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Protein

Proteinase-activated receptor 3

Gene

F2RL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.1 Publication

GO - Molecular functioni

  • G-protein coupled receptor activity Source: GO_Central
  • phosphatidylinositol phospholipase C activity Source: ProtInc
  • thrombin-activated receptor activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBlood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiR-HSA-375276 Peptide ligand-binding receptors
R-HSA-416476 G alpha (q) signalling events
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
SIGNORiO00254

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 3
Short name:
PAR-3
Alternative name(s):
Coagulation factor II receptor-like 2
Thrombin receptor-like 2
Gene namesi
Name:F2RL2
Synonyms:PAR3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000164220.6
HGNCiHGNC:3539 F2RL2
MIMi601919 gene
neXtProtiNX_O00254

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini39 – 94ExtracellularSequence analysisAdd BLAST56
Transmembranei95 – 120Helical; Name=1Sequence analysisAdd BLAST26
Topological domaini121 – 128CytoplasmicSequence analysis8
Transmembranei129 – 148Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini149 – 167ExtracellularSequence analysisAdd BLAST19
Transmembranei168 – 189Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini190 – 206CytoplasmicSequence analysisAdd BLAST17
Transmembranei207 – 230Helical; Name=4Sequence analysisAdd BLAST24
Topological domaini231 – 260ExtracellularSequence analysisAdd BLAST30
Transmembranei261 – 280Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini281 – 297CytoplasmicSequence analysisAdd BLAST17
Transmembranei298 – 322Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini323 – 336ExtracellularSequence analysisAdd BLAST14
Transmembranei337 – 361Helical; Name=7Sequence analysisAdd BLAST25
Topological domaini362 – 374CytoplasmicSequence analysisAdd BLAST13

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39T → P: No proteolytic cleavage by thrombin. 1 Publication1
Mutagenesisi40F → A: Altered signal upon thrombin cleavage. 1 Publication1

Organism-specific databases

DisGeNETi2151
OpenTargetsiENSG00000164220
PharmGKBiPA27948

Chemistry databases

ChEMBLiCHEMBL5477

Polymorphism and mutation databases

BioMutaiF2RL2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000001275622 – 38Removed for receptor activationBy similarityAdd BLAST17
ChainiPRO_000001275739 – 374Proteinase-activated receptor 3Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi166 ↔ 245PROSITE-ProRule annotation
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38 – 39Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO00254
PRIDEiO00254
ProteomicsDBi47807

PTM databases

iPTMnetiO00254
PhosphoSitePlusiO00254

Miscellaneous databases

PMAP-CutDBiO00254

Expressioni

Tissue specificityi

Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.1 Publication

Gene expression databases

BgeeiENSG00000164220
CleanExiHS_F2RL2
GenevisibleiO00254 HS

Interactioni

Subunit structurei

Interacts with INSC/inscuteable and probably GPSM2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK1P163332EBI-1751853,EBI-389883

Protein-protein interaction databases

DIPiDIP-44337N
IntActiO00254, 10 interactors
MINTiO00254
STRINGi9606.ENSP00000296641

Structurei

3D structure databases

ProteinModelPortaliO00254
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJUX Eukaryota
ENOG4111GWA LUCA
GeneTreeiENSGT00920000148955
HOGENOMiHOG000116291
HOVERGENiHBG105658
InParanoidiO00254
KOiK04235
OMAiSAIEGWT
OrthoDBiEOG091G0C2F
PhylomeDBiO00254
TreeFamiTF330775

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR003943 Prot_act_rcpt_3
IPR003912 Protea_act_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR01428 PROTEASEAR
PR01429 PROTEASEAR3
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKALIFAAAG LLLLLPTFCQ SGMENDTNNL AKPTLPIKTF RGAPPNSFEE
60 70 80 90 100
FPFSALEGWT GATITVKIKC PEESASHLHV KNATMGYLTS SLSTKLIPAI
110 120 130 140 150
YLLVFVVGVP ANAVTLWMLF FRTRSICTTV FYTNLAIADF LFCVTLPFKI
160 170 180 190 200
AYHLNGNNWV FGEVLCRATT VIFYGNMYCS ILLLACISIN RYLAIVHPFT
210 220 230 240 250
YRGLPKHTYA LVTCGLVWAT VFLYMLPFFI LKQEYYLVQP DITTCHDVHN
260 270 280 290 300
TCESSSPFQL YYFISLAFFG FLIPFVLIIY CYAAIIRTLN AYDHRWLWYV
310 320 330 340 350
KASLLILVIF TICFAPSNII LIIHHANYYY NNTDGLYFIY LIALCLGSLN
360 370
SCLDPFLYFL MSKTRNHSTA YLTK
Length:374
Mass (Da):42,508
Last modified:July 1, 1997 - v1
Checksum:iC45C15A695DD1ABB
GO
Isoform 2 (identifier: O00254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:352
Mass (Da):40,247
Checksum:i36F6931ABB5C5393
GO

Sequence cautioni

The sequence CAD97628 differs from that shown. Reason: Frameshift at position 374.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01284915L → S1 PublicationCorresponds to variant dbSNP:rs2069649Ensembl.1
Natural variantiVAR_012850177M → V1 PublicationCorresponds to variant dbSNP:rs2069700Ensembl.1
Natural variantiVAR_012851250N → D1 PublicationCorresponds to variant dbSNP:rs2069683Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0451161 – 22Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92971 mRNA Translation: AAC51218.1
AK312848 mRNA Translation: BAG35701.1
AK298585 mRNA Translation: BAG60775.1
BX537386 mRNA Translation: CAD97628.1 Frameshift.
AF374726 Genomic DNA Translation: AAK51564.1
AC026725 Genomic DNA No translation available.
CH471084 Genomic DNA Translation: EAW95778.1
BC093648 mRNA Translation: AAH93648.1
BC093650 mRNA Translation: AAH93650.1
CCDSiCCDS4031.1 [O00254-1]
CCDS58959.1 [O00254-2]
RefSeqiNP_001243495.1, NM_001256566.1 [O00254-2]
NP_004092.1, NM_004101.3 [O00254-1]
UniGeneiHs.42502

Genome annotation databases

EnsembliENST00000296641; ENSP00000296641; ENSG00000164220 [O00254-1]
ENST00000504899; ENSP00000426703; ENSG00000164220 [O00254-2]
GeneIDi2151
KEGGihsa:2151
UCSCiuc003kem.4 human [O00254-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPAR3_HUMAN
AccessioniPrimary (citable) accession number: O00254
Secondary accession number(s): B2R754
, B4DQ13, Q52M68, Q7Z3W3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: June 20, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

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