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UniProtKB - M4HYC6 (TPSD2_PINCO)
Protein
Bifunctional levopimaradiene synthase, chloroplastic
Gene
TPS-LAS2
Organism
Pinus contorta (Shore pine) (Lodgepole pine)
Status
Functioni
Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to levopimaradiene. Levopimaradiene is the major products of the enzyme with abietadiene and neoabietadiene. No activity with farnesyl diphosphate (FPP) as substrate.
1 PublicationCatalytic activityi
- EC:5.5.1.121 Publication
- EC:4.2.3.181 Publication
- EC:4.2.3.321 Publication
- EC:4.2.3.1321 Publication
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
: oleoresin biosynthesis Pathwayi
This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 250 | SubstrateBy similarity | 1 | |
Metal bindingi | 383 | Magnesium 4By similarity | 1 | |
Metal bindingi | 385 | Magnesium 4By similarity | 1 | |
Binding sitei | 470 | SubstrateBy similarity | 1 | |
Metal bindingi | 602 | Magnesium 1By similarity | 1 | |
Metal bindingi | 602 | Magnesium 2By similarity | 1 | |
Metal bindingi | 606 | Magnesium 1By similarity | 1 | |
Metal bindingi | 606 | Magnesium 2By similarity | 1 | |
Metal bindingi | 746 | Magnesium 3By similarity | 1 | |
Metal bindingi | 750 | Magnesium 3By similarity | 1 | |
Metal bindingi | 754 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- abietadiene synthase activity Source: UniProtKB-EC
- copalyl diphosphate synthase activity Source: UniProtKB-EC
- levopimaradiene synthase activity Source: UniProtKB-EC
- magnesium ion binding Source: InterPro
- terpene synthase activity Source: InterPro
GO - Biological processi
- defense response Source: UniProtKB-KW
- diterpenoid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme |
Biological process | Plant defense |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00924 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional levopimaradiene synthase, chloroplastic1 PublicationShort name: PcLAS21 Publication Alternative name(s): Diterpene synthase Including the following 2 domains: |
Gene namesi | Name:TPS-LAS21 Publication |
Organismi | Pinus contorta (Shore pine) (Lodgepole pine)Imported |
Taxonomic identifieri | 3339 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Pinopsida › Pinidae › Conifers I › Pinaceae › Pinus › Pinus subgen. Pinus |
Subcellular locationi
Chloroplast
- chloroplast Sequence analysis
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 52 | ChloroplastSequence analysisAdd BLAST | 52 | |
ChainiPRO_0000431417 | 53 – 850 | Bifunctional levopimaradiene synthase, chloroplasticAdd BLAST | 798 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 383 – 386 | DXDD motifCurated | 4 | |
Motifi | 602 – 606 | DDXXD motifCurated | 5 |
Domaini
The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.Curated
Sequence similaritiesi
Keywords - Domaini
Transit peptideFamily and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
M4HYC6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALPSSSLSS QIHTGATTQC IPHFHGSLNA GTSAGKRRSL YLRWGKGPSK
60 70 80 90 100
IVACAGQDPF SVPTLVKREF PPGFWKDHVI ESLMPSYKVA PSDEKRIETL
110 120 130 140 150
ITEIKNMFRS MGYGETNPSA YDTAWVARIP AVDGSEKPQF PETLEWILQN
160 170 180 190 200
QLKDGSWGEE FYFLAYDRIL ATLACIITLT IWQTGDTQVQ KGIEFFKTQA
210 220 230 240 250
GKIEEEADSH RPSGFEIVFP AMLKEAKALG LALPYELPFI QQIIEKREAK
260 270 280 290 300
LQRLPSDLLY ALPTTLLYSL EGLQEIVDWE KIMKLQSKDR SFLSSPSSTA
310 320 330 340 350
AVFMRTGNKK CLEFLNFVLK KFGNHVPCHY PLDLFERLWA VDTVERLGID
360 370 380 390 400
HHFKEEIKDA LDYVYSHWDE RGIGWARENP VPDIDDTAMG LRILRLHGYN
410 420 430 440 450
VSSDVLKTFR DENGEFFCFL GQTQRGVTDM LNVNRCSHVA FPGETIMEEA
460 470 480 490 500
KLCTERYLRN ALEDTGAFDK WALKKNIRGE VEYALKYPWH RSMPRLEARS
510 520 530 540 550
YIENYGPNDV WLGKTMYMMP NISNEKYLEL AKLDFNRVQF FHRQELQDIR
560 570 580 590 600
RWWNSSGFSQ LGFTRERVAE IYFSPASFLF EPEFATCRAV YTKTSNFTVI
610 620 630 640 650
LDDLYDAHGT LDNLKLFSES VKRWDLSLVD QMPQDMKICF KGFYNTFNEI
660 670 680 690 700
AEEGRKRQGR DVLSYIQKVW EVQLEAYTKE AEWSAVRYVP SYDEYIENAS
710 720 730 740 750
VSIALGTVVL ISALFTGEIL TDDILSKIGR DSRFLYLMGL TGRLVNDTKT
760 770 780 790 800
YQAERGQGEV ASAVQCYMKD HPEISEEEAL KHVYTIMDNA LDELNREFVN
810 820 830 840 850
NRDVPDTCRR LVFETARIMQ LFYMDGDGLT LSHNMEIKEH VKNCLFQPVA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JQ240311 mRNA Translation: AFU73863.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JQ240311 mRNA Translation: AFU73863.1 |
3D structure databases
SMRi | M4HYC6 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00924 |
Family and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPSD2_PINCO | |
Accessioni | M4HYC6Primary (citable) accession number: M4HYC6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 7, 2015 |
Last sequence update: | May 29, 2013 | |
Last modified: | September 29, 2021 | |
This is version 29 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families