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Protein

Biotin synthase

Gene

bioB

Organism
Bacillus subtilis XF-1
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi74Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi111Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi143Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi203Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi273Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotationImported
ORF Names:C663_2865Imported
OrganismiBacillus subtilis XF-1Imported
Taxonomic identifieri1233100 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000011821 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliM4KWB5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 265Elp3InterPro annotationAdd BLAST209
Domaini233 – 323BATSInterPro annotationAdd BLAST91

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01012

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

M4KWB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMNQWMELA DRVLSGAEVT DEEALSILHC PDEDILLLMH GAFHIRKHFY
60 70 80 90 100
GKKVKLNMIM NAKSGLCPEN CGYCSQSAIS KAPIESYRMV NKETLLEGAK
110 120 130 140 150
RAHDLNIGTY CIVASGRGPS NREVDQVVDA VQEIKETYGL KVCACLGLLK
160 170 180 190 200
PEQAKRLKDA GVDRYNHNLN TSQRNHSNIT TSHTYDDRVN TVEIAKESGL
210 220 230 240 250
SPCSGAIIGM KETKQDVIDI AKSLKALDAD SIPVNFLHAI DGTPLEGVNE
260 270 280 290 300
LNPLYCLKVL ALFRFINPSK EIRISGGREV NLRSLQPLGL YAANSIFVGD
310 320 330
YLTTAGQEET EDHKMLSDLG FEVESVEEMK SSLSAKS
Length:337
Mass (Da):37,218
Last modified:May 29, 2013 - v1
Checksum:i44CF90CF6A932B67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP004019 Genomic DNA Translation: AGE64615.1

Genome annotation databases

EnsemblBacteriaiAGE64615; AGE64615; C663_2865
KEGGibsx:C663_2865
PATRICifig|1233100.3.peg.3014

Similar proteinsi

Entry informationi

Entry nameiM4KWB5_BACIU
AccessioniPrimary (citable) accession number: M4KWB5
Entry historyiIntegrated into UniProtKB/TrEMBL: May 29, 2013
Last sequence update: May 29, 2013
Last modified: November 22, 2017
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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