We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - O00092 (PHYA_ASPFU)
Protein
3-phytase A
Gene
phyA
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Functioni
Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
Catalytic activityi
- EC:3.1.3.8
pH dependencei
Highly active from pH 3 to 5 with 4-nitrophenyl phosphate as substrate, and from 2.5 to 7.5 with phytic acid.
Temperature dependencei
Able to withstand temperatures up to 100 degrees Celsius over a period of 20 min, with a loss of only 10% of the initial enzymatic activity.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 81 | NucleophileBy similarity | 1 | |
Active sitei | 360 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- 3-phytase activity Source: UniProtKB-EC
- acid phosphatase activity Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BRENDAi | 3.1.3.26, 508 3.1.3.8, 508 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-phytase A (EC:3.1.3.8)Alternative name(s): 3 phytase A Myo-inositol hexakisphosphate phosphohydrolase A Myo-inositol-hexaphosphate 3-phosphohydrolase A |
Gene namesi | Name:phyA Synonyms:phyA3 ORF Names:AFUA_4G08630 |
Organismi | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
Taxonomic identifieri | 330879 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | 1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000023969 | 27 – 465 | 3-phytase AAdd BLAST | 439 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 39 | 1 Publication | ||
Disulfide bondi | 70 ↔ 412 | 1 Publication | ||
Glycosylationi | 104 | N-linked (GlcNAc...) asparagine | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 205 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 213 ↔ 463 | 1 Publication | ||
Glycosylationi | 228 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 262 ↔ 280 | 1 Publication | ||
Glycosylationi | 337 | N-linked (GlcNAc...) asparagine | 1 | |
Glycosylationi | 350 | N-linked (GlcNAc...) asparagine | 1 | |
Glycosylationi | 374 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 434 ↔ 442 | 1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O00092 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O00092 |
Family & Domainsi
Sequence similaritiesi
Belongs to the histidine acid phosphatase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1382, Eukaryota |
HOGENOMi | CLU_020880_0_0_1 |
InParanoidi | O00092 |
OrthoDBi | 1046588at2759 |
Family and domain databases
CDDi | cd07061, HP_HAP_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR033379, Acid_Pase_AS IPR000560, His_Pase_clade-2 IPR029033, His_PPase_superfam IPR016274, Histidine_acid_Pase_euk |
Pfami | View protein in Pfam PF00328, His_Phos_2, 1 hit |
PIRSFi | PIRSF000894, Acid_phosphatase, 1 hit |
SUPFAMi | SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00616, HIS_ACID_PHOSPHAT_1, 1 hit PS00778, HIS_ACID_PHOSPHAT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O00092-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVTLTFLLSA AYLLSGRVSA APSSAGSKSC DTVDLGYQCS PATSHLWGQY
60 70 80 90 100
SPFFSLEDEL SVSSKLPKDC RITLVQVLSR HGARYPTSSK SKKYKKLVTA
110 120 130 140 150
IQANATDFKG KFAFLKTYNY TLGADDLTPF GEQQLVNSGI KFYQRYKALA
160 170 180 190 200
RSVVPFIRAS GSDRVIASGE KFIEGFQQAK LADPGATNRA APAISVIIPE
210 220 230 240 250
SETFNNTLDH GVCTKFEASQ LGDEVAANFT ALFAPDIRAR AEKHLPGVTL
260 270 280 290 300
TDEDVVSLMD MCSFDTVART SDASQLSPFC QLFTHNEWKK YNYLQSLGKY
310 320 330 340 350
YGYGAGNPLG PAQGIGFTNE LIARLTRSPV QDHTSTNSTL VSNPATFPLN
360 370 380 390 400
ATMYVDFSHD NSMVSIFFAL GLYNGTEPLS RTSVESAKEL DGYSASWVVP
410 420 430 440 450
FGARAYFETM QCKSEKEPLV RALINDRVVP LHGCDVDKLG RCKLNDFVKG
460
LSWARSGGNW GECFS
Sequence cautioni
The sequence EAL89926 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U59804 Genomic DNA Translation: AAB96872.1 AAHF01000005 Genomic DNA Translation: EAL89926.2 Different initiation. AJ419776 Genomic DNA Translation: CAD12029.1 |
RefSeqi | XP_751964.2, XM_746871.2 |
Genome annotation databases
GeneIDi | 3509324 |
KEGGi | afm:AFUA_4G08630 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U59804 Genomic DNA Translation: AAB96872.1 AAHF01000005 Genomic DNA Translation: EAL89926.2 Different initiation. AJ419776 Genomic DNA Translation: CAD12029.1 |
RefSeqi | XP_751964.2, XM_746871.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QWO | X-ray | 1.50 | A | 24-465 | [»] | |
1SK8 | X-ray | 1.65 | A | 27-464 | [»] | |
1SK9 | X-ray | 1.64 | A | 27-464 | [»] | |
1SKA | X-ray | 1.69 | A | 27-464 | [»] | |
1SKB | X-ray | 1.58 | A | 27-464 | [»] | |
SMRi | O00092 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 746128.CADAFUBP00006397 |
Genome annotation databases
GeneIDi | 3509324 |
KEGGi | afm:AFUA_4G08630 |
Phylogenomic databases
eggNOGi | KOG1382, Eukaryota |
HOGENOMi | CLU_020880_0_0_1 |
InParanoidi | O00092 |
OrthoDBi | 1046588at2759 |
Enzyme and pathway databases
BRENDAi | 3.1.3.26, 508 3.1.3.8, 508 |
Miscellaneous databases
EvolutionaryTracei | O00092 |
Family and domain databases
CDDi | cd07061, HP_HAP_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR033379, Acid_Pase_AS IPR000560, His_Pase_clade-2 IPR029033, His_PPase_superfam IPR016274, Histidine_acid_Pase_euk |
Pfami | View protein in Pfam PF00328, His_Phos_2, 1 hit |
PIRSFi | PIRSF000894, Acid_phosphatase, 1 hit |
SUPFAMi | SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00616, HIS_ACID_PHOSPHAT_1, 1 hit PS00778, HIS_ACID_PHOSPHAT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHYA_ASPFU | |
Accessioni | O00092Primary (citable) accession number: O00092 Secondary accession number(s): Q4WPA8, Q8WZJ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families