UniProtKB - O00233 (PSMD9_HUMAN)
26S proteasome non-ATPase regulatory subunit 9
PSMD9
Functioni
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
1 PublicationCaution
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- transcription coactivator activity Source: BHF-UCL
GO - Biological processi
- negative regulation of insulin secretion Source: BHF-UCL
- positive regulation of insulin secretion Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: BHF-UCL
- proteasome regulatory particle assembly Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Enzyme and pathway databases
PathwayCommonsi | O00233 |
Reactomei | R-HSA-1169091, Activation of NF-kappaB in B cells R-HSA-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1236974, ER-Phagosome pathway R-HSA-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-HSA-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174113, SCF-beta-TrCP mediated degradation of Emi1 R-HSA-174154, APC/C:Cdc20 mediated degradation of Securin R-HSA-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-180534, Vpu mediated degradation of CD4 R-HSA-180585, Vif-mediated degradation of APOBEC3G R-HSA-187577, SCF(Skp2)-mediated degradation of p27/p21 R-HSA-195253, Degradation of beta-catenin by the destruction complex R-HSA-202424, Downstream TCR signaling R-HSA-211733, Regulation of activated PAK-2p34 by proteasome mediated degradation R-HSA-2467813, Separation of Sister Chromatids R-HSA-2871837, FCERI mediated NF-kB activation R-HSA-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-HSA-350562, Regulation of ornithine decarboxylase (ODC) R-HSA-382556, ABC-family proteins mediated transport R-HSA-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-HSA-4608870, Asymmetric localization of PCP proteins R-HSA-4641257, Degradation of AXIN R-HSA-4641258, Degradation of DVL R-HSA-5358346, Hedgehog ligand biogenesis R-HSA-5362768, Hh mutants are degraded by ERAD R-HSA-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5607764, CLEC7A (Dectin-1) signaling R-HSA-5610780, Degradation of GLI1 by the proteasome R-HSA-5610783, Degradation of GLI2 by the proteasome R-HSA-5610785, GLI3 is processed to GLI3R by the proteasome R-HSA-5632684, Hedgehog 'on' state R-HSA-5658442, Regulation of RAS by GAPs R-HSA-5668541, TNFR2 non-canonical NF-kB pathway R-HSA-5676590, NIK-->noncanonical NF-kB signaling R-HSA-5678895, Defective CFTR causes cystic fibrosis R-HSA-5687128, MAPK6/MAPK4 signaling R-HSA-5689603, UCH proteinases R-HSA-5689880, Ub-specific processing proteases R-HSA-68827, CDT1 association with the CDC6:ORC:origin complex R-HSA-68949, Orc1 removal from chromatin R-HSA-69017, CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69481, G2/M Checkpoints R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-75815, Ubiquitin-dependent degradation of Cyclin D R-HSA-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-HSA-8932339, ROS sensing by NFE2L2 R-HSA-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-HSA-8939902, Regulation of RUNX2 expression and activity R-HSA-8941858, Regulation of RUNX3 expression and activity R-HSA-8948751, Regulation of PTEN stability and activity R-HSA-8951664, Neddylation R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9020702, Interleukin-1 signaling R-HSA-9604323, Negative regulation of NOTCH4 signaling R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | O00233 |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome non-ATPase regulatory subunit 9Alternative name(s): 26S proteasome regulatory subunit p27 |
Gene namesi | Name:PSMD9 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9567, PSMD9 |
MIMi | 603146, gene |
neXtProti | NX_O00233 |
VEuPathDBi | HostDB:ENSG00000110801 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: GO_Central
- proteasome regulatory particle Source: UniProtKB
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 5715 |
OpenTargetsi | ENSG00000110801 |
PharmGKBi | PA33913 |
Miscellaneous databases
Pharosi | O00233, Tbio |
Genetic variation databases
BioMutai | PSMD9 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173852 | 1 – 223 | 26S proteasome non-ATPase regulatory subunit 9Add BLAST | 223 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 129 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O00233 |
jPOSTi | O00233 |
MassIVEi | O00233 |
MaxQBi | O00233 |
PaxDbi | O00233 |
PeptideAtlasi | O00233 |
PRIDEi | O00233 |
ProteomicsDBi | 32407 47799 [O00233-1] 47800 [O00233-2] |
TopDownProteomicsi | O00233-1 [O00233-1] |
2D gel databases
OGPi | O00233 |
PTM databases
GlyGeni | O00233, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O00233 |
MetOSitei | O00233 |
PhosphoSitePlusi | O00233 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000110801, Expressed in heart left ventricle and 118 other tissues |
ExpressionAtlasi | O00233, baseline and differential |
Genevisiblei | O00233, HS |
Organism-specific databases
HPAi | ENSG00000110801, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PSMC3.
Part of a transient complex (modulator) containing PSMD9, PSMC6 and PSMC3 formed during the assembly of the 26S proteasome.
1 PublicationBinary interactionsi
O00233
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111687, 121 interactors |
CORUMi | O00233 |
IntActi | O00233, 35 interactors |
MINTi | O00233 |
STRINGi | 9606.ENSP00000440485 |
Miscellaneous databases
RNActi | O00233, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 195 | PDZAdd BLAST | 88 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 103 – 141 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 103 – 123 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 124 – 141 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3129, Eukaryota |
GeneTreei | ENSGT00390000004147 |
HOGENOMi | CLU_073146_3_0_1 |
InParanoidi | O00233 |
OMAi | GCNIVPL |
OrthoDBi | 1405146at2759 |
PhylomeDBi | O00233 |
TreeFami | TF105995 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR040815, Nas2_N IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf IPR035269, PSMD9 |
PANTHERi | PTHR12651, PTHR12651, 1 hit |
Pfami | View protein in Pfam PF18265, Nas2_N, 1 hit PF17820, PDZ_6, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSDEEARQSG GSSQAGVVTV SDVQELMRRK EEIEAQIKAN YDVLESQKGI
60 70 80 90 100
GMNEPLVDCE GYPRSDVDLY QVRTARHNII CLQNDHKAVM KQVEEALHQL
110 120 130 140 150
HARDKEKQAR DMAEAHKEAM SRKLGQSESQ GPPRAFAKVN SISPGSPASI
160 170 180 190 200
AGLQVDDEIV EFGSVNTQNF QSLHNIGSVV QHSEGKPLNV TVIRRGEKHQ
210 220
LRLVPTRWAG KGLLGCNIIP LQR
The sequence of this isoform differs from the canonical sequence as follows:
186-223: KPLNVTVIRRGEKHQLRLVPTRWAGKGLLGCNIIPLQR → ALAPTILLSVSMNLTTPGTSSRSP
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KN29 | J3KN29_HUMAN | 26S proteasome non-ATPase regulator... | PSMD9 | 222 | Annotation score: | ||
F5H169 | F5H169_HUMAN | 26S proteasome non-ATPase regulator... | PSMD9 | 128 | Annotation score: | ||
F5H5V4 | F5H5V4_HUMAN | 26S proteasome non-ATPase regulator... | PSMD9 | 153 | Annotation score: | ||
F5GX23 | F5GX23_HUMAN | 26S proteasome non-ATPase regulator... | PSMD9 | 179 | Annotation score: | ||
F5H7X1 | F5H7X1_HUMAN | 26S proteasome non-ATPase regulator... | PSMD9 | 85 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_009953 | 17 | V → ACombined sources2 PublicationsCorresponds to variant dbSNP:rs2230681Ensembl. | 1 | |
Natural variantiVAR_057047 | 74 | T → I. Corresponds to variant dbSNP:rs2291116Ensembl. | 1 | |
Natural variantiVAR_057048 | 134 | R → W. Corresponds to variant dbSNP:rs1177573Ensembl. | 1 | |
Natural variantiVAR_057049 | 197 | E → G. Corresponds to variant dbSNP:rs14259Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046004 | 47 – 151 | Missing in isoform 3. 1 PublicationAdd BLAST | 105 | |
Alternative sequenceiVSP_005300 | 186 – 223 | KPLNV…IPLQR → ALAPTILLSVSMNLTTPGTS SRSP in isoform p27-S. 1 PublicationAdd BLAST | 38 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB003177 mRNA Translation: BAA19790.1 AK315389 mRNA Translation: BAG37782.1 CB111716 mRNA No translation available. AC069503 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW98296.1 BC002383 mRNA Translation: AAH02383.1 BC004184 mRNA Translation: AAH04184.1 BC004213 mRNA Translation: AAH04213.1 |
CCDSi | CCDS58284.1 [O00233-3] CCDS9225.1 [O00233-1] |
RefSeqi | NP_001248329.1, NM_001261400.2 [O00233-3] NP_002804.2, NM_002813.6 [O00233-1] |
Genome annotation databases
Ensembli | ENST00000537407.5; ENSP00000445058.1; ENSG00000110801.14 [O00233-2] ENST00000541212.6; ENSP00000440485.1; ENSG00000110801.14 ENST00000542602.1; ENSP00000443772.1; ENSG00000110801.14 [O00233-3] |
GeneIDi | 5715 |
KEGGi | hsa:5715 |
MANE-Selecti | ENST00000541212.6; ENSP00000440485.1; NM_002813.7; NP_002804.2 |
UCSCi | uc001ubl.5, human [O00233-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB003177 mRNA Translation: BAA19790.1 AK315389 mRNA Translation: BAG37782.1 CB111716 mRNA No translation available. AC069503 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW98296.1 BC002383 mRNA Translation: AAH02383.1 BC004184 mRNA Translation: AAH04184.1 BC004213 mRNA Translation: AAH04213.1 |
CCDSi | CCDS58284.1 [O00233-3] CCDS9225.1 [O00233-1] |
RefSeqi | NP_001248329.1, NM_001261400.2 [O00233-3] NP_002804.2, NM_002813.6 [O00233-1] |
3D structure databases
AlphaFoldDBi | O00233 |
SMRi | O00233 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111687, 121 interactors |
CORUMi | O00233 |
IntActi | O00233, 35 interactors |
MINTi | O00233 |
STRINGi | 9606.ENSP00000440485 |
PTM databases
GlyGeni | O00233, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O00233 |
MetOSitei | O00233 |
PhosphoSitePlusi | O00233 |
Genetic variation databases
BioMutai | PSMD9 |
2D gel databases
OGPi | O00233 |
Proteomic databases
EPDi | O00233 |
jPOSTi | O00233 |
MassIVEi | O00233 |
MaxQBi | O00233 |
PaxDbi | O00233 |
PeptideAtlasi | O00233 |
PRIDEi | O00233 |
ProteomicsDBi | 32407 47799 [O00233-1] 47800 [O00233-2] |
TopDownProteomicsi | O00233-1 [O00233-1] |
Protocols and materials databases
Antibodypediai | 31601, 286 antibodies from 29 providers |
DNASUi | 5715 |
Genome annotation databases
Ensembli | ENST00000537407.5; ENSP00000445058.1; ENSG00000110801.14 [O00233-2] ENST00000541212.6; ENSP00000440485.1; ENSG00000110801.14 ENST00000542602.1; ENSP00000443772.1; ENSG00000110801.14 [O00233-3] |
GeneIDi | 5715 |
KEGGi | hsa:5715 |
MANE-Selecti | ENST00000541212.6; ENSP00000440485.1; NM_002813.7; NP_002804.2 |
UCSCi | uc001ubl.5, human [O00233-1] |
Organism-specific databases
CTDi | 5715 |
DisGeNETi | 5715 |
GeneCardsi | PSMD9 |
HGNCi | HGNC:9567, PSMD9 |
HPAi | ENSG00000110801, Low tissue specificity |
MIMi | 603146, gene |
neXtProti | NX_O00233 |
OpenTargetsi | ENSG00000110801 |
PharmGKBi | PA33913 |
VEuPathDBi | HostDB:ENSG00000110801 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3129, Eukaryota |
GeneTreei | ENSGT00390000004147 |
HOGENOMi | CLU_073146_3_0_1 |
InParanoidi | O00233 |
OMAi | GCNIVPL |
OrthoDBi | 1405146at2759 |
PhylomeDBi | O00233 |
TreeFami | TF105995 |
Enzyme and pathway databases
PathwayCommonsi | O00233 |
Reactomei | R-HSA-1169091, Activation of NF-kappaB in B cells R-HSA-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1236974, ER-Phagosome pathway R-HSA-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-HSA-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174113, SCF-beta-TrCP mediated degradation of Emi1 R-HSA-174154, APC/C:Cdc20 mediated degradation of Securin R-HSA-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-180534, Vpu mediated degradation of CD4 R-HSA-180585, Vif-mediated degradation of APOBEC3G R-HSA-187577, SCF(Skp2)-mediated degradation of p27/p21 R-HSA-195253, Degradation of beta-catenin by the destruction complex R-HSA-202424, Downstream TCR signaling R-HSA-211733, Regulation of activated PAK-2p34 by proteasome mediated degradation R-HSA-2467813, Separation of Sister Chromatids R-HSA-2871837, FCERI mediated NF-kB activation R-HSA-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-HSA-350562, Regulation of ornithine decarboxylase (ODC) R-HSA-382556, ABC-family proteins mediated transport R-HSA-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-HSA-4608870, Asymmetric localization of PCP proteins R-HSA-4641257, Degradation of AXIN R-HSA-4641258, Degradation of DVL R-HSA-5358346, Hedgehog ligand biogenesis R-HSA-5362768, Hh mutants are degraded by ERAD R-HSA-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5607764, CLEC7A (Dectin-1) signaling R-HSA-5610780, Degradation of GLI1 by the proteasome R-HSA-5610783, Degradation of GLI2 by the proteasome R-HSA-5610785, GLI3 is processed to GLI3R by the proteasome R-HSA-5632684, Hedgehog 'on' state R-HSA-5658442, Regulation of RAS by GAPs R-HSA-5668541, TNFR2 non-canonical NF-kB pathway R-HSA-5676590, NIK-->noncanonical NF-kB signaling R-HSA-5678895, Defective CFTR causes cystic fibrosis R-HSA-5687128, MAPK6/MAPK4 signaling R-HSA-5689603, UCH proteinases R-HSA-5689880, Ub-specific processing proteases R-HSA-68827, CDT1 association with the CDC6:ORC:origin complex R-HSA-68949, Orc1 removal from chromatin R-HSA-69017, CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69481, G2/M Checkpoints R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-75815, Ubiquitin-dependent degradation of Cyclin D R-HSA-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-HSA-8932339, ROS sensing by NFE2L2 R-HSA-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-HSA-8939902, Regulation of RUNX2 expression and activity R-HSA-8941858, Regulation of RUNX3 expression and activity R-HSA-8948751, Regulation of PTEN stability and activity R-HSA-8951664, Neddylation R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9020702, Interleukin-1 signaling R-HSA-9604323, Negative regulation of NOTCH4 signaling R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | O00233 |
Miscellaneous databases
BioGRID-ORCSi | 5715, 74 hits in 1078 CRISPR screens |
ChiTaRSi | PSMD9, human |
GeneWikii | PSMD9 |
GenomeRNAii | 5715 |
Pharosi | O00233, Tbio |
PROi | PR:O00233 |
RNActi | O00233, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000110801, Expressed in heart left ventricle and 118 other tissues |
ExpressionAtlasi | O00233, baseline and differential |
Genevisiblei | O00233, HS |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR040815, Nas2_N IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf IPR035269, PSMD9 |
PANTHERi | PTHR12651, PTHR12651, 1 hit |
Pfami | View protein in Pfam PF18265, Nas2_N, 1 hit PF17820, PDZ_6, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PSMD9_HUMAN | |
Accessioni | O00233Primary (citable) accession number: O00233 Secondary accession number(s): B2RD35, G3V1Q6, Q9BQ42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 11, 2011 | |
Last modified: | May 25, 2022 | |
This is version 199 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families