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Protein

NF-kappa-B inhibitor epsilon

Gene

NFKBIE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.1 Publication

GO - Biological processi

  • cytoplasmic sequestering of transcription factor Source: ProtInc
  • D-serine transport Source: Ensembl

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
SIGNORiO00221

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor epsilon
Short name:
NF-kappa-BIE
Alternative name(s):
I-kappa-B-epsilon
Short name:
IkB-E
Short name:
IkB-epsilon
Short name:
IkappaBepsilon
Gene namesi
Name:NFKBIE
Synonyms:IKBE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000146232.14
HGNCiHGNC:7799 NFKBIE
MIMi604548 gene
neXtProtiNX_O00221

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi145K → R: No effect. 1 Publication1
Mutagenesisi157S → A: No degradation. 1 Publication1
Mutagenesisi161S → A: No degradation. 1 Publication1

Organism-specific databases

DisGeNETi4794
OpenTargetsiENSG00000146232
PharmGKBiPA31603

Polymorphism and mutation databases

BioMutaiNFKBIE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670071 – 500NF-kappa-B inhibitor epsilonAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Modified residuei161PhosphoserineCurated1
Modified residuei183PhosphoserineCombined sources1

Post-translational modificationi

Serine phosphorylated; followed by proteasome-dependent degradation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO00221
MaxQBiO00221
PaxDbiO00221
PeptideAtlasiO00221
PRIDEiO00221
ProteomicsDBi47791

PTM databases

iPTMnetiO00221
PhosphoSitePlusiO00221

Expressioni

Tissue specificityi

Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.

Gene expression databases

BgeeiENSG00000146232 Expressed in 139 organ(s), highest expression level in testis
CleanExiHS_NFKBIE
ExpressionAtlasiO00221 baseline and differential
GenevisibleiO00221 HS

Organism-specific databases

HPAiCAB010272
HPA002692
HPA005941

Interactioni

Subunit structurei

Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi110861, 25 interactors
CORUMiO00221
DIPiDIP-27533N
ELMiO00221
IntActiO00221, 24 interactors
STRINGi9606.ENSP00000275015

Structurei

3D structure databases

ProteinModelPortaliO00221
SMRiO00221
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati258 – 291ANK 1Add BLAST34
Repeati293 – 322ANK 2Add BLAST30
Repeati326 – 355ANK 3Add BLAST30
Repeati369 – 398ANK 4Add BLAST30
Repeati403 – 432ANK 5Add BLAST30
Repeati436 – 465ANK 6Add BLAST30

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00550000074527
HOGENOMiHOG000059576
HOVERGENiHBG108320
InParanoidiO00221
KOiK05872
OMAiNMEDETA
OrthoDBiEOG091G092R
PhylomeDBiO00221
TreeFamiTF320166

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O00221-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR
60 70 80 90 100
PCWSGGAGRG GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR
110 120 130 140 150
PPRAGRPLCQ ADPQPGKAAR RSLEPDPAQT GPRPARAAGM SEARKGPDEA
160 170 180 190 200
EESQYDSGIE SLRSLRSLPE STSAPASGPS DGSPQPCTHP PGPVKEPQEK
210 220 230 240 250
EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH VGALSPQQLE
260 270 280 290 300
ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL
310 320 330 340 350
AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR
360 370 380 390 400
PEPGRGTSHS LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE
410 420 430 440 450
GTSGKTALHL AVETQERGLV QFLLQAGAQV DARMLNGCTP LHLAAGRGLM
460 470 480 490 500
GISSTLCKAG ADSLLRNVED ETPQDLTEES LVLLPFDDLK ISGKLLLCTD
Length:500
Mass (Da):52,864
Last modified:September 23, 2008 - v3
Checksum:i45E726E7A0E8D478
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q7LC14Q7LC14_HUMAN
NFKBIE protein
NFKBIE
361Annotation score:
H0Y4W4H0Y4W4_HUMAN
NF-kappa-B inhibitor epsilon
NFKBIE
55Annotation score:
H3BNC2H3BNC2_HUMAN
NF-kappa-B inhibitor epsilon
NFKBIE
141Annotation score:

Sequence cautioni

The sequence AAC51216 differs from that shown. Reason: Frameshift at positions 339 and 351.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63 – 64PA → RP in AAC51216 (PubMed:9135156).Curated2
Sequence conflicti101P → R in AAC51216 (PubMed:9135156).Curated1
Sequence conflicti233A → P in AAC51216 (PubMed:9135156).Curated1
Sequence conflicti402T → S in AAC51216 (PubMed:9135156).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04663195H → Q. Corresponds to variant dbSNP:rs28362857Ensembl.1
Natural variantiVAR_046632194V → A. Corresponds to variant dbSNP:rs2233434Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91616 mRNA Translation: AAC51216.1 Frameshift.
AL139392 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04262.1
CCDSiCCDS34463.1
RefSeqiNP_004547.2, NM_004556.2
UniGeneiHs.458276

Genome annotation databases

EnsembliENST00000275015; ENSP00000275015; ENSG00000146232
GeneIDi4794
KEGGihsa:4794
UCSCiuc003oxe.1 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91616 mRNA Translation: AAC51216.1 Frameshift.
AL139392 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04262.1
CCDSiCCDS34463.1
RefSeqiNP_004547.2, NM_004556.2
UniGeneiHs.458276

3D structure databases

ProteinModelPortaliO00221
SMRiO00221
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110861, 25 interactors
CORUMiO00221
DIPiDIP-27533N
ELMiO00221
IntActiO00221, 24 interactors
STRINGi9606.ENSP00000275015

PTM databases

iPTMnetiO00221
PhosphoSitePlusiO00221

Polymorphism and mutation databases

BioMutaiNFKBIE

Proteomic databases

EPDiO00221
MaxQBiO00221
PaxDbiO00221
PeptideAtlasiO00221
PRIDEiO00221
ProteomicsDBi47791

Protocols and materials databases

DNASUi4794
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000275015; ENSP00000275015; ENSG00000146232
GeneIDi4794
KEGGihsa:4794
UCSCiuc003oxe.1 human

Organism-specific databases

CTDi4794
DisGeNETi4794
EuPathDBiHostDB:ENSG00000146232.14
GeneCardsiNFKBIE
H-InvDBiHIX0200879
HGNCiHGNC:7799 NFKBIE
HPAiCAB010272
HPA002692
HPA005941
MIMi604548 gene
neXtProtiNX_O00221
OpenTargetsiENSG00000146232
PharmGKBiPA31603
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00550000074527
HOGENOMiHOG000059576
HOVERGENiHBG108320
InParanoidiO00221
KOiK05872
OMAiNMEDETA
OrthoDBiEOG091G092R
PhylomeDBiO00221
TreeFamiTF320166

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
SIGNORiO00221

Miscellaneous databases

ChiTaRSiNFKBIE human
GeneWikiiNFKBIE
GenomeRNAii4794
PROiPR:O00221
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000146232 Expressed in 139 organ(s), highest expression level in testis
CleanExiHS_NFKBIE
ExpressionAtlasiO00221 baseline and differential
GenevisibleiO00221 HS

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiIKBE_HUMAN
AccessioniPrimary (citable) accession number: O00221
Secondary accession number(s): Q5T9V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: September 23, 2008
Last modified: September 12, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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