UniProtKB - O00154 (BACH_HUMAN)
Protein
Cytosolic acyl coenzyme A thioester hydrolase
Gene
ACOT7
Organism
Homo sapiens (Human)
Status
Functioni
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051). Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051).1 Publication1 Publication
Catalytic activityi
- EC:3.1.2.21 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 66 | By similarity | 1 | |
Active sitei | 255 | By similarity | 1 |
GO - Molecular functioni
- acyl-CoA hydrolase activity Source: GO_Central
- carboxylic ester hydrolase activity Source: UniProtKB-KW
- fatty-acyl-CoA binding Source: BHF-UCL
- long-chain fatty acyl-CoA binding Source: BHF-UCL
- myristoyl-CoA hydrolase activity Source: UniProtKB-EC
- palmitoyl-CoA hydrolase activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
GO - Biological processi
- acyl-CoA metabolic process Source: GO_Central
- coenzyme A biosynthetic process Source: BHF-UCL
- fatty acid metabolic process Source: GO_Central
- long-chain fatty-acyl-CoA catabolic process Source: BHF-UCL
- medium-chain fatty acid biosynthetic process Source: BHF-UCL
- medium-chain fatty-acyl-CoA catabolic process Source: BHF-UCL
- palmitic acid biosynthetic process Source: BHF-UCL
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:HS01875-MONOMER |
BRENDAi | 3.1.2.2, 2681 3.1.2.20, 2681 |
PathwayCommonsi | O00154 |
Reactomei | R-HSA-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | O00154 |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic acyl coenzyme A thioester hydrolase (EC:3.1.2.21 Publication)Alternative name(s): Acyl-CoA thioesterase 7 Brain acyl-CoA hydrolase Short name: BACH Short name: hBACH1 Publication CTE-IIa Short name: CTE-II Long chain acyl-CoA thioester hydrolase |
Gene namesi | Name:ACOT7 Synonyms:BACH |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24157, ACOT7 |
MIMi | 602587, gene |
neXtProti | NX_O00154 |
VEuPathDBi | HostDB:ENSG00000097021.19 |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Cytosol
- cytosol 1 Publication
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- Mitochondrion 1 Publication
Cytosol
- cytosol Source: BHF-UCL
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: HPA
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Organism-specific databases
DisGeNETi | 11332 |
OpenTargetsi | ENSG00000097021 |
PharmGKBi | PA142672655 |
Miscellaneous databases
Pharosi | O00154, Tbio |
Genetic variation databases
BioMutai | ACOT7 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053806 | 1 – 380 | Cytosolic acyl coenzyme A thioester hydrolaseAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 168 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 198 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 283 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | CPTAC-303 CPTAC-304 |
EPDi | O00154 |
jPOSTi | O00154 |
MassIVEi | O00154 |
MaxQBi | O00154 |
PaxDbi | O00154 |
PeptideAtlasi | O00154 |
PRIDEi | O00154 |
ProteomicsDBi | 47737 [O00154-1] 47738 [O00154-2] 47739 [O00154-3] 47740 [O00154-4] 47741 [O00154-5] 47742 [O00154-6] |
TopDownProteomicsi | O00154-1 [O00154-1] |
2D gel databases
UCD-2DPAGEi | O00154 |
PTM databases
iPTMneti | O00154 |
MetOSitei | O00154 |
PhosphoSitePlusi | O00154 |
SwissPalmi | O00154 |
Expressioni
Tissue specificityi
Isoform 4 is expressed exclusively in brain.1 Publication
Gene expression databases
Bgeei | ENSG00000097021, Expressed in prefrontal cortex and 217 other tissues |
ExpressionAtlasi | O00154, baseline and differential |
Genevisiblei | O00154, HS |
Organism-specific databases
HPAi | ENSG00000097021, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Homohexamer.
By similarityBinary interactionsi
Hide detailsO00154
Isoform 4 [O00154-4]
GO - Molecular functioni
- protein homodimerization activity Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 116460, 74 interactors |
IntActi | O00154, 49 interactors |
MINTi | O00154 |
STRINGi | 9606.ENSP00000367086 |
Miscellaneous databases
RNActi | O00154, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O00154 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O00154 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 168 | HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 224 – 338 | HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST | 115 |
Domaini
Both HotDog ACOT-type hydrolase domains are required for efficient activity.By similarity
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2763, Eukaryota |
GeneTreei | ENSGT00940000155229 |
HOGENOMi | CLU_050164_0_0_1 |
InParanoidi | O00154 |
OMAi | PTDVNWG |
OrthoDBi | 1069806at2759 |
PhylomeDBi | O00154 |
TreeFami | TF329579 |
Family and domain databases
InterProi | View protein in InterPro IPR040170, Cytosol_ACT IPR033120, HOTDOG_ACOT IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
PANTHERi | PTHR11049, PTHR11049, 2 hits |
Pfami | View protein in Pfam PF03061, 4HBT, 2 hits |
SUPFAMi | SSF54637, SSF54637, 2 hits |
PROSITEi | View protein in PROSITE PS51770, HOTDOG_ACOT, 2 hits |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O00154-1) [UniParc]FASTAAdd to basket
Also known as: B, HBACHb
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKLLARALRL CEFGRQASSR RLVAGQGCVG PRRGCCAPVQ VVGPRADLPP
60 70 80 90 100
CGACITGRIM RPDDANVAGN VHGGTILKMI EEAGAIISTR HCNSQNGERC
110 120 130 140 150
VAALARVERT DFLSPMCIGE VAHVSAEITY TSKHSVEVQV NVMSENILTG
160 170 180 190 200
AKKLTNKATL WYVPLSLKNV DKVLEVPPVV YSRQEQEEEG RKRYEAQKLE
210 220 230 240 250
RMETKWRNGD IVQPVLNPEP NTVSYSQSSL IHLVGPSDCT LHGFVHGGVT
260 270 280 290 300
MKLMDEVAGI VAARHCKTNI VTASVDAINF HDKIRKGCVI TISGRMTFTS
310 320 330 340 350
NKSMEIEVLV DADPVVDSSQ KRYRAASAFF TYVSLSQEGR SLPVPQLVPE
360 370 380
TEDEKKRFEE GKGRYLQMKA KRQGHAEPQP
Isoform 3 (identifier: O00154-3) [UniParc]FASTAAdd to basket
Also known as: A-Xi, hBACHa-Xi
The sequence of this isoform differs from the canonical sequence as follows:
1-57: MKLLARALRLCEFGRQASSRRLVAGQGCVGPRRGCCAPVQVVGPRADLPPCGACITG → MSGPDVETPSAIQIC
287-380: GCVITISGRM...KRQGHAEPQP → AHVMPAGADH...HLGTHDLHEQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketK7EKP8 | K7EKP8_HUMAN | Cytosolic acyl coenzyme A thioester... | ACOT7 | 279 | Annotation score: |
Sequence cautioni
The sequence AAB61211 differs from that shown. Reason: Frameshift.Curated
The sequence AAH17365 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 82 | E → G in BAG51874 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 371 – 372 | KR → DD in AAB61211 (Ref. 3) Curated | 2 | |
Sequence conflicti | 377 – 378 | EP → DA in AAB61211 (Ref. 3) Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000151 | 1 – 58 | MKLLA…CITGR → MLLLRRSLSLNVLRKEVDRA CFGEKAKQ in isoform 5. 2 PublicationsAdd BLAST | 58 | |
Alternative sequenceiVSP_000152 | 1 – 57 | MKLLA…ACITG → MSGPDVETPSAIQIC in isoform 2, isoform 3 and isoform 4. 6 PublicationsAdd BLAST | 57 | |
Alternative sequenceiVSP_000153 | 1 – 57 | MKLLA…ACITG → MAFQLS in isoform 6. 2 PublicationsAdd BLAST | 57 | |
Alternative sequenceiVSP_047094 | 1 – 57 | MKLLA…ACITG → MARPGLIHSAPGLPDTCALL QPPAASAAAAPSMSGPDVET PSAIQIC in isoform 7. 1 PublicationAdd BLAST | 57 | |
Alternative sequenceiVSP_000154 | 287 – 380 | GCVIT…AEPQP → AHVMPAGADHTAPSSSPSTG TKCSLLRHHHLGTHDLHEQ in isoform 3. 1 PublicationAdd BLAST | 94 | |
Alternative sequenceiVSP_000155 | 287 – 288 | GC → AP in isoform 2. 1 Publication | 2 | |
Alternative sequenceiVSP_000156 | 289 – 380 | Missing in isoform 2. 1 PublicationAdd BLAST | 92 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88894 mRNA Translation: BAA24350.1 AB074415 mRNA Translation: BAC20174.1 AB074416 mRNA Translation: BAC20175.1 AB074417 mRNA Translation: BAC20176.1 AB074418 mRNA Translation: BAC20177.1 AB074419 mRNA Translation: BAC20178.1 U91316 mRNA Translation: AAB61211.1 Frameshift. BT006888 mRNA Translation: AAP35534.1 AK289572 mRNA Translation: BAF82261.1 AK290097 mRNA Translation: BAF82786.1 AK291583 mRNA Translation: BAF84272.1 AK292202 mRNA Translation: BAF84891.1 AK057168 mRNA Translation: BAG51874.1 AL031847 Genomic DNA No translation available. AL031848 Genomic DNA No translation available. CH471130 Genomic DNA Translation: EAW71528.1 CH471130 Genomic DNA Translation: EAW71529.1 CH471130 Genomic DNA Translation: EAW71530.1 BC017365 mRNA Translation: AAH17365.2 Different initiation. |
CCDSi | CCDS30573.1 [O00154-7] CCDS65.1 [O00154-1] CCDS66.1 [O00154-5] CCDS67.1 [O00154-6] |
PIRi | JC7161 |
RefSeqi | NP_009205.3, NM_007274.3 [O00154-7] NP_863654.1, NM_181864.2 [O00154-1] NP_863655.1, NM_181865.2 [O00154-5] NP_863656.1, NM_181866.2 [O00154-6] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88894 mRNA Translation: BAA24350.1 AB074415 mRNA Translation: BAC20174.1 AB074416 mRNA Translation: BAC20175.1 AB074417 mRNA Translation: BAC20176.1 AB074418 mRNA Translation: BAC20177.1 AB074419 mRNA Translation: BAC20178.1 U91316 mRNA Translation: AAB61211.1 Frameshift. BT006888 mRNA Translation: AAP35534.1 AK289572 mRNA Translation: BAF82261.1 AK290097 mRNA Translation: BAF82786.1 AK291583 mRNA Translation: BAF84272.1 AK292202 mRNA Translation: BAF84891.1 AK057168 mRNA Translation: BAG51874.1 AL031847 Genomic DNA No translation available. AL031848 Genomic DNA No translation available. CH471130 Genomic DNA Translation: EAW71528.1 CH471130 Genomic DNA Translation: EAW71529.1 CH471130 Genomic DNA Translation: EAW71530.1 BC017365 mRNA Translation: AAH17365.2 Different initiation. |
CCDSi | CCDS30573.1 [O00154-7] CCDS65.1 [O00154-1] CCDS66.1 [O00154-5] CCDS67.1 [O00154-6] |
PIRi | JC7161 |
RefSeqi | NP_009205.3, NM_007274.3 [O00154-7] NP_863654.1, NM_181864.2 [O00154-1] NP_863655.1, NM_181865.2 [O00154-5] NP_863656.1, NM_181866.2 [O00154-6] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QQ2 | X-ray | 2.80 | A/B/C/D/E/F/G/H/I/J/K/L | 209-378 | [»] | |
SMRi | O00154 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116460, 74 interactors |
IntActi | O00154, 49 interactors |
MINTi | O00154 |
STRINGi | 9606.ENSP00000367086 |
PTM databases
iPTMneti | O00154 |
MetOSitei | O00154 |
PhosphoSitePlusi | O00154 |
SwissPalmi | O00154 |
Genetic variation databases
BioMutai | ACOT7 |
2D gel databases
UCD-2DPAGEi | O00154 |
Proteomic databases
CPTACi | CPTAC-303 CPTAC-304 |
EPDi | O00154 |
jPOSTi | O00154 |
MassIVEi | O00154 |
MaxQBi | O00154 |
PaxDbi | O00154 |
PeptideAtlasi | O00154 |
PRIDEi | O00154 |
ProteomicsDBi | 47737 [O00154-1] 47738 [O00154-2] 47739 [O00154-3] 47740 [O00154-4] 47741 [O00154-5] 47742 [O00154-6] |
TopDownProteomicsi | O00154-1 [O00154-1] |
Protocols and materials databases
Antibodypediai | 12967, 155 antibodies |
DNASUi | 11332 |
Genome annotation databases
Organism-specific databases
CTDi | 11332 |
DisGeNETi | 11332 |
GeneCardsi | ACOT7 |
HGNCi | HGNC:24157, ACOT7 |
HPAi | ENSG00000097021, Tissue enhanced (brain) |
MIMi | 602587, gene |
neXtProti | NX_O00154 |
OpenTargetsi | ENSG00000097021 |
PharmGKBi | PA142672655 |
VEuPathDBi | HostDB:ENSG00000097021.19 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2763, Eukaryota |
GeneTreei | ENSGT00940000155229 |
HOGENOMi | CLU_050164_0_0_1 |
InParanoidi | O00154 |
OMAi | PTDVNWG |
OrthoDBi | 1069806at2759 |
PhylomeDBi | O00154 |
TreeFami | TF329579 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BioCyci | MetaCyc:HS01875-MONOMER |
BRENDAi | 3.1.2.2, 2681 3.1.2.20, 2681 |
PathwayCommonsi | O00154 |
Reactomei | R-HSA-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | O00154 |
Miscellaneous databases
BioGRID-ORCSi | 11332, 3 hits in 875 CRISPR screens |
ChiTaRSi | ACOT7, human |
EvolutionaryTracei | O00154 |
GeneWikii | ACOT7 |
GenomeRNAii | 11332 |
Pharosi | O00154, Tbio |
PROi | PR:O00154 |
RNActi | O00154, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000097021, Expressed in prefrontal cortex and 217 other tissues |
ExpressionAtlasi | O00154, baseline and differential |
Genevisiblei | O00154, HS |
Family and domain databases
InterProi | View protein in InterPro IPR040170, Cytosol_ACT IPR033120, HOTDOG_ACOT IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
PANTHERi | PTHR11049, PTHR11049, 2 hits |
Pfami | View protein in Pfam PF03061, 4HBT, 2 hits |
SUPFAMi | SSF54637, SSF54637, 2 hits |
PROSITEi | View protein in PROSITE PS51770, HOTDOG_ACOT, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BACH_HUMAN | |
Accessioni | O00154Primary (citable) accession number: O00154 Secondary accession number(s): A8K0K7 Q9Y540 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 12, 2003 | |
Last modified: | February 10, 2021 | |
This is version 190 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references