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Protein

Ras-related protein Rab-5A

Gene

Rab5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.By similarity

Activity regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35GTPBy similarity9
Nucleotide bindingi46 – 52GTPBy similarity7
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4
Nucleotide bindingi163 – 165GTPBy similarity3

GO - Molecular functioni

  • GDP-dissociation inhibitor binding Source: RGD
  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processEndocytosis, Phagocytosis, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-5A
Alternative name(s):
Small GTP-binding protein rab51 Publication
Gene namesi
Name:Rab5aImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620936 Rab5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004305011 – 215Ras-related protein Rab-5AAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi212S-geranylgeranyl cysteineBy similarity1
Lipidationi213S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiM0RC99
PRIDEiM0RC99

Interactioni

Subunit structurei

Interacts with GDI1; this promotes dissociation from membranes. Interacts with EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1. Interacts with RABEP1; one RABEP1 homodimer binds two RAB5A chains, but at opposite sides of the dimer. Interacts with SGSM1, SGSM3 and PIK3CB. Interacts with RINL. May be a component of a complex composed of RAB5A, DYN2 and PIK3C3. Does not interact with the BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249171, 1 interactor
IntActiM0RC99, 3 interactors
MINTiM0RC99
STRINGi10116.ENSRNOP00000067210

Structurei

3D structure databases

SMRiM0RC99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 57Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092 Eukaryota
ENOG410YCCP LUCA
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiM0RC99
KOiK07887
TreeFamiTF300199

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

M0RC99-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE
60 70 80 90 100
STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
110 120 130 140 150
DITNEESFSR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS
160 170 180 190 200
YADDNSLLFM ETSAKTPMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD
210
LTEPAQPARS QCCSN
Length:215
Mass (Da):23,625
Last modified:April 3, 2013 - v1
Checksum:i716AB5CC4ECAFD77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167P → S in AAC26004 (Ref. 1) Curated1
Sequence conflicti167P → S in EDL82849 (Ref. 3) Curated1
Sequence conflicti167P → S in AAI61848 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072935 mRNA Translation: AAC26004.1
AABR06079878 Genomic DNA No translation available.
CH474184 Genomic DNA Translation: EDL82849.1
BC161848 mRNA Translation: AAI61848.1
RefSeqiNP_073183.1, NM_022692.1
XP_003751425.1, XM_003751377.4
XP_003752788.1, XM_003752740.4
UniGeneiRn.44477

Genome annotation databases

GeneIDi100361891
64633
KEGGirno:100361891
rno:64633

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072935 mRNA Translation: AAC26004.1
AABR06079878 Genomic DNA No translation available.
CH474184 Genomic DNA Translation: EDL82849.1
BC161848 mRNA Translation: AAI61848.1
RefSeqiNP_073183.1, NM_022692.1
XP_003751425.1, XM_003751377.4
XP_003752788.1, XM_003752740.4
UniGeneiRn.44477

3D structure databases

SMRiM0RC99
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249171, 1 interactor
IntActiM0RC99, 3 interactors
MINTiM0RC99
STRINGi10116.ENSRNOP00000067210

Proteomic databases

PaxDbiM0RC99
PRIDEiM0RC99

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100361891
64633
KEGGirno:100361891
rno:64633

Organism-specific databases

CTDi100361891
5868
RGDi620936 Rab5a

Phylogenomic databases

eggNOGiKOG0092 Eukaryota
ENOG410YCCP LUCA
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiM0RC99
KOiK07887
TreeFamiTF300199

Miscellaneous databases

PROiPR:M0RC99

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB5A_RAT
AccessioniPrimary (citable) accession number: M0RC99
Secondary accession number(s): O88565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: April 3, 2013
Last modified: November 7, 2018
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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