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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Rhodanobacter denitrificans
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotationSAAS annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Zinc 1UniRule annotation1
Metal bindingi55Zinc 1UniRule annotation1
Metal bindingi57Zinc 2UniRule annotation1
Metal bindingi58Zinc 2UniRule annotation1
Metal bindingi111Zinc 1UniRule annotation1
Metal bindingi128Zinc 1UniRule annotation1
Metal bindingi128Zinc 2UniRule annotation1
Metal bindingi166Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
LigandMetal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciRDEN666685:G1H0R-2235-MONOMER
UniPathwayiUPA00619; UER00676

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
ORF Names:R2APBS1_2260Imported
OrganismiRhodanobacter denitrificansImported
Taxonomic identifieri666685 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesRhodanobacteraceaeRhodanobacter
Proteomesi
  • UP000011859 Componenti: Chromosome

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 166Lactamase_BInterPro annotationAdd BLAST156

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01069
OrthoDBiPOG091H03YM

Family and domain databases

CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
Gene3Di3.60.15.10, 1 hit
HAMAPiMF_01374 Glyoxalase_2, 1 hit
InterProiView protein in InterPro
IPR035680 Clx_II_MBL
IPR032282 HAGH_C
IPR017782 Hydroxyacylglutathione_Hdrlase
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF16123 HAGH_C, 1 hit
PF00753 Lactamase_B, 1 hit
PIRSFiPIRSF005457 Glx, 1 hit
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit
TIGRFAMsiTIGR03413 GSH_gloB, 1 hit

Sequencei

Sequence statusi: Complete.

M4NEY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLIPLPALA DNYIWLLHDD HGNAVVVDPG DAAVVEQALV AHRLQLRAIL
60 70 80 90 100
LTHHHHDHIG GVPALRAHHG VPVHAPDDER IGEATARVRD GDRVELAEPS
110 120 130 140 150
VQFEVIAVPG HTLSHVAYVG AGILLCGDTL FSLGCGRLFE GTPAQMLASL
160 170 180 190 200
DRLAQLPGDT LVCGGHEYTE ANGRFARTIE PANADLQQRL QEVAALRARL
210 220 230 240 250
EPTLPVLLAG ERATNPFLRV DTDAVIAWCE RQGAGNDRVA RFAAVRRAKD

GFRA
Length:254
Mass (Da):27,435
Last modified:May 29, 2013 - v1
Checksum:i58B3F8C71225EDB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003470 Genomic DNA Translation: AGG89364.1
RefSeqiWP_015448009.1, NZ_AJXV01000030.1

Genome annotation databases

EnsemblBacteriaiAGG89364; AGG89364; R2APBS1_2260
GeneIDi31836716
KEGGirhd:R2APBS1_2260

Similar proteinsi

Entry informationi

Entry nameiM4NEY0_9GAMM
AccessioniPrimary (citable) accession number: M4NEY0
Entry historyiIntegrated into UniProtKB/TrEMBL: May 29, 2013
Last sequence update: May 29, 2013
Last modified: June 20, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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