SGK1

Functionⁱ

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na⁺ retention, renal K⁺ elimination, salt appetite, gastric acid secretion, intestinal Na⁺/H⁺ exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na⁺ channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K⁺ channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca²⁺ channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na⁺/dicarboxylate cotransporter: SLC13A2/NADC1, Na⁺-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na⁺/K⁺ ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na⁺ transport than isoform 1.32 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.

Activity regulationⁱ

Two specific sites, one in the kinase domain (Thr-256) and the other in the C-terminal regulatory region (Ser-422), need to be phosphorylated for its full activation. Phosphorylation at Ser-397 and Ser-401 are also essential for its activity. Activated by WNK1, WNK2, WNK3 and WNK4.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	127	ATP		1
Active siteⁱ	222	Proton acceptor		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	104 – 112	ATP		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
calcium channel regulator activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112
chloride channel regulator activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112
potassium channel regulator activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112
protein serine/threonine/tyrosine kinase activity
Source: MGI
Inferred from direct assayⁱ
- 22797923
protein serine/threonine kinase activity Source: GO_Central
sodium channel regulator activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process Source: UniProtKB-KW
cellular response to DNA damage stimulus Source: Ensembl
intracellular signal transduction Source: GO_Central
ion transmembrane transport Source: Reactome
long-term memory
Source: UniProtKB
Traceable author statementⁱ
- 20530112
neuron projection morphogenesis Source: GO_Central
peptidyl-serine phosphorylation Source: GO_Central
positive regulation of transporter activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112
protein phosphorylation
Source: ProtInc
Traceable author statementⁱ
- 9114008
regulation of apoptotic process
Source: UniProtKB
Traceable author statementⁱ
- 20530112
regulation of blood pressure
Source: UniProtKB
Traceable author statementⁱ
- 16460280
regulation of catalytic activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112
regulation of cell growth
Source: UniProtKB
Traceable author statementⁱ
- 20919962
regulation of cell migration
Source: UniProtKB
Traceable author statementⁱ
- 20919962
regulation of cell proliferation
Source: UniProtKB
Traceable author statementⁱ
- 20530112
regulation of DNA-binding transcription factor activity
Source: UniProtKB
Traceable author statementⁱ
- 20530112
regulation of gastric acid secretion
Source: UniProtKB
Traceable author statementⁱ
- 20530112
renal sodium ion absorption
Source: UniProtKB
Traceable author statementⁱ
- 16460280
sodium ion transport
Source: ProtInc
Traceable author statementⁱ
- 10884438

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Apoptosis, Stress response
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.11.1 2681
Reactomeⁱ	R-HSA-2672351 Stimuli-sensing channels R-HSA-6804757 Regulation of TP53 Degradation
SignaLinkⁱ	O00141
SIGNORⁱ	O00141

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine/threonine-protein kinase Sgk1 (EC:2.7.11.1) Alternative name(s): Serum/glucocorticoid-regulated kinase 1
Gene namesⁱ	Name:SGK1 Synonyms:SGK
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 6

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000118515.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:10810 SGK1
MIMⁱ	602958 gene
neXtProtⁱ	NX_O00141

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	127	K → M: Abolishes enzymatic activity. 3 Publications	1
Mutagenesisⁱ	256	T → A: Low activity. 1 Publication	1
Mutagenesisⁱ	256	T → D: Low activity. 1 Publication	1
Mutagenesisⁱ	256	T → E: Low activity. 1 Publication	1
Mutagenesisⁱ	298	Y → A: Abolishes interaction with NEDD4 and NEDD4L. 1 Publication	1
Mutagenesisⁱ	422	S → A: Low activity. 4 Publications	1
Mutagenesisⁱ	422	S → D: 10-fold activation. 4 Publications	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	6446
Open Targets More...OpenTargetsⁱ	ENSG00000118515
PharmGKBⁱ	PA162403013

Chemistry databases

ChEMBLⁱ	CHEMBL2343
Drug and drug target database More...DrugBankⁱ	DB03247 Riboflavin Monophosphate
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	1534

Polymorphism and mutation databases

BioMutaⁱ	SGK1

BioMutaⁱ

SGK1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086642	1 – 431	Serine/threonine-protein kinase Sgk1Add BLAST		431

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	74	PhosphoserineCombined sources	1
Modified residueⁱ	78	Phosphoserine; by MAPK71 Publication	1
Disulfide bondⁱ	193	Interchain (with C-258)1 Publication
Modified residueⁱ	256	Phosphothreonine; by PDPK11 Publication	1
Disulfide bondⁱ	258	Interchain (with C-193)1 Publication
Modified residueⁱ	369	Phosphothreonine; by PKA1 Publication	1
Modified residueⁱ	397	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	401	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	422	Phosphoserine3 Publications	1

Post-translational modificationⁱ

Regulated by phosphorylation. Activated by phosphorylation on Ser-422 by mTORC2, transforming it into a substrate for PDPK1 which phosphorylates it on Thr-256. Phosphorylation on Ser-397 and Ser-401 are also essential for its activity. Phosphorylation on Ser-78 by MAPK7 is required for growth factor-induced cell cycle progression.6 Publications

Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability.1 Publication

Keywords - PTMⁱ

Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	O00141
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O00141
PaxDbⁱ	O00141
PeptideAtlas More...PeptideAtlasⁱ	O00141
PRIDEⁱ	O00141
ProteomicsDBⁱ	47726 47727 [O00141-2] 47728 [O00141-3] 47729 [O00141-4] 47730 [O00141-5]

PTM databases

iPTMnetⁱ	O00141
PhosphoSitePlusⁱ	O00141

Expressionⁱ

Tissue specificityⁱ

Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.2 Publications

Inductionⁱ

Induced by a very large spectrum of stimuli distinct from glucocorticoids and serum. These include aldosterone, cell shrinkage, cell swelling, TGF-beta, ischemic injury of the brain, neuronal excitotoxicity memory consolidation, chronic viral hepatitis, DNA-damaging agents, vitamin D3 psychophysiological stress, iron, glucose, EDN1, CSF2, fibroblast growth factor, platelet-derived growth factor, phorbolesters, follicle-stimulating hormone, sorbitol, heat shock, oxidative stress, UV irradiation, and p53/TP53. Many of these stimuli are highly cell-specific, as is the case, for example for aldosterone, which has been found to stimulate its expression only in cells derived from aldosterone-responsive epithelia. Isoform 2 is not induced by glucocorticoids but by excessive extracellular glucose and by TGFB1, in cultured cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000118515 Expressed in 233 organ(s), highest expression level in female gonad
CleanExⁱ	HS_SGK1
ExpressionAtlasⁱ	O00141 baseline and differential
Genevisibleⁱ	O00141 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB022085 CAB025148 HPA051251

HPAⁱ

CAB022085
CAB025148
HPA051251

Interactionⁱ

Subunit structureⁱ

Homodimer; disulfide-linked. Forms a trimeric complex with FBXW7 and NOTCH1. Interacts with MAPK3/ERK1, MAPK1/ERK2, MAP2K1/MEK1, MAP2K2/MEK2, NEDD4, NEDD4L, MAPT/TAU, MAPK7, CREB1, SLC9A3R2/NHERF2 and KCNJ1/ROMK1. Associates with the mammalian target of rapamycin complex 2 (mTORC2) via an interaction with MAPKAP1/SIN1.8 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
TSC2	P49815	4	EBI-1042854,EBI-396587
Wnk4	Q80UE6	2	EBI-1042854,EBI-295378	From Mus musculus.

Protein-protein interaction databases

BioGridⁱ	112344, 63 interactors
CORUMⁱ	O00141
Database of interacting proteins More...DIPⁱ	DIP-42464N
ELMⁱ	O00141
IntActⁱ	O00141, 16 interactors
Molecular INTeraction database More...MINTⁱ	O00141
STRINGⁱ	9606.ENSP00000356832

Chemistry databases

BindingDBⁱ	O00141

BindingDBⁱ

O00141

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	95 – 97	Combined sources	3
Beta strandⁱ	98 – 105	Combined sources	8
Beta strandⁱ	111 – 117	Combined sources	7
Turnⁱ	118 – 120	Combined sources	3
Beta strandⁱ	123 – 130	Combined sources	8
Helixⁱ	131 – 133	Combined sources	3
Beta strandⁱ	162 – 167	Combined sources	6
Beta strandⁱ	169 – 177	Combined sources	9
Helixⁱ	184 – 191	Combined sources	8
Helixⁱ	196 – 215	Combined sources	20
Helixⁱ	225 – 227	Combined sources	3
Beta strandⁱ	228 – 230	Combined sources	3
Beta strandⁱ	232 – 234	Combined sources	3
Beta strandⁱ	236 – 238	Combined sources	3
Helixⁱ	245 – 247	Combined sources	3
Beta strandⁱ	256 – 258	Combined sources	3
Helixⁱ	266 – 269	Combined sources	4
Helixⁱ	277 – 292	Combined sources	16
Helixⁱ	302 – 311	Combined sources	10
Beta strandⁱ	318 – 320	Combined sources	3
Helixⁱ	322 – 331	Combined sources	10
Helixⁱ	336 – 338	Combined sources	3
Turnⁱ	340 – 345	Combined sources	6
Helixⁱ	346 – 350	Combined sources	5
Helixⁱ	353 – 355	Combined sources	3
Helixⁱ	360 – 364	Combined sources	5

Show more details

3D structure databases

ProteinModelPortalⁱ	O00141
SMRⁱ	O00141
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	O00141

EvolutionaryTraceⁱ

O00141

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	98 – 355	Protein kinasePROSITE-ProRule annotationAdd BLAST		258
Domainⁱ	356 – 431	AGC-kinase C-terminalAdd BLAST		76

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 60	Necessary for localization to the mitochondriaAdd BLAST		60

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	131 – 141	Nuclear localization signal1 PublicationAdd BLAST		11

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	131 – 141	Glu/Lys-richAdd BLAST		11

Domainⁱ

Isoform 2 subcellular localization at the cell membrane and resistance to proteasomal degradation is mediated by the sequences within the first 120 amino acids.

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0598 Eukaryota ENOG410XNPH LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00920000148938
HOVERGENⁱ	HBG108317
InParanoidⁱ	O00141
KEGG Orthology (KO) More...KOⁱ	K13302
OMAⁱ	EVKEPCN
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06FF
PhylomeDBⁱ	O00141
TreeFamⁱ	TF320906

Family and domain databases

InterProⁱ	View protein in InterPro IPR000961 AGC-kinase_C IPR011009 Kinase-like_dom_sf IPR017892 Pkinase_C IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit PF00433 Pkinase_C, 1 hit
SMARTⁱ	View protein in SMART SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (5+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 5 isoformsⁱ produced by alternative promoter usage and alternative splicing. Align Add to basket

This entry has 5 described isoforms and 5 potential isoforms that are computationally mapped.ⁱShow all

Isoform 1 (identifier: O00141-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVKTEAAKG TLTYSRMRGM VAILIAFMKQ RRMGLNDFIQ KIANNSYACK 
        60         70         80         90        100
HPEVQSILKI SQPQEPELMN ANPSPPPSPS QQINLGPSSN PHAKPSDFHF 
       110        120        130        140        150
LKVIGKGSFG KVLLARHKAE EVFYAVKVLQ KKAILKKKEE KHIMSERNVL 
       160        170        180        190        200
LKNVKHPFLV GLHFSFQTAD KLYFVLDYIN GGELFYHLQR ERCFLEPRAR 
       210        220        230        240        250
FYAAEIASAL GYLHSLNIVY RDLKPENILL DSQGHIVLTD FGLCKENIEH 
       260        270        280        290        300
NSTTSTFCGT PEYLAPEVLH KQPYDRTVDW WCLGAVLYEM LYGLPPFYSR 
       310        320        330        340        350
NTAEMYDNIL NKPLQLKPNI TNSARHLLEG LLQKDRTKRL GAKDDFMEIK 
       360        370        380        390        400
SHVFFSLINW DDLINKKITP PFNPNVSGPN DLRHFDPEFT EEPVPNSIGK 
       410        420        430 
SPDSVLVTAS VKEAAEAFLG FSYAPPTDSF L

Length:431

Mass (Da):48,942

Last modified:March 7, 2006 - v2

Checksum:ⁱF3697C63AB1F499D

GO

Isoform 2 (identifier: O00141-2) [UniParc]FASTA Add to basket

Also known as: Sgk1.1, Sgk1_v2

The sequence of this isoform differs from the canonical sequence as follows:
1-25: MTVKTEAAKGTLTYSRMRGMVAILI → MVNKDMNGFP...PRTFWTNDDP

« Hide

        10         20         30         40         50
MVNKDMNGFP VKKCSAFQFF KKRVRRWIKS PMVSVDKHQS PSLKYTGSSM 
        60         70         80         90        100
VHIPPGEPDF ESSLCQTCLG EHAFQRGVLP QENESCSWET QSGCEVREPC 
       110        120        130        140        150
NHANILTKPD PRTFWTNDDP AFMKQRRMGL NDFIQKIANN SYACKHPEVQ 
       160        170        180        190        200
SILKISQPQE PELMNANPSP PPSPSQQINL GPSSNPHAKP SDFHFLKVIG 
       210        220        230        240        250
KGSFGKVLLA RHKAEEVFYA VKVLQKKAIL KKKEEKHIMS ERNVLLKNVK 
       260        270        280        290        300
HPFLVGLHFS FQTADKLYFV LDYINGGELF YHLQRERCFL EPRARFYAAE 
       310        320        330        340        350
IASALGYLHS LNIVYRDLKP ENILLDSQGH IVLTDFGLCK ENIEHNSTTS 
       360        370        380        390        400
TFCGTPEYLA PEVLHKQPYD RTVDWWCLGA VLYEMLYGLP PFYSRNTAEM 
       410        420        430        440        450
YDNILNKPLQ LKPNITNSAR HLLEGLLQKD RTKRLGAKDD FMEIKSHVFF 
       460        470        480        490        500
SLINWDDLIN KKITPPFNPN VSGPNDLRHF DPEFTEEPVP NSIGKSPDSV 
       510        520 
LVTASVKEAA EAFLGFSYAP PTDSFL

Note: Produced by alternative promoter usage.

Show »

Length:526

Mass (Da):59,904

Checksum:ⁱ8CE1E9DFB949D5A5

GO

Isoform 3 (identifier: O00141-3) [UniParc]FASTA Add to basket

Also known as: Sgk1.2

The sequence of this isoform differs from the canonical sequence as follows:
1-25: MTVKTEAAKGTLTYSRMRGMVAILI → MGEMQGALARARLESLLRPRHKKRAEAQKRSESFLLSGL

« Hide

        10         20         30         40         50
MGEMQGALAR ARLESLLRPR HKKRAEAQKR SESFLLSGLA FMKQRRMGLN 
        60         70         80         90        100
DFIQKIANNS YACKHPEVQS ILKISQPQEP ELMNANPSPP PSPSQQINLG 
       110        120        130        140        150
PSSNPHAKPS DFHFLKVIGK GSFGKVLLAR HKAEEVFYAV KVLQKKAILK 
       160        170        180        190        200
KKEEKHIMSE RNVLLKNVKH PFLVGLHFSF QTADKLYFVL DYINGGELFY 
       210        220        230        240        250
HLQRERCFLE PRARFYAAEI ASALGYLHSL NIVYRDLKPE NILLDSQGHI 
       260        270        280        290        300
VLTDFGLCKE NIEHNSTTST FCGTPEYLAP EVLHKQPYDR TVDWWCLGAV 
       310        320        330        340        350
LYEMLYGLPP FYSRNTAEMY DNILNKPLQL KPNITNSARH LLEGLLQKDR 
       360        370        380        390        400
TKRLGAKDDF MEIKSHVFFS LINWDDLINK KITPPFNPNV SGPNDLRHFD 
       410        420        430        440 
PEFTEEPVPN SIGKSPDSVL VTASVKEAAE AFLGFSYAPP TDSFL

Note: Produced by alternative promoter usage.

Show »

Length:445

Mass (Da):50,623

Checksum:ⁱDC7076E1F43BCBAB

GO

Isoform 4 (identifier: O00141-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-25: MTVKTEAAKGTLTYSRMRGMVAILI → MKPSKRFFISPPSST

« Hide

        10         20         30         40         50
MKPSKRFFIS PPSSTAFMKQ RRMGLNDFIQ KIANNSYACK HPEVQSILKI 
        60         70         80         90        100
SQPQEPELMN ANPSPPPSPS QQINLGPSSN PHAKPSDFHF LKVIGKGSFG 
       110        120        130        140        150
KVLLARHKAE EVFYAVKVLQ KKAILKKKEE KHIMSERNVL LKNVKHPFLV 
       160        170        180        190        200
GLHFSFQTAD KLYFVLDYIN GGELFYHLQR ERCFLEPRAR FYAAEIASAL 
       210        220        230        240        250
GYLHSLNIVY RDLKPENILL DSQGHIVLTD FGLCKENIEH NSTTSTFCGT 
       260        270        280        290        300
PEYLAPEVLH KQPYDRTVDW WCLGAVLYEM LYGLPPFYSR NTAEMYDNIL 
       310        320        330        340        350
NKPLQLKPNI TNSARHLLEG LLQKDRTKRL GAKDDFMEIK SHVFFSLINW 
       360        370        380        390        400
DDLINKKITP PFNPNVSGPN DLRHFDPEFT EEPVPNSIGK SPDSVLVTAS 
       410        420 
VKEAAEAFLG FSYAPPTDSF L

Note: Produced by alternative splicing of isoform 1.

Show »

Length:421

Mass (Da):47,910

Checksum:ⁱ6BDCD1FA7D9AFD0E

GO

Isoform 5 (identifier: O00141-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-25: MTVKTEAAKGTLTYSRMRGMVAILI → MSSQSSSLSE...KEEAIKPPLK

« Hide

        10         20         30         40         50
MSSQSSSLSE ACSREAYSSH NWALPPASRS NPQPAYPWAT RRMKEEAIKP 
        60         70         80         90        100
PLKAFMKQRR MGLNDFIQKI ANNSYACKHP EVQSILKISQ PQEPELMNAN 
       110        120        130        140        150
PSPPPSPSQQ INLGPSSNPH AKPSDFHFLK VIGKGSFGKV LLARHKAEEV 
       160        170        180        190        200
FYAVKVLQKK AILKKKEEKH IMSERNVLLK NVKHPFLVGL HFSFQTADKL 
       210        220        230        240        250
YFVLDYINGG ELFYHLQRER CFLEPRARFY AAEIASALGY LHSLNIVYRD 
       260        270        280        290        300
LKPENILLDS QGHIVLTDFG LCKENIEHNS TTSTFCGTPE YLAPEVLHKQ 
       310        320        330        340        350
PYDRTVDWWC LGAVLYEMLY GLPPFYSRNT AEMYDNILNK PLQLKPNITN 
       360        370        380        390        400
SARHLLEGLL QKDRTKRLGA KDDFMEIKSH VFFSLINWDD LINKKITPPF 
       410        420        430        440        450
NPNVSGPNDL RHFDPEFTEE PVPNSIGKSP DSVLVTASVK EAAEAFLGFS 

YAPPTDSFL

Note: Produced by alternative promoter usage.

Show »

Length:459

Mass (Da):52,119

Checksum:ⁱ1B108E7CF17935E5

GO

Computationally mapped potential isoform sequencesⁱ

There are 5 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation
E9PR89	E9PR89_HUMAN	Non-specific serine/threonine prote... Non-specific serine/threonine protein kinase, EC 2.7.11.1	SGK1	387	Annotation score:
E9PJN2	E9PJN2_HUMAN	Serine/threonine-protein kinase Sgk... Serine/threonine-protein kinase Sgk1	SGK1	144	Annotation score:
Q7Z3I4	Q7Z3I4_HUMAN	Serine/threonine-protein kinase Sgk... Serine/threonine-protein kinase Sgk1	SGK1 DKFZp686H1615	153	Annotation score:
E9PP33	E9PP33_HUMAN	Serine/threonine-protein kinase Sgk... Serine/threonine-protein kinase Sgk1	SGK1	60	Annotation score:
H0YCJ3	H0YCJ3_HUMAN	Serine/threonine-protein kinase Sgk... Serine/threonine-protein kinase Sgk1	SGK1	55	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	62	Q → E in CAR58097 (Ref. 4) Curated	1
Sequence conflictⁱ	152	K → R in CAR58096 (Ref. 4) Curated	1
Sequence conflictⁱ	196	E → G in CAR58095 (Ref. 4) Curated	1
Sequence conflictⁱ	228	I → V in BAH12848 (PubMed:14702039).Curated	1
Sequence conflictⁱ	371	P → R in CAR58097 (Ref. 4) Curated	1
Sequence conflictⁱ	381	D → E in CAA71138 (PubMed:9114008).Curated	1
Sequence conflictⁱ	381	D → E in CAA04146 (PubMed:9722955).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_041071	219	V → I1 PublicationCorresponds to variant dbSNP:rs34133418Ensembl.		1
Natural variantⁱVAR_041072	342	A → V1 PublicationCorresponds to variant dbSNP:rs55932330Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_037784	1 – 25	MTVKT…VAILI → MVNKDMNGFPVKKCSAFQFF KKRVRRWIKSPMVSVDKHQS PSLKYTGSSMVHIPPGEPDF ESSLCQTCLGEHAFQRGVLP QENESCSWETQSGCEVREPC NHANILTKPDPRTFWTNDDP in isoform 2. 3 PublicationsAdd BLAST	25
Alternative sequenceⁱVSP_037785	1 – 25	MTVKT…VAILI → MGEMQGALARARLESLLRPR HKKRAEAQKRSESFLLSGL in isoform 3. 2 PublicationsAdd BLAST	25
Alternative sequenceⁱVSP_037786	1 – 25	MTVKT…VAILI → MKPSKRFFISPPSST in isoform 4. 1 PublicationAdd BLAST	25
Alternative sequenceⁱVSP_037787	1 – 25	MTVKT…VAILI → MSSQSSSLSEACSREAYSSH NWALPPASRSNPQPAYPWAT RRMKEEAIKPPLK in isoform 5. 1 PublicationAdd BLAST	25

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y10032 mRNA Translation: CAA71138.1 AJ000512 Genomic DNA Translation: CAA04146.1 EU518415 mRNA Translation: ACD35864.1 FM205707 mRNA Translation: CAR58095.1 FM205708 mRNA Translation: CAR58096.1 FM205709 mRNA Translation: CAR58097.1 FM205710 mRNA Translation: CAR58098.1 AF153609 mRNA Translation: AAD41091.1 AK055077 mRNA Translation: BAG51463.1 AK298688 mRNA Translation: BAH12848.1 AL355881 Genomic DNA No translation available. AL135839 Genomic DNA No translation available. Z84486 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW47991.1 CH471051 Genomic DNA Translation: EAW47992.1 CH471051 Genomic DNA Translation: EAW47993.1 BC001263 mRNA Translation: AAH01263.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS47476.1 [O00141-2] CCDS47477.1 [O00141-5] CCDS47478.1 [O00141-3] CCDS5170.1 [O00141-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001137148.1, NM_001143676.1 [O00141-2] NP_001137149.1, NM_001143677.1 [O00141-5] NP_001137150.1, NM_001143678.1 [O00141-3] NP_001278924.1, NM_001291995.1 NP_005618.2, NM_005627.3 [O00141-1] XP_011534373.1, XM_011536071.1 [O00141-2]
UniGeneⁱ	Hs.510078

Genome annotation databases

Ensemblⁱ	ENST00000237305; ENSP00000237305; ENSG00000118515 [O00141-1] ENST00000367857; ENSP00000356831; ENSG00000118515 [O00141-4] ENST00000367858; ENSP00000356832; ENSG00000118515 [O00141-2] ENST00000413996; ENSP00000396242; ENSG00000118515 [O00141-3] ENST00000528577; ENSP00000434450; ENSG00000118515 [O00141-5]
GeneIDⁱ	6446
KEGGⁱ	hsa:6446
UCSC genome browser More...UCSCⁱ	uc003qen.5 human [O00141-1]

Keywords - Coding sequence diversityⁱ

Alternative promoter usage, Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O00141	Non-specific serine/threonine protein kinase		PONAB		431	UniRef100_O00141
Non-specific serine/threonine protein kinase		PANPA		431
Non-specific serine/threonine protein kinase		NOMLE		431
Non-specific serine/threonine protein kinase		GORGO		431
O00141-3	Non-specific serine/threonine protein kinase		PANTR		445	UniRef100_K7CFP7
Non-specific serine/threonine protein kinase		PONAB		445
Non-specific serine/threonine protein kinase		GORGO		445
O00141-4	Non-specific serine/threonine protein kinase		PANTR		526	UniRef100_H2R6X6
Non-specific serine/threonine protein kinase		PANPA		526
Non-specific serine/threonine protein kinase		PANTR		421
O00141-5	Non-specific serine/threonine protein kinase		PANTR		459	UniRef100_K7BJD5
Non-specific serine/threonine protein kinase		PANPA		459
Non-specific serine/threonine protein kinase		GORGO		459

Protein	Similar proteins		Species	Score	Length	Source
O00141	Serine/threonine-protein kinase Sgk1		MACFA		431	UniRef90_O00141
Non-specific serine/threonine protein kinase		PONAB		431
Non-specific serine/threonine protein kinase		PANPA		431
Non-specific serine/threonine protein kinase		NOMLE		431
Non-specific serine/threonine protein kinase		GORGO		431
+143

Protein	Similar proteins		Species	Score	Length	Source
O00141	Serine/threonine-protein kinase Sgk1		MACFA		431	UniRef50_O00141
Serine/threonine-protein kinase Sgk1		CHICK		432
Serine/threonine-protein kinase Sgk1		MOUSE		431
Serine/threonine-protein kinase Sgk1		BOVIN		431
Serine/threonine-protein kinase Sgk1		RABIT		431
+462

Entry informationⁱ

Entry nameⁱ	SGK1_HUMAN
Accessionⁱ	O00141Primary (citable) accession number: O00141 Secondary accession number(s): B7UUP7 Q9UN56
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	March 7, 2006
	Last modified:	September 12, 2018
	This is version 188 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniProtKB - O00141 (SGK1_HUMAN)

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Serine/threonine-protein kinase Sgk1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

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Keywords - PTMi

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Keywords - Coding sequence diversityi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Computationally mapped potential isoform sequencesⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ