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Protein

Endonuclease III

Gene

nth

Organism
Methanocaldococcus villosus KIN24-T80
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi180Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi186Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi189Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi197Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, EndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
ORF Names:J422_00486Imported
OrganismiMethanocaldococcus villosus KIN24-T80Imported
Taxonomic identifieri1069083 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000053695 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 178ENDO3cInterPro annotationAdd BLAST148
Domaini243 – 336GIY-YIGInterPro annotationAdd BLAST94

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili44 – 64Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

OrthoDBiPOG093Z0AWD

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
cd10441 GIY-YIG_COG1833, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR002837 DUF123
IPR004036 Endonuclease-III-like_CS2
IPR000305 GIY-YIG_endonuc
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF01986 DUF123, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00465 GIYc, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

N6V3B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMIERLLKR IDKKPAVVKD EPFKVLVAAL ISARTNDNIT EKVMKKLFKE
60 70 80 90 100
VKDLDDLLEI DEERLSSLLY PVGFYRNKAK LLKKLAKVLK ERYNGKIPDN
110 120 130 140 150
LEDLLSLPGV GRKTANLVLT LAFDKDGIAV DTHVHRITNR WEIVDTETPE
160 170 180 190 200
ETEMVLRKKL DRKYWKVINH ILVVFGREIC GVKPKCSICY KEIKELCPYY
210 220 230 240 250
KKLLHFEHII DKFKYKKVSK SKIPNEKGSY ILKIKLKKGR LIRFGKNEKY
260 270 280 290 300
FKSGYYFYVG SAMNGLKNRI ERHLKNEKKM FWHIDYLLNY GKIEEIYITN
310 320 330
NNVECETANE FIKAFDYIEG FGCSDCSCKS HLFYMKL
Length:337
Mass (Da):39,676
Last modified:June 26, 2013 - v1
Checksum:i723B50DCCF748C64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
APMM01000003 Genomic DNA Translation: ENN96748.1
RefSeqiWP_004589779.1, NZ_AQUK01000001.1

Genome annotation databases

EnsemblBacteriaiENN96748; ENN96748; J422_00486
PATRICifig|1069083.5.peg.93

Similar proteinsi

Entry informationi

Entry nameiN6V3B2_9EURY
AccessioniPrimary (citable) accession number: N6V3B2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 26, 2013
Last sequence update: June 26, 2013
Last modified: May 23, 2018
This is version 30 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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