UniProtKB - M9PCQ1 (M9PCQ1_DROME)
Protein
NADPH--cytochrome P450 reductase
Gene
Cpr
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation
Caution
Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
Catalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 212 | FMNUniRule annotation | 1 | |
Binding sitei | 302 | NADPUniRule annotation | 1 | |
Binding sitei | 482 | FADUniRule annotation | 1 | |
Binding sitei | 537 | NADPUniRule annotation | 1 | |
Binding sitei | 640 | NADPUniRule annotation | 1 | |
Binding sitei | 678 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 90 – 95 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 142 – 145 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 177 – 186 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 458 – 461 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 476 – 478 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 492 – 495 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 597 – 598 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 603 – 607 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: UniProtKB-UniRule
- FMN binding Source: UniProtKB-UniRule
- NADP binding Source: UniProtKB-UniRule
- NADPH-hemoprotein reductase activity Source: UniProtKB-UniRule
Keywordsi
Molecular function | OxidoreductaseUniRule annotationImported |
Ligand | FADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)Short name: CPRUniRule annotation Short name: P450RUniRule annotation |
Gene namesi | Name:CprImported Synonyms:CCRImported, CPRImported, cprImported, DmCPRImported, Dmel\CG11567Imported, DMRImported, NCPRImported, P450Imported ORF Names:CG11567Imported, Dmel_CG11567Imported |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0015623 Cpr |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 22 – 43 | HelicalUniRule annotationAdd BLAST | 22 |
Keywords - Cellular componenti
Endoplasmic reticulumUniRule annotation, MembraneExpressioni
Gene expression databases
Bgeei | FBgn0015623 Expressed in 45 organ(s), highest expression level in adult organism |
ExpressionAtlasi | M9PCQ1 differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 84 – 228 | Flavodoxin-likeInterPro annotationAdd BLAST | 145 | |
Domaini | 283 – 523 | FAD-binding FR-typeInterPro annotationAdd BLAST | 241 |
Sequence similaritiesi
Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixUniRule annotationPhylogenomic databases
eggNOGi | KOG1158 Eukaryota COG0369 LUCA |
KOi | K00327 |
OrthoDBi | 318396at2759 |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212 NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208 P450R, 1 hit |
PRINTSi | PR00369 FLAVODOXIN PR00371 FPNCR |
SUPFAMi | SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
M9PCQ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASEQTIDGA AAIPSGGGDE PFLGLLDVAL LAVLIGGAAF YFLRSRKKEE
60 70 80 90 100
EPTRSYSIQP TTVCTTSASD NSFIKKLKAS GRSLVVFYGS QTGTGEEFAG
110 120 130 140 150
RLAKEGIRYR LKGMVADPEE CDMEELLQLK DIDNSLAVFC LATYGEGDPT
160 170 180 190 200
DNAMEFYEWI TSGDVDLSGL NYAVFGLGNK TYEHYNKVAI YVDKRLEELG
210 220 230 240 250
ANRVFELGLG DDDANIEDDF ITWKDRFWPA VCDHFGIEGG GEEVLIRQYR
260 270 280 290 300
LLEQPDVQPD RIYTGEIARL HSIQNQRPPF DAKNPFLAPI KVNRELHKGG
310 320 330 340 350
GRSCMHIELS IEGSKMRYDA GDHVAMFPVN DKSLVEKLGQ LCNADLDTVF
360 370 380 390 400
SLINTDTDSS KKHPFPCPTT YRTALTHYLE ITAIPRTHIL KELAEYCTDE
410 420 430 440 450
KEKELLRSMA SISPEGKEKY QSWIQDACRN IVHILEDIKS CRPPIDHVCE
460 470 480 490 500
LLPRLQPRYY SISSSAKLHP TDVHVTAVLV EYKTPTGRIN KGVATTYLKN
510 520 530 540 550
KQPQGSEEVK VPVFIRKSQF RLPTKPETPI IMVGPGTGLA PFRGFIQERQ
560 570 580 590 600
FLRDEGKTVG ESILYFGCRK RSEDYIYESE LEEWVKKGTL NLKAAFSRDQ
610 620 630 640 650
GKKVYVQHLL EQDADLIWNV IGENKGHFYI CGDAKNMAVD VRNILVKILS
660 670
TKGNMSEADA VQYIKKMEAQ KRYSADVWS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ27597 | NCPR_DROME | NADPH--cytochrome P450 reductase | Cpr CG11567 | 679 | Annotation score: | ||
Q8IPJ7 | Q8IPJ7_DROME | Cytochrome P450 reductase, isoform ... | Cpr CCR, CPR, cpr, DmCPR, Dmel\CG11567 | 550 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014134 Genomic DNA Translation: AGB92664.1 AE014134 Genomic DNA Translation: AGB92665.1 |
RefSeqi | NP_001260128.1, NM_001273199.1 NP_001260129.1, NM_001273200.1 NP_477158.1, NM_057810.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0079250; FBpp0078880; FBgn0015623 FBtr0331223; FBpp0303665; FBgn0015623 FBtr0331224; FBpp0303666; FBgn0015623 |
GeneIDi | 33883 |
KEGGi | dme:Dmel_CG11567 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014134 Genomic DNA Translation: AGB92664.1 AE014134 Genomic DNA Translation: AGB92665.1 |
RefSeqi | NP_001260128.1, NM_001273199.1 NP_001260129.1, NM_001273200.1 NP_477158.1, NM_057810.4 |
3D structure databases
SMRi | M9PCQ1 |
ModBasei | Search... |
Proteomic databases
PRIDEi | M9PCQ1 |
Genome annotation databases
EnsemblMetazoai | FBtr0079250; FBpp0078880; FBgn0015623 FBtr0331223; FBpp0303665; FBgn0015623 FBtr0331224; FBpp0303666; FBgn0015623 |
GeneIDi | 33883 |
KEGGi | dme:Dmel_CG11567 |
Organism-specific databases
CTDi | 33883 |
FlyBasei | FBgn0015623 Cpr |
Phylogenomic databases
eggNOGi | KOG1158 Eukaryota COG0369 LUCA |
KOi | K00327 |
OrthoDBi | 318396at2759 |
Miscellaneous databases
GenomeRNAii | 33883 |
Gene expression databases
Bgeei | FBgn0015623 Expressed in 45 organ(s), highest expression level in adult organism |
ExpressionAtlasi | M9PCQ1 differential |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212 NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208 P450R, 1 hit |
PRINTSi | PR00369 FLAVODOXIN PR00371 FPNCR |
SUPFAMi | SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | M9PCQ1_DROME | |
Accessioni | M9PCQ1Primary (citable) accession number: M9PCQ1 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | June 26, 2013 |
Last sequence update: | June 26, 2013 | |
Last modified: | October 16, 2019 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |