UniProtKB - O00161 (SNP23_HUMAN)
Synaptosomal-associated protein 23
SNAP23
Functioni
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- syntaxin binding Source: UniProtKB
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
- exocytosis Source: GO_Central
- histamine secretion by mast cell Source: UniProtKB
- membrane fusion Source: ProtInc
- neutrophil degranulation Source: Reactome
- post-Golgi vesicle-mediated transport Source: Reactome
- protein transport Source: UniProtKB-KW
- synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
- synaptic vesicle priming Source: GO_Central
- vesicle fusion Source: GO_Central
- vesicle targeting Source: ProtInc
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | O00161 |
Reactomei | R-HSA-1236974, ER-Phagosome pathway R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-199992, trans-Golgi Network Vesicle Budding R-HSA-6798695, Neutrophil degranulation |
SIGNORi | O00161 |
Protein family/group databases
TCDBi | 1.F.1.1.1, the synaptosomal vesicle fusion pore (svf-pore) family |
Names & Taxonomyi
Protein namesi | Recommended name: Synaptosomal-associated protein 23Short name: SNAP-23 Alternative name(s): Vesicle-membrane fusion protein SNAP-23 |
Gene namesi | Name:SNAP23 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11131, SNAP23 |
MIMi | 602534, gene |
neXtProti | NX_O00161 |
VEuPathDBi | HostDB:ENSG00000092531.9 |
Subcellular locationi
Plasma membrane
Other locations
Note: Mainly localized to the plasma membrane.
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Lysosome
- azurophil granule Source: UniProtKB
Mitochondrion
- mitochondrion Source: CACAO
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- adherens junction Source: BHF-UCL
- cytoplasm Source: UniProtKB
- focal adhesion Source: UniProtKB
- neuron projection Source: UniProtKB-SubCell
- phagocytic vesicle membrane Source: Reactome
- presynapse Source: GOC
- SNARE complex Source: UniProtKB
- specific granule Source: UniProtKB
- specific granule membrane Source: Reactome
- tertiary granule membrane Source: Reactome
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Synapse, SynaptosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 8773 |
OpenTargetsi | ENSG00000092531 |
PharmGKBi | PA35979 |
Miscellaneous databases
Pharosi | O00161, Tbio |
Genetic variation databases
BioMutai | SNAP23 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213598 | 1 – 211 | Synaptosomal-associated protein 23Add BLAST | 211 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources2 Publications | 1 | |
Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Modified residuei | 34 | PhosphoserineCombined sources | 1 | |
Lipidationi | 79 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 80 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 83 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 85 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 87 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Lipidationi | 112 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 161 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | O00161 |
jPOSTi | O00161 |
MassIVEi | O00161 |
MaxQBi | O00161 |
PaxDbi | O00161 |
PeptideAtlasi | O00161 |
PRIDEi | O00161 |
ProteomicsDBi | 47748 [O00161-1] 47749 [O00161-2] |
2D gel databases
OGPi | O00161 |
PTM databases
iPTMneti | O00161 |
MetOSitei | O00161 |
PhosphoSitePlusi | O00161 |
SwissPalmi | O00161 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000092531, Expressed in chorionic villus and 243 other tissues |
ExpressionAtlasi | O00161, baseline and differential |
Genevisiblei | O00161, HS |
Organism-specific databases
HPAi | ENSG00000092531, Low tissue specificity |
Interactioni
Subunit structurei
Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3 (PubMed:12556468).
Found in a complex with VAMP8 and STX1A (PubMed:12130530).
Found in a complex with VAMP8 and STX4 in pancreas (By similarity).
Interacts simultaneously with SNAPIN and SYN4 (By similarity).
Interacts with STX1A (By similarity).
Interacts with STX12 (By similarity).
Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity).
Interacts with ZDHHC17 (via ANK repeats) (PubMed:28882895).
By similarity3 PublicationsBinary interactionsi
Hide detailsO00161
GO - Molecular functioni
- syntaxin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114303, 89 interactors |
CORUMi | O00161 |
IntActi | O00161, 50 interactors |
MINTi | O00161 |
STRINGi | 9606.ENSP00000249647 |
Miscellaneous databases
RNActi | O00161, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O00161 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O00161 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 76 | t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 146 – 208 | t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 23 – 76 | 1 PublicationAdd BLAST | 54 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG3065, Eukaryota |
GeneTreei | ENSGT00950000182843 |
HOGENOMi | CLU_096939_0_0_1 |
InParanoidi | O00161 |
OMAi | QINRTHV |
OrthoDBi | 1358184at2759 |
PhylomeDBi | O00161 |
TreeFami | TF315125 |
Family and domain databases
InterProi | View protein in InterPro IPR000928, SNAP-25_dom IPR000727, T_SNARE_dom |
Pfami | View protein in Pfam PF00835, SNAP-25, 1 hit |
SMARTi | View protein in SMART SM00397, t_SNARE, 2 hits |
PROSITEi | View protein in PROSITE PS50192, T_SNARE, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDNLSSEEIQ QRAHQITDES LESTRRILGL AIESQDAGIK TITMLDEQKE
60 70 80 90 100
QLNRIEEGLD QINKDMRETE KTLTELNKCC GLCVCPCNRT KNFESGKAYK
110 120 130 140 150
TTWGDGGENS PCNVVSKQPG PVTNGQLQQP TTGAASGGYI KRITNDARED
160 170 180 190 200
EMEENLTQVG SILGNLKDMA LNIGNEIDAQ NPQIKRITDK ADTNRDRIDI
210
ANARAKKLID S
The sequence of this isoform differs from the canonical sequence as follows:
89-89: R → S
90-142: Missing.
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BV99 | H3BV99_HUMAN | Synaptosomal-associated protein | SNAP23 | 142 | Annotation score: | ||
H3BP15 | H3BP15_HUMAN | Synaptosomal-associated protein | SNAP23 | 149 | Annotation score: | ||
H3BQY9 | H3BQY9_HUMAN | Synaptosomal-associated protein | SNAP23 | 154 | Annotation score: | ||
H3BM38 | H3BM38_HUMAN | Synaptosomal-associated protein | SNAP23 | 125 | Annotation score: | ||
H3BR18 | H3BR18_HUMAN | Synaptosomal-associated protein | SNAP23 | 109 | Annotation score: | ||
H3BU94 | H3BU94_HUMAN | Synaptosomal-associated protein 23 | SNAP23 | 53 | Annotation score: | ||
H3BNE1 | H3BNE1_HUMAN | Synaptosomal-associated protein 23 | SNAP23 | 97 | Annotation score: | ||
H3BPJ0 | H3BPJ0_HUMAN | Synaptosomal-associated protein 23 | SNAP23 | 127 | Annotation score: | ||
H3BR99 | H3BR99_HUMAN | Synaptosomal-associated protein 23 | SNAP23 | 90 | Annotation score: | ||
H3BNG6 | H3BNG6_HUMAN | Synaptosomal-associated protein 23 | SNAP23 | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 135 | A → V in AAC50537 (PubMed:8663154).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006187 | 89 | R → S in isoform SNAP-23b. 1 Publication | 1 | |
Alternative sequenceiVSP_006188 | 90 – 142 | Missing in isoform SNAP-23b. 1 PublicationAdd BLAST | 53 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U55936 mRNA Translation: AAC50537.1 Y09567 mRNA Translation: CAA70760.1 Y09568 mRNA Translation: CAA70761.1 AJ011915 mRNA Translation: CAA09864.1 AJ278972, AJ278973, AJ278974 Genomic DNA Translation: CAC07504.1 BT006916 mRNA Translation: AAP35562.1 CR457212 mRNA Translation: CAG33493.1 BC000148 mRNA Translation: AAH00148.1 BC003686 mRNA Translation: AAH03686.1 BC022890 mRNA Translation: AAH22890.1 |
CCDSi | CCDS10087.1 [O00161-1] CCDS10088.1 [O00161-2] |
PIRi | JC5296 JC5297 |
RefSeqi | NP_003816.2, NM_003825.3 [O00161-1] NP_570710.1, NM_130798.2 [O00161-2] |
Genome annotation databases
Ensembli | ENST00000249647; ENSP00000249647; ENSG00000092531 [O00161-1] ENST00000349777; ENSP00000207062; ENSG00000092531 [O00161-2] ENST00000397138; ENSP00000380327; ENSG00000092531 [O00161-2] |
GeneIDi | 8773 |
KEGGi | hsa:8773 |
UCSCi | uc001zpz.3, human [O00161-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U55936 mRNA Translation: AAC50537.1 Y09567 mRNA Translation: CAA70760.1 Y09568 mRNA Translation: CAA70761.1 AJ011915 mRNA Translation: CAA09864.1 AJ278972, AJ278973, AJ278974 Genomic DNA Translation: CAC07504.1 BT006916 mRNA Translation: AAP35562.1 CR457212 mRNA Translation: CAG33493.1 BC000148 mRNA Translation: AAH00148.1 BC003686 mRNA Translation: AAH03686.1 BC022890 mRNA Translation: AAH22890.1 |
CCDSi | CCDS10087.1 [O00161-1] CCDS10088.1 [O00161-2] |
PIRi | JC5296 JC5297 |
RefSeqi | NP_003816.2, NM_003825.3 [O00161-1] NP_570710.1, NM_130798.2 [O00161-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NHL | X-ray | 2.30 | A | 23-76 | [»] | |
3ZUS | X-ray | 2.95 | A/B/C/D | 150-211 | [»] | |
SMRi | O00161 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114303, 89 interactors |
CORUMi | O00161 |
IntActi | O00161, 50 interactors |
MINTi | O00161 |
STRINGi | 9606.ENSP00000249647 |
Protein family/group databases
TCDBi | 1.F.1.1.1, the synaptosomal vesicle fusion pore (svf-pore) family |
PTM databases
iPTMneti | O00161 |
MetOSitei | O00161 |
PhosphoSitePlusi | O00161 |
SwissPalmi | O00161 |
Genetic variation databases
BioMutai | SNAP23 |
2D gel databases
OGPi | O00161 |
Proteomic databases
EPDi | O00161 |
jPOSTi | O00161 |
MassIVEi | O00161 |
MaxQBi | O00161 |
PaxDbi | O00161 |
PeptideAtlasi | O00161 |
PRIDEi | O00161 |
ProteomicsDBi | 47748 [O00161-1] 47749 [O00161-2] |
Protocols and materials databases
Antibodypediai | 712, 412 antibodies |
DNASUi | 8773 |
Genome annotation databases
Ensembli | ENST00000249647; ENSP00000249647; ENSG00000092531 [O00161-1] ENST00000349777; ENSP00000207062; ENSG00000092531 [O00161-2] ENST00000397138; ENSP00000380327; ENSG00000092531 [O00161-2] |
GeneIDi | 8773 |
KEGGi | hsa:8773 |
UCSCi | uc001zpz.3, human [O00161-1] |
Organism-specific databases
CTDi | 8773 |
DisGeNETi | 8773 |
GeneCardsi | SNAP23 |
HGNCi | HGNC:11131, SNAP23 |
HPAi | ENSG00000092531, Low tissue specificity |
MIMi | 602534, gene |
neXtProti | NX_O00161 |
OpenTargetsi | ENSG00000092531 |
PharmGKBi | PA35979 |
VEuPathDBi | HostDB:ENSG00000092531.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3065, Eukaryota |
GeneTreei | ENSGT00950000182843 |
HOGENOMi | CLU_096939_0_0_1 |
InParanoidi | O00161 |
OMAi | QINRTHV |
OrthoDBi | 1358184at2759 |
PhylomeDBi | O00161 |
TreeFami | TF315125 |
Enzyme and pathway databases
PathwayCommonsi | O00161 |
Reactomei | R-HSA-1236974, ER-Phagosome pathway R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-199992, trans-Golgi Network Vesicle Budding R-HSA-6798695, Neutrophil degranulation |
SIGNORi | O00161 |
Miscellaneous databases
BioGRID-ORCSi | 8773, 402 hits in 882 CRISPR screens |
ChiTaRSi | SNAP23, human |
EvolutionaryTracei | O00161 |
GeneWikii | SNAP23 |
GenomeRNAii | 8773 |
Pharosi | O00161, Tbio |
PROi | PR:O00161 |
RNActi | O00161, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000092531, Expressed in chorionic villus and 243 other tissues |
ExpressionAtlasi | O00161, baseline and differential |
Genevisiblei | O00161, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000928, SNAP-25_dom IPR000727, T_SNARE_dom |
Pfami | View protein in Pfam PF00835, SNAP-25, 1 hit |
SMARTi | View protein in SMART SM00397, t_SNARE, 2 hits |
PROSITEi | View protein in PROSITE PS50192, T_SNARE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SNP23_HUMAN | |
Accessioni | O00161Primary (citable) accession number: O00161 Secondary accession number(s): O00162, Q13602, Q6IAE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 208 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families