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Protein

Kynurenine formamidase

Gene

AFMID

Organism
Mustela putorius furo (European domestic ferret) (Mustela furo)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (TDO2)
  2. Kynurenine formamidase (AFMID)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164NucleophileUniRule annotation1
Active sitei249UniRule annotation1
Active sitei281UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processTryptophan catabolismUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

Protein family/group databases

ESTHERimuspf-m3yp96 Kynurenine-formamidase

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:AFMIDUniRule annotationImported
OrganismiMustela putorius furo (European domestic ferret) (Mustela furo)Imported
Taxonomic identifieri9669 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaMustelidaeMustelinaeMustela
Proteomesi
  • UP000000715 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi9669.ENSMPUP00000013153

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 281Abhydrolase_3InterPro annotationAdd BLAST190

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 99HGGXWUniRule annotation5

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4627 Eukaryota
COG0657 LUCA
GeneTreeiENSGT00390000011093
InParanoidiM3YP96
OMAiKEESGFM
OrthoDBiEOG091G0KT8

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

M3YP96-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEVPGRATGA EAPWKQLSAE ELENQYCPSR WVIRRGAEET LKMYTHLGDK
60 70 80 90 100
ATKKVRATRK SLLHVPYGDG EGEKLDIYFP GEVAAEGLPF CLFLHGGYWQ
110 120 130 140 150
SGSKDMSAFM VEPLTARGVA VVVVAYDIAP KGTLDQMIDQ VTRSIAFVQR
160 170 180 190 200
KYPCNEGLYL CGHSAGAHLA AMMLLANWTK HGVTPNLKGF FLVSGIYDLE
210 220 230 240 250
PIVHTTQNAP LLLTPEDARR NSPQLLLEAA LARPSGPACR VLVTVGQHDS
260 270 280 290 300
PEFHRQSREF FQTLCRGGWD ASFEEIPDVD HFEIIWSLTQ QDFVLTQMIL

STILREL
Length:307
Mass (Da):34,217
Last modified:May 1, 2013 - v1
Checksum:i591D123FD7A420F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AEYP01025192 Genomic DNA No translation available.
RefSeqiXP_012909827.1, XM_013054373.1

Genome annotation databases

EnsembliENSMPUT00000013364; ENSMPUP00000013153; ENSMPUG00000013251
GeneIDi101694456

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AEYP01025192 Genomic DNA No translation available.
RefSeqiXP_012909827.1, XM_013054373.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9669.ENSMPUP00000013153

Protein family/group databases

ESTHERimuspf-m3yp96 Kynurenine-formamidase

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMPUT00000013364; ENSMPUP00000013153; ENSMPUG00000013251
GeneIDi101694456

Organism-specific databases

CTDi125061

Phylogenomic databases

eggNOGiKOG4627 Eukaryota
COG0657 LUCA
GeneTreeiENSGT00390000011093
InParanoidiM3YP96
OMAiKEESGFM
OrthoDBiEOG091G0KT8

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiM3YP96_MUSPF
AccessioniPrimary (citable) accession number: M3YP96
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 2013
Last sequence update: May 1, 2013
Last modified: March 28, 2018
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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