UniProtKB - O00219 (HYAS3_HUMAN)
Hyaluronan synthase 3
HAS3
Functioni
Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction (By similarity).
By similarityCatalytic activityi
- [hyaluronan](n) + UDP-N-acetyl-α-D-glucosamine = H+ + N-acetyl-β-D-glucosaminyl-(1→4)-[hyaluronan](n) + UDPEC:2.4.1.212
- N-acetyl-β-D-glucosaminyl-(1→4)-[hyaluronan](n) + UDP-α-D-glucuronate = [hyaluronan](n+1) + H+ + UDPEC:2.4.1.212
Cofactori
: hyaluronan biosynthesis Pathwayi
This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.
GO - Molecular functioni
- hyaluronan synthase activity Source: UniProtKB
- identical protein binding Source: IntAct
GO - Biological processi
- carbohydrate metabolic process Source: ProtInc
- extracellular matrix assembly Source: UniProtKB
- extracellular polysaccharide biosynthetic process Source: UniProtKB
- hyaluronan biosynthetic process Source: UniProtKB
- positive regulation of hyaluranon cable assembly Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.212, 2681 |
PathwayCommonsi | O00219 |
Reactomei | R-HSA-2142850, Hyaluronan biosynthesis and export |
SignaLinki | O00219 |
UniPathwayi | UPA00341 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
Names & Taxonomyi
Protein namesi | Recommended name: Hyaluronan synthase 3 (EC:2.4.1.212)Alternative name(s): Hyaluronate synthase 3 Hyaluronic acid synthase 3 Short name: HA synthase 3 |
Gene namesi | Name:HAS3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4820, HAS3 |
MIMi | 602428, gene |
neXtProti | NX_O00219 |
VEuPathDBi | HostDB:ENSG00000103044 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Extracellular region or secreted
- hyaluranon cable Source: UniProtKB
Nucleus
- nucleus Source: Ensembl
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: Reactome
Other locations
- cytoplasm Source: Ensembl
- integral component of membrane Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 16 – 36 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 44 | ExtracellularSequence analysis | 8 | |
Transmembranei | 45 – 65 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 66 – 377 | CytoplasmicSequence analysisAdd BLAST | 312 | |
Transmembranei | 378 – 398 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 399 – 408 | ExtracellularSequence analysis | 10 | |
Transmembranei | 409 – 429 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 430 – 440 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 441 – 461 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 462 – 473 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 474 – 494 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 495 – 515 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 516 – 536 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 537 – 553 | ExtracellularSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 3038 |
OpenTargetsi | ENSG00000103044 |
PharmGKBi | PA29196 |
Miscellaneous databases
Pharosi | O00219, Tbio |
Genetic variation databases
BioMutai | HAS3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197178 | 1 – 553 | Hyaluronan synthase 3Add BLAST | 553 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 462 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | O00219 |
MassIVEi | O00219 |
MaxQBi | O00219 |
PaxDbi | O00219 |
PeptideAtlasi | O00219 |
PRIDEi | O00219 |
ProteomicsDBi | 47788 [O00219-1] 47789 [O00219-2] |
PTM databases
GlyGeni | O00219, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | O00219 |
PhosphoSitePlusi | O00219 |
Expressioni
Gene expression databases
Bgeei | ENSG00000103044, Expressed in secondary oocyte and 171 other tissues |
ExpressionAtlasi | O00219, baseline and differential |
Genevisiblei | O00219, HS |
Organism-specific databases
HPAi | ENSG00000103044, Tissue enhanced (esophagus, urinary bladder) |
Interactioni
Binary interactionsi
O00219
With | #Exp. | IntAct |
---|---|---|
HAS1 [Q92839] | 9 | EBI-16628799,EBI-1052423 |
HAS2 [Q92819] | 10 | EBI-16628799,EBI-16628852 |
itself | 10 | EBI-16628799,EBI-16628799 |
Isoform 2 [O00219-2]
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 109288, 11 interactors |
CORUMi | O00219 |
IntActi | O00219, 9 interactors |
STRINGi | 9606.ENSP00000304440 |
Miscellaneous databases
RNActi | O00219, protein |
Structurei
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2571, Eukaryota |
GeneTreei | ENSGT00390000010337 |
HOGENOMi | CLU_029695_3_0_1 |
InParanoidi | O00219 |
OMAi | DVIVPCF |
PhylomeDBi | O00219 |
TreeFami | TF332506 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR029044, Nucleotide-diphossugar_trans |
SUPFAMi | SSF53448, SSF53448, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVQLTTALR VVGTSLFALA VLGGILAAYV TGYQFIHTEK HYLSFGLYGA
60 70 80 90 100
ILGLHLLIQS LFAFLEHRRM RRAGQALKLP SPRRGSVALC IAAYQEDPDY
110 120 130 140 150
LRKCLRSAQR ISFPDLKVVM VVDGNRQEDA YMLDIFHEVL GGTEQAGFFV
160 170 180 190 200
WRSNFHEAGE GETEASLQEG MDRVRDVVRA STFSCIMQKW GGKREVMYTA
210 220 230 240 250
FKALGDSVDY IQVCDSDTVL DPACTIEMLR VLEEDPQVGG VGGDVQILNK
260 270 280 290 300
YDSWISFLSS VRYWMAFNVE RACQSYFGCV QCISGPLGMY RNSLLQQFLE
310 320 330 340 350
DWYHQKFLGS KCSFGDDRHL TNRVLSLGYR TKYTARSKCL TETPTKYLRW
360 370 380 390 400
LNQQTRWSKS YFREWLYNSL WFHKHHLWMT YESVVTGFFP FFLIATVIQL
410 420 430 440 450
FYRGRIWNIL LFLLTVQLVG IIKATYACFL RGNAEMIFMS LYSLLYMSSL
460 470 480 490 500
LPAKIFAIAT INKSGWGTSG RKTIVVNFIG LIPVSIWVAV LLGGLAYTAY
510 520 530 540 550
CQDLFSETEL AFLVSGAILY GCYWVALLML YLAIIARRCG KKPEQYSLAF
AEV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH3BRH5 | H3BRH5_HUMAN | Hyaluronan synthase 3 | HAS3 | 264 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 493 | G → E in AAF36984 (Ref. 1) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049317 | 173 | R → H. Corresponds to variant dbSNP:rs2232229Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042022 | 247 – 553 | ILNKY…AFAEV → PPGKGMAVEDDQVQAAQVRA TEAWSVHQRHVSREQ in isoform 2. 1 PublicationAdd BLAST | 307 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF232772 mRNA Translation: AAF36984.1 AK291400 mRNA Translation: BAF84089.1 AC009027 Genomic DNA No translation available. CH471092 Genomic DNA Translation: EAW83247.1 CH471092 Genomic DNA Translation: EAW83248.1 BC021853 mRNA Translation: AAH21853.1 U86409 Genomic DNA Translation: AAC51209.1 |
CCDSi | CCDS10870.1 [O00219-2] CCDS10871.1 [O00219-1] |
RefSeqi | NP_001186209.1, NM_001199280.1 [O00219-1] NP_005320.2, NM_005329.2 [O00219-1] NP_619515.1, NM_138612.2 [O00219-2] XP_005255978.1, XM_005255921.2 [O00219-1] XP_011521363.1, XM_011523061.2 [O00219-1] |
Genome annotation databases
Ensembli | ENST00000219322; ENSP00000219322; ENSG00000103044 [O00219-2] ENST00000306560; ENSP00000304440; ENSG00000103044 ENST00000569188; ENSP00000454731; ENSG00000103044 |
GeneIDi | 3038 |
KEGGi | hsa:3038 |
MANE-Selecti | ENST00000569188.6; ENSP00000454731.1; NM_001199280.2; NP_001186209.1 |
UCSCi | uc002ewk.4, human [O00219-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF232772 mRNA Translation: AAF36984.1 AK291400 mRNA Translation: BAF84089.1 AC009027 Genomic DNA No translation available. CH471092 Genomic DNA Translation: EAW83247.1 CH471092 Genomic DNA Translation: EAW83248.1 BC021853 mRNA Translation: AAH21853.1 U86409 Genomic DNA Translation: AAC51209.1 |
CCDSi | CCDS10870.1 [O00219-2] CCDS10871.1 [O00219-1] |
RefSeqi | NP_001186209.1, NM_001199280.1 [O00219-1] NP_005320.2, NM_005329.2 [O00219-1] NP_619515.1, NM_138612.2 [O00219-2] XP_005255978.1, XM_005255921.2 [O00219-1] XP_011521363.1, XM_011523061.2 [O00219-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 109288, 11 interactors |
CORUMi | O00219 |
IntActi | O00219, 9 interactors |
STRINGi | 9606.ENSP00000304440 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
PTM databases
GlyGeni | O00219, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | O00219 |
PhosphoSitePlusi | O00219 |
Genetic variation databases
BioMutai | HAS3 |
Proteomic databases
EPDi | O00219 |
MassIVEi | O00219 |
MaxQBi | O00219 |
PaxDbi | O00219 |
PeptideAtlasi | O00219 |
PRIDEi | O00219 |
ProteomicsDBi | 47788 [O00219-1] 47789 [O00219-2] |
Protocols and materials databases
Antibodypediai | 29818, 266 antibodies from 32 providers |
DNASUi | 3038 |
Genome annotation databases
Ensembli | ENST00000219322; ENSP00000219322; ENSG00000103044 [O00219-2] ENST00000306560; ENSP00000304440; ENSG00000103044 ENST00000569188; ENSP00000454731; ENSG00000103044 |
GeneIDi | 3038 |
KEGGi | hsa:3038 |
MANE-Selecti | ENST00000569188.6; ENSP00000454731.1; NM_001199280.2; NP_001186209.1 |
UCSCi | uc002ewk.4, human [O00219-1] |
Organism-specific databases
CTDi | 3038 |
DisGeNETi | 3038 |
GeneCardsi | HAS3 |
HGNCi | HGNC:4820, HAS3 |
HPAi | ENSG00000103044, Tissue enhanced (esophagus, urinary bladder) |
MIMi | 602428, gene |
neXtProti | NX_O00219 |
OpenTargetsi | ENSG00000103044 |
PharmGKBi | PA29196 |
VEuPathDBi | HostDB:ENSG00000103044 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2571, Eukaryota |
GeneTreei | ENSGT00390000010337 |
HOGENOMi | CLU_029695_3_0_1 |
InParanoidi | O00219 |
OMAi | DVIVPCF |
PhylomeDBi | O00219 |
TreeFami | TF332506 |
Enzyme and pathway databases
UniPathwayi | UPA00341 |
BRENDAi | 2.4.1.212, 2681 |
PathwayCommonsi | O00219 |
Reactomei | R-HSA-2142850, Hyaluronan biosynthesis and export |
SignaLinki | O00219 |
Miscellaneous databases
BioGRID-ORCSi | 3038, 9 hits in 1037 CRISPR screens |
ChiTaRSi | HAS3, human |
GeneWikii | HAS3 |
GenomeRNAii | 3038 |
Pharosi | O00219, Tbio |
PROi | PR:O00219 |
RNActi | O00219, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103044, Expressed in secondary oocyte and 171 other tissues |
ExpressionAtlasi | O00219, baseline and differential |
Genevisiblei | O00219, HS |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR029044, Nucleotide-diphossugar_trans |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HYAS3_HUMAN | |
Accessioni | O00219Primary (citable) accession number: O00219 Secondary accession number(s): A8K5T5, Q8WTZ0, Q9NYP0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | September 23, 2008 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families