginine; by PRMT1; alternate1 Publication1
Modified residuei686PhosphoserineCombined sources1
Modified residuei696Asymmetric dimethylarginine; by PRMT1; alternate1 Publication1
Modified residuei696Omega-N-methylarginine; by PRMT1; alternate1 Publication1
Modified residuei698Asymmetric dimethylarginine; by PRMT1; alternate1 Publication1
Modified residuei698Omega-N-methylarginine; by PRMT1 and PRMT5; alternate1 Publication1
Modified residuei698Symmetric dimethylarginine; by PRMT5; alternate1 Publication1
Modified residuei718N6-acetyllysineBy similarity1
Modified residuei775Phosphothreonine; by CDK91 Publication1
Modified residuei784Phosphothreonine; by CDK91 Publication1
Modified residuei789PhosphoserineCombined sources1
Modified residuei791PhosphothreonineCombined sources1
Modified residuei804PhosphoserineCombined sources1
Modified residuei806PhosphothreonineCombined sources1
Modified residuei1034PhosphothreonineCombined sources1
Cross-linki1037Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.1 Publication
Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.8 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
O00267

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
O00267

MaxQB - The MaxQuant DataBase

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MaxQBi
O00267

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
O00267

PeptideAtlas

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PeptideAtlasi
O00267

PRoteomics IDEntifications database

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PRIDEi
O00267

ProteomicsDB human proteome resource

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ProteomicsDBi
47816
47817 [O00267-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
O00267

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
O00267

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000196235 Expressed in 229 organ(s), highest expression level in testis

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
O00267 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
O00267 HS

Organism-specific databases

Human Protein Atlas

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HPAi
CAB034370
HPA029273

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with SUPT4H1 to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of NELFA, NELFB, NELFD and NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with PRMT1/HRMT1L2, HTATSF1/TATSF1, RNGTT/CAP1A, PRMT5/SKB1, SUPT6H, and can interact with PIN1. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with MCM3AP isoform GANP (PubMed:23652018).16 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
112697, 164 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
O00267

Database of interacting proteins

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DIPi
DIP-29014N

Protein interaction database and analysis system

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IntActi
O00267, 82 interactors

Molecular INTeraction database

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MINTi
O00267

STRING: functional protein association networks

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STRINGi
9606.ENSP00000470252

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11087
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DO3NMR-A462-523[»]
2E6ZNMR-A420-471[»]
2E70NMR-A694-757[»]
3H7HX-ray1.55B176-273[»]
4L1UX-ray2.42G/H/I/J778-790[»]
5OHOX-ray1.60A/B536-646[»]
5OHQX-ray1.10A979-1087[»]
5OIKelectron microscopy3.70Z1-1087[»]
5U98X-ray2.00C/F980-988[»]
6EQYNMR-A522-647[»]
6ER0NMR-A961-1087[»]
6GMHelectron microscopy3.10Z1-1087[»]
6GMLelectron microscopy3.20Z1-1087[»]

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O00267

Database of comparative protein structure models

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ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
O00267

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini273 – 306KOW 1Add BLAST34
Domaini420 – 451KOW 2Add BLAST32
Domaini472 – 503KOW 3Add BLAST32
Domaini594 – 627KOW 4Add BLAST34
Domaini704 – 737KOW 5Add BLAST34
<p>This subsection of the ‘Family and Domains’ section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati754 – 759CTR1-1; approximate6
Repeati760 – 765CTR1-26
Repeati766 – 771CTR1-36
Repeati772 – 778CTR1-47
Repeati781 – 787CTR1-57
Repeati788 – 794CTR1-67
Repeati796 – 802CTR1-77
Repeati803 – 809CTR1-87
Repeati811 – 817CTR1-97
Repeati844 – 851CTR2-18
Repeati854 – 862CTR2-2; approximate9
Repeati863 – 869CTR2-3; approximate7
Repeati881 – 885CTR2-4; half-length5
Repeati896 – 902CTR2-5; approximate7
Repeati904 – 911CTR2-68
Repeati916 – 921CTR2-7; approximate6
Repeati924 – 930CTR2-87
Repeati932 – 939CTR2-98
Repeati943 – 950CTR2-108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni176 – 270Interaction with SUPT4H1Add BLAST95
Regioni313 – 420Interaction with RNA polymerase IIAdd BLAST108
Regioni754 – 8179 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1Add BLAST64
Regioni844 – 95010 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2Add BLAST107

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi11 – 106Glu-richAdd BLAST96
Compositional biasi844 – 968Pro-richAdd BLAST125

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the SPT5 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1999 Eukaryota
COG0250 LUCA
COG5164 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00440000037640

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000038564

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
O00267

KEGG Orthology (KO)

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KOi
K15172

Identification of Orthologs from Complete Genome Data

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OMAi
RGKYQGD

Database of Orthologous Groups

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OrthoDBi
828863at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
O00267

TreeFam database of animal gene trees

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TreeFami
TF105730

Family and domain databases

Conserved Domains Database

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CDDi
cd06081 KOW_Spt5_1, 1 hit
cd06082 KOW_Spt5_2, 1 hit
cd06083 KOW_Spt5_3, 1 hit
cd06084 KOW_Spt5_4, 1 hit
cd06085 KOW_Spt5_5, 1 hit
cd06086 KOW_Spt5_6, 1 hit
cd09888 NGN_Euk, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
2.30.30.30, 3 hits
3.30.70.940, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR005824 KOW
IPR041973 KOW_Spt5_1
IPR041975 KOW_Spt5_2
IPR041976 KOW_Spt5_3
IPR041977 KOW_Spt5_4
IPR041978 KOW_Spt5_5
IPR041980 KOW_Spt5_6
IPR005100 NGN-domain
IPR006645 NGN_dom
IPR036735 NGN_dom_sf
IPR039385 NGN_Euk
IPR014722 Rib_L2_dom2
IPR039659 SPT5
IPR024945 Spt5_C_dom
IPR022581 Spt5_N
IPR017071 TF_Spt5_eukaryote
IPR008991 Translation_prot_SH3-like_sf

The PANTHER Classification System

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PANTHERi
PTHR11125 PTHR11125, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF00467 KOW, 1 hit
PF03439 Spt5-NGN, 1 hit
PF11942 Spt5_N, 1 hit

PIRSF; a whole-protein classification database

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PIRSFi
PIRSF036945 Spt5, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM01104 CTD, 1 hit
SM00739 KOW, 6 hits
SM00738 NGN, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF50104 SSF50104, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O00267-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE
60 70 80 90 100
EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDED QWEDGAEDIL
110 120 130 140 150
EKEEIEASNI DNVVLDEDRS GARRLQNLWR DQREEELGEY YMKKYAKSSV
160 170 180 190 200
GETVYGGSDE LSDDITQQQL LPGVKDPNLW TVKCKIGEER ATAISLMRKF
210 220 230 240 250
IAYQFTDTPL QIKSVVAPEH VKGYIYVEAY KQTHVKQAIE GVGNLRLGYW
260 270 280 290 300
NQQMVPIKEM TDVLKVVKEV ANLKPKSWVR LKRGIYKDDI AQVDYVEPSQ
310 320 330 340 350
NTISLKMIPR IDYDRIKARM SLKDWFAKRK KFKRPPQRLF DAEKIRSLGG
360 370 380 390 400
DVASDGDFLI FEGNRYSRKG FLFKSFAMSA VITEGVKPTL SELEKFEDQP
410 420 430 440 450
EGIDLEVVTE STGKEREHNF QPGDNVEVCE GELINLQGKI LSVDGNKITI
460 470 480 490 500
MPKHEDLKDM LEFPAQELRK YFKMGDHVKV IAGRFEGDTG LIVRVEENFV
510 520 530 540 550
ILFSDLTMHE LKVLPRDLQL CSETASGVDV GGQHEWGELV QLDPQTVGVI
560 570 580 590 600
VRLERETFQV LNMYGKVVTV RHQAVTRKKD NRFAVALDSE QNNIHVKDIV
610 620 630 640 650
KVIDGPHSGR EGEIRHLFRS FAFLHCKKLV ENGGMFVCKT RHLVLAGGSK
660 670 680 690 700
PRDVTNFTVG GFAPMSPRIS SPMHPSAGGQ RGGFGSPGGG SGGMSRGRGR
710 720 730 740 750
RDNELIGQTV RISQGPYKGY IGVVKDATES TARVELHSTC QTISVDRQRL
760 770 780 790 800
TTVGSRRPGG MTSTYGRTPM YGSQTPMYGS GSRTPMYGSQ TPLQDGSRTP
810 820 830 840 850
HYGSQTPLHD GSRTPAQSGA WDPNNPNTPS RAEEEYEYAF DDEPTPSPQA
860 870 880 890 900
YGGTPNPQTP GYPDPSSPQV NPQYNPQTPG TPAMYNTDQF SPYAAPSPQG
910 920 930 940 950
SYQPSPSPQS YHQVAPSPAG YQNTHSPASY HPTPSPMAYQ ASPSPSPVGY
960 970 980 990 1000
SPMTPGAPSP GGYNPHTPGS GIEQNSSDWV TTDIQVKVRD TYLDTQVVGQ
1010 1020 1030 1040 1050
TGVIRSVTGG MCSVYLKDSE KVVSISSEHL EPITPTKNNK VKVILGEDRE
1060 1070 1080
ATGVLLSIDG EDGIVRMDLD EQLKILNLRF LGKLLEA
Length:1,087
Mass (Da):121,000
Last modified:July 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iEC3F402A670A5B7D
GO
Isoform 2 (identifier: O00267-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-106: Missing.

Show »
Length:1,083
Mass (Da):120,499
Checksum:i1B62A9E8011EB918
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QYL9M0QYL9_HUMAN
Transcription elongation factor SPT...
SUPT5H
164Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R2M5M0R2M5_HUMAN
Transcription elongation factor SPT...
SUPT5H
102Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R105M0R105_HUMAN
Transcription elongation factor SPT...
SUPT5H
54Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAD92494 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti181T → I in AAC51102 (PubMed:8975720).Curated1
Sequence conflicti483G → A in AAC51102 (PubMed:8975720).Curated1
Sequence conflicti820A → G in AAC51102 (PubMed:8975720).Curated1
Sequence conflicti846P → R in BAA24075 (PubMed:9450929).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_016282103 – 106Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U56402 mRNA Translation: AAC51102.1
Y12790 mRNA Translation: CAA73326.1
AB000516 mRNA Translation: BAA24075.1
AF040253 mRNA Translation: AAD02179.1
AB209257 mRNA Translation: BAD92494.1 Different initiation.
BC024203 mRNA Translation: AAH24203.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS12536.1 [O00267-1]
CCDS46072.1 [O00267-2]

NCBI Reference Sequences

More...
RefSeqi
NP_001104490.1, NM_001111020.2 [O00267-1]
NP_001124296.1, NM_001130824.1 [O00267-1]
NP_001124297.1, NM_001130825.1 [O00267-2]
NP_001306919.1, NM_001319990.1 [O00267-1]
NP_001306920.1, NM_001319991.1 [O00267-2]
NP_003160.2, NM_003169.3 [O00267-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000359191; ENSP00000352117; ENSG00000196235 [O00267-2]
ENST00000402194; ENSP00000384505; ENSG00000196235 [O00267-2]
ENST00000432763; ENSP00000404029; ENSG00000196235 [O00267-1]
ENST00000598725; ENSP00000469090; ENSG00000196235 [O00267-1]
ENST00000599117; ENSP00000470252; ENSG00000196235 [O00267-1]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
6829

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:6829

UCSC genome browser

More...
UCSCi
uc002olo.5 human [O00267-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56402 mRNA Translation: AAC51102.1
Y12790 mRNA Translation: CAA73326.1
AB000516 mRNA Translation: BAA24075.1
AF040253 mRNA Translation: AAD02179.1
AB209257 mRNA Translation: BAD92494.1 Different initiation.
BC024203 mRNA Translation: AAH24203.1
CCDSiCCDS12536.1 [O00267-1]
CCDS46072.1 [O00267-2]
RefSeqiNP_001104490.1, NM_001111020.2 [O00267-1]
NP_001124296.1, NM_001130824.1 [O00267-1]
NP_001124297.1, NM_001130825.1 [O00267-2]
NP_001306919.1, NM_001319990.1 [O00267-1]
NP_001306920.1, NM_001319991.1 [O00267-2]
NP_003160.2, NM_003169.3 [O00267-1]

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DO3NMR-A462-523[»]
2E6ZNMR-A420-471[»]
2E70NMR-A694-757[»]
3H7HX-ray1.55B176-273[»]
4L1UX-ray2.42G/H/I/J778-790[»]
5OHOX-ray1.60A/B536-646[»]
5OHQX-ray1.10A979-1087[»]
5OIKelectron microscopy3.70Z1-1087[»]
5U98X-ray2.00C/F980-988[»]
6EQYNMR-A522-647[»]
6ER0NMR-A961-1087[»]
6GMHelectron microscopy3.10Z1-1087[»]
6GMLelectron microscopy3.20Z1-1087[»]
SMRiO00267
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112697, 164 interactors
CORUMiO00267
DIPiDIP-29014N
IntActiO00267, 82 interactors
MINTiO00267
STRINGi9606.ENSP00000470252

PTM databases

iPTMnetiO00267
PhosphoSitePlusiO00267

Polymorphism and mutation databases

BioMutaiSUPT5H

Proteomic databases

EPDiO00267
jPOSTiO00267
MaxQBiO00267
PaxDbiO00267
PeptideAtlasiO00267
PRIDEiO00267
ProteomicsDBi47816
47817 [O00267-2]

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
6829
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359191; ENSP00000352117; ENSG00000196235 [O00267-2]
ENST00000402194; ENSP00000384505; ENSG00000196235 [O00267-2]
ENST00000432763; ENSP00000404029; ENSG00000196235 [O00267-1]
ENST00000598725; ENSP00000469090; ENSG00000196235 [O00267-1]
ENST00000599117; ENSP00000470252; ENSG00000196235 [O00267-1]
GeneIDi6829
KEGGihsa:6829
UCSCiuc002olo.5 human [O00267-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
6829
DisGeNETi6829

GeneCards: human genes, protein and diseases

More...
GeneCardsi
SUPT5H
HGNCiHGNC:11469 SUPT5H
HPAiCAB034370
HPA029273
MIMi602102 gene
neXtProtiNX_O00267
OpenTargetsiENSG00000196235
PharmGKBiPA36255

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1999 Eukaryota
COG0250 LUCA
COG5164 LUCA
GeneTreeiENSGT00440000037640
HOGENOMiHOG000038564
InParanoidiO00267
KOiK15172
OMAiRGKYQGD
OrthoDBi828863at2759
PhylomeDBiO00267
TreeFamiTF105730

Enzyme and pathway databases

BioCyciMetaCyc:G66-31601-MONOMER
ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
SIGNORiO00267

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
SUPT5H human
EvolutionaryTraceiO00267

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
SUPT5H

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
6829

Protein Ontology

More...
PROi
PR:O00267

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000196235 Expressed in 229 organ(s), highest expression level in testis
ExpressionAtlasiO00267 baseline and differential
GenevisibleiO00267 HS

Family and domain databases

CDDicd06081 KOW_Spt5_1, 1 hit
cd06082 KOW_Spt5_2, 1 hit
cd06083 KOW_Spt5_3, 1 hit
cd06084 KOW_Spt5_4, 1 hit
cd06085 KOW_Spt5_5, 1 hit
cd06086 KOW_Spt5_6, 1 hit
cd09888 NGN_Euk, 1 hit
Gene3Di2.30.30.30, 3 hits
3.30.70.940, 1 hit
InterProiView protein in InterPro
IPR005824 KOW
IPR041973 KOW_Spt5_1
IPR041975 KOW_Spt5_2
IPR041976 KOW_Spt5_3
IPR041977 KOW_Spt5_4
IPR041978 KOW_Spt5_5
IPR041980 KOW_Spt5_6
IPR005100 NGN-domain
IPR006645 NGN_dom
IPR036735 NGN_dom_sf
IPR039385 NGN_Euk
IPR014722 Rib_L2_dom2
IPR039659 SPT5
IPR024945 Spt5_C_dom
IPR022581 Spt5_N
IPR017071 TF_Spt5_eukaryote
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR11125 PTHR11125, 1 hit
PfamiView protein in Pfam
PF00467 KOW, 1 hit
PF03439 Spt5-NGN, 1 hit
PF11942 Spt5_N, 1 hit
PIRSFiPIRSF036945 Spt5, 1 hit
SMARTiView protein in SMART
SM01104 CTD, 1 hit
SM00739 KOW, 6 hits
SM00738 NGN, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPT5H_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O00267
Secondary accession number(s): O43279, Q59G52, Q99639
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 1, 1997
Last modified: May 8, 2019
This is version 184 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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