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Protein
Submitted name:

Uncharacterized protein

Gene
N/A
Organism
Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Pectin + n H2O = n methanol + pectate.SAAS annotation

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.SAAS annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Uncharacterized protein, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Ribosome biogenesis protein BOP1 homolog, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Uncharacterized protein, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase
  2. Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase, Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esteraseSAAS annotation, Hydrolase

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Submitted name:
Uncharacterized proteinImported
OrganismiBrassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis)Imported
Taxonomic identifieri51351 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica
Proteomesi
  • UP000011750 Componenti: Chromosome A09

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_501111038533 – 522Sequence analysisAdd BLAST490

Interactioni

Protein-protein interaction databases

STRINGi3711.Bra036280.1-P

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 171PMEIInterPro annotationAdd BLAST143

Sequence similaritiesi

In the C-terminal section; belongs to the pectinesterase family.SAAS annotation
In the N-terminal section; belongs to the PMEI family.SAAS annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

InParanoidiM4F5C4
OMAiHLIFLIP
OrthoDBiEOG0936088S

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit

Sequencei

Sequence statusi: Complete.

M4F5C4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHNPSRKTSK HINLNLLALT FHLIFLIPTV HSRSTQRLAE PNNITELIVA
60 70 80 90 100
TLNQTILNVN VSYTTFYNLQ KRLGPNIARR YRCAFEDCLG LLDDTIFDLE
110 120 130 140 150
TAISKLQTSS LGAHDVNMLL SDAMTNQDTC LEGFKTSGIH EKNSDNTYKL
160 170 180 190 200
TDSLKDSILK ISSNLSNSLG MLQKIPGHEL SPEAYEVDVE FPSWVLENDK
210 220 230 240 250
RRLHAPVEKT KFNLMVAQDG TGNFTTINDA VSAAPTSSVT RFMIYIKRGV
260 270 280 290 300
YFENVEIPKN KTIIMFVGDG IGRTVIKANR RKGNLGTFQT ATVGVKGEGF
310 320 330 340 350
IAKDISFVNF AGPSPQAVAL RSGSDHSAFY RCSFEGYQDT LYVYSGKQFY
360 370 380 390 400
RECDIYGTVD FICGNAAAVF QNCSLFARKP NPGQKIVYTA QSRTCPNQST
410 420 430 440 450
GISMINCRFL AAPDLIPVKG SFEAYLGRPW KNFSRTIIMK SFIDDLVVPA
460 470 480 490 500
GWLEWNGNFA LETLHYGEYM NEGPGSNITN RVKWPGYRPI LNETEATQFT
510 520
VGPFIDGGTW LNSTGIPFNT GL
Length:522
Mass (Da):58,096
Last modified:May 1, 2013 - v1
Checksum:i43206B202F514E3B
GO

Genome annotation databases

EnsemblPlantsiBra036280.1; Bra036280.1-P; Bra036280
GrameneiBra036280.1; Bra036280.1-P; Bra036280

Similar proteinsi

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3711.Bra036280.1-P

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiBra036280.1; Bra036280.1-P; Bra036280
GrameneiBra036280.1; Bra036280.1-P; Bra036280

Phylogenomic databases

InParanoidiM4F5C4
OMAiHLIFLIP
OrthoDBiEOG0936088S

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiM4F5C4_BRARP
AccessioniPrimary (citable) accession number: M4F5C4
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 2013
Last sequence update: May 1, 2013
Last modified: September 12, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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