UniProtKB - O00168 (PLM_HUMAN)
Phospholemman
FXYD1
Functioni
GO - Molecular functioni
- chloride channel activity Source: ProtInc
- ion channel binding Source: BHF-UCL
- ion channel regulator activity Source: InterPro
- sodium channel regulator activity Source: BHF-UCL
GO - Biological processi
- chloride transport Source: ProtInc
- ion transmembrane transport Source: Reactome
- muscle contraction Source: ProtInc
- negative regulation of protein glutathionylation Source: UniProtKB
- positive regulation of sodium ion export across plasma membrane Source: UniProtKB
- potassium ion transport Source: UniProtKB-KW
- regulation of cardiac conduction Source: Reactome
- regulation of cardiac muscle cell membrane potential Source: BHF-UCL
- regulation of heart contraction Source: BHF-UCL
- regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
- sodium ion transport Source: UniProtKB-KW
Keywordsi
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | Potassium, Sodium |
Enzyme and pathway databases
PathwayCommonsi | O00168 |
Reactomei | R-HSA-5578775, Ion homeostasis R-HSA-936837, Ion transport by P-type ATPases R-HSA-9679191, Potential therapeutics for SARS |
SIGNORi | O00168 |
Protein family/group databases
TCDBi | 1.A.27.1.8, the phospholemman (plm) family |
Names & Taxonomyi
Protein namesi | Recommended name: PhospholemmanBy similarityAlternative name(s): FXYD domain-containing ion transport regulator 1Imported Sodium/potassium-transporting ATPase subunit FXYD1Curated |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4025, FXYD1 |
MIMi | 602359, gene |
neXtProti | NX_O00168 |
VEuPathDBi | HostDB:ENSG00000266964.5 |
Subcellular locationi
Plasma membrane
- sarcolemma By similarity; Single-pass type I membrane protein Sequence analysis
- Apical cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
- T-tubule By similarity
Other locations
- caveola By similarity
Note: Detected in the apical cell membrane in brain. In myocytes, localizes to sarcolemma, t-tubules and intercalated disks.By similarity
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- caveola Source: UniProtKB
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: Reactome
- sarcolemma Source: UniProtKB
- sodium:potassium-exchanging ATPase complex Source: UniProtKB
- T-tubule Source: UniProtKB
Other locations
- intercalated disc Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 35 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 36 – 56 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 57 – 92 | CytoplasmicSequence analysisAdd BLAST | 36 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 60 | C → S: Significantly reduced half-life; when associated with S-62. 1 Publication | 1 | |
Mutagenesisi | 62 | C → S: Significantly reduced half-life; when associated with S-60. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5348 |
OpenTargetsi | ENSG00000266964 |
PharmGKBi | PA28441 |
Miscellaneous databases
Pharosi | O00168, Tbio |
Genetic variation databases
BioMutai | FXYD1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | By similarityAdd BLAST | 20 | |
ChainiPRO_0000010359 | 21 – 92 | PhospholemmanAdd BLAST | 72 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 60 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 62 | S-glutathionyl cysteine; alternateBy similarity | 1 | |
Lipidationi | 62 | S-palmitoyl cysteine; alternate1 Publication | 1 | |
Modified residuei | 79 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 82 | PhosphoserineBy similarity | 1 | |
Modified residuei | 83 | Phosphoserine; by PKA and PKCBy similarity | 1 | |
Modified residuei | 88 | Phosphoserine; by PKA1 Publication | 1 | |
Modified residuei | 89 | Phosphothreonine; by PKCBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glutathionylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
jPOSTi | O00168 |
MassIVEi | O00168 |
MaxQBi | O00168 |
PaxDbi | O00168 |
PeptideAtlasi | O00168 |
PRIDEi | O00168 |
ProteomicsDBi | 47759 |
PTM databases
iPTMneti | O00168 |
PhosphoSitePlusi | O00168 |
SwissPalmi | O00168 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000266964, Expressed in brain and 115 other tissues |
ExpressionAtlasi | O00168, baseline and differential |
Genevisiblei | O00168, HS |
Organism-specific databases
HPAi | ENSG00000266964, Group enriched (heart muscle, liver, skeletal muscle) |
Interactioni
Subunit structurei
Homotetramer (PubMed:16597826). Monomer (PubMed:17511473). Regulatory subunit of the sodium/potassium-transporting ATPase (NKA) which is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit (By similarity). The monomeric form associates with NKA while the oligomeric form does not (By similarity).
Interacts with the catalytic alpha-1 subunit ATP1A1 (By similarity).
Also interacts with the catalytic alpha-2 and alpha-3 subunits ATP1A2 and ATP1A3 (By similarity). Very little interaction with ATP1A1, ATP1A2 or ATP1A3 when phosphorylated at Ser-83 (By similarity).
Interacts with the non-catalytic beta-1 subunit ATP1B1 (By similarity). Oxidative stress decreases interaction with ATP1A1 but increases interaction with ATP1B1 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- ion channel binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111363, 17 interactors |
IntActi | O00168, 10 interactors |
STRINGi | 9606.ENSP00000481244 |
Miscellaneous databases
RNActi | O00168, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | O00168 |
SMRi | O00168 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O00168 |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S5XM, Eukaryota |
GeneTreei | ENSGT00940000153062 |
HOGENOMi | CLU_171208_2_0_1 |
InParanoidi | O00168 |
OrthoDBi | 1621616at2759 |
PhylomeDBi | O00168 |
TreeFami | TF333443 |
Family and domain databases
InterProi | View protein in InterPro IPR000272, Ion-transport_regulator_FXYD |
Pfami | View protein in Pfam PF02038, ATP1G1_PLM_MAT8, 1 hit |
PROSITEi | View protein in PROSITE PS01310, FXYD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MASLGHILVF CVGLLTMAKA ESPKEHDPFT YDYQSLQIGG LVIAGILFIL
60 70 80 90
GILIVLSRRC RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketK7EQG4 | K7EQG4_HUMAN | FXYD domain-containing ion transpor... | FXYD1 | 115 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | S → P in AAC51286 (PubMed:9169143).Curated | 1 | |
Sequence conflicti | 5 | G → H in AAC51286 (PubMed:9169143).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72245 mRNA Translation: AAC51286.1 AK289811 mRNA Translation: BAF82500.1 BC032800 mRNA Translation: AAH32800.1 |
CCDSi | CCDS12445.1 |
RefSeqi | NP_001265646.1, NM_001278717.1 NP_001265647.1, NM_001278718.1 NP_005022.2, NM_005031.4 NP_068702.1, NM_021902.3 XP_016882363.1, XM_017026874.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72245 mRNA Translation: AAC51286.1 AK289811 mRNA Translation: BAF82500.1 BC032800 mRNA Translation: AAH32800.1 |
CCDSi | CCDS12445.1 |
RefSeqi | NP_001265646.1, NM_001278717.1 NP_001265647.1, NM_001278718.1 NP_005022.2, NM_005031.4 NP_068702.1, NM_021902.3 XP_016882363.1, XM_017026874.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2J1I | model | - | A/B/C/D | 32-59 | [»] | |
2JO1 | NMR | - | A | 21-92 | [»] | |
BMRBi | O00168 | |||||
SMRi | O00168 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111363, 17 interactors |
IntActi | O00168, 10 interactors |
STRINGi | 9606.ENSP00000481244 |
Protein family/group databases
TCDBi | 1.A.27.1.8, the phospholemman (plm) family |
PTM databases
iPTMneti | O00168 |
PhosphoSitePlusi | O00168 |
SwissPalmi | O00168 |
Genetic variation databases
BioMutai | FXYD1 |
Proteomic databases
jPOSTi | O00168 |
MassIVEi | O00168 |
MaxQBi | O00168 |
PaxDbi | O00168 |
PeptideAtlasi | O00168 |
PRIDEi | O00168 |
ProteomicsDBi | 47759 |
Protocols and materials databases
Antibodypediai | 65378, 287 antibodies |
DNASUi | 5348 |
Genome annotation databases
Organism-specific databases
CTDi | 5348 |
DisGeNETi | 5348 |
GeneCardsi | FXYD1 |
HGNCi | HGNC:4025, FXYD1 |
HPAi | ENSG00000266964, Group enriched (heart muscle, liver, skeletal muscle) |
MIMi | 602359, gene |
neXtProti | NX_O00168 |
OpenTargetsi | ENSG00000266964 |
PharmGKBi | PA28441 |
VEuPathDBi | HostDB:ENSG00000266964.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S5XM, Eukaryota |
GeneTreei | ENSGT00940000153062 |
HOGENOMi | CLU_171208_2_0_1 |
InParanoidi | O00168 |
OrthoDBi | 1621616at2759 |
PhylomeDBi | O00168 |
TreeFami | TF333443 |
Enzyme and pathway databases
PathwayCommonsi | O00168 |
Reactomei | R-HSA-5578775, Ion homeostasis R-HSA-936837, Ion transport by P-type ATPases R-HSA-9679191, Potential therapeutics for SARS |
SIGNORi | O00168 |
Miscellaneous databases
BioGRID-ORCSi | 5348, 6 hits in 873 CRISPR screens |
ChiTaRSi | FXYD1, human |
EvolutionaryTracei | O00168 |
GeneWikii | FXYD1 |
GenomeRNAii | 5348 |
Pharosi | O00168, Tbio |
PROi | PR:O00168 |
RNActi | O00168, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000266964, Expressed in brain and 115 other tissues |
ExpressionAtlasi | O00168, baseline and differential |
Genevisiblei | O00168, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000272, Ion-transport_regulator_FXYD |
Pfami | View protein in Pfam PF02038, ATP1G1_PLM_MAT8, 1 hit |
PROSITEi | View protein in PROSITE PS01310, FXYD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLM_HUMAN | |
Accessioni | O00168Primary (citable) accession number: O00168 Secondary accession number(s): A8K196 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | August 30, 2002 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM