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Protein

Alpha-conotoxin LvIA

Gene
N/A
Organism
Conus lividus (Livid cone)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks alpha-3-beta-2/CHRNA3-CHRNB2 nAChR with high selectivity (IC50=8.67 nM (on rat) and 17.5 (on human)) (PubMed:24398291). Has also weaker activity on alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3-CHRNB2-CHRNB3) (IC50=108 nM (on rat)), alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC50=121 nM (on rat)), alpha-3-beta-4 (CHRNA3-CHRNB4) (IC50=148 nM (on rat)), and alpha-7/CHRNA7 nAChRs (IC50=3000 nM (on rat)) (PubMed:24398291). When tested on mouse with hot-plate tests, this toxin significantly increases the base pain threshold and shows analgesic effects (PubMed:26742048).2 Publications

Miscellaneous

Does not block alpha-9-alpha-10/CHRNA9-CHRNA10, alpha-2-beta-2/CHRNA2-CHRNB2, alpha-2-beta-4/CHRNA2-CHRNB4, alpha-3-beta-4/CHRNA3-CHRNB4, alpha-4-beta-2/CHRNA4-CHRNB2, alpha-4-beta-4/CHRNA4-CHRNB4, alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3-CHRNB2-CHRNB3), alpha-6-beta-4/CHRNA6-CHRNB4, and alpha-1-beta-1-epsilon-delta/CHRNA1-CHRNB1-CHRNE-CHRND.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31May play a crucial role for the selectivity for alpha-3-beta-2 nAChR1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin LvIA2 Publications
Short name:
Alpha-CTx LvIA2 Publications
OrganismiConus lividus (Livid cone)
Taxonomic identifieri89426 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaNeogastropodaConoideaConidaeConusLividoconus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29 – 30NV → RA: Shows inhibition on alpha-3-beta-2 (IC(50)=3.3 nM), beta-3-alpha-6-beta-2-alpha-4-beta-2 (IC(50)=11.4 nM), alpha-6/alpha-3-beta-2-beta-3 (IC(50)=13.5 nM), alpha-4-beta-2 (IC(50)=195 nM), alpha-6/alpha-3-beta-4 (IC(50)=1000 nM) nAChRs. Does not inhibit alpha-3-beta-4, beta-4-alpha-3-beta-4-alpha-3-alpha-5, and alpha-4-beta-4 nAChRs. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000430172‹1 – 20By similarityAdd BLAST›20
PeptideiPRO_000043017321 – 36Alpha-conotoxin LvIA1 PublicationAdd BLAST16

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 28Combined sources1 Publication
Disulfide bondi23 ↔ 36Combined sources1 Publication
Modified residuei36Cysteine amide1 Publication1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.Curated

Structurei

Secondary structure

137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiL8BU87
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.Curated

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Family and domain databases

InterProiView protein in InterPro
IPR009958 Conotoxin_a-typ
IPR018072 Conotoxin_a-typ_CS
PfamiView protein in Pfam
PF07365 Toxin_8, 1 hit
PROSITEiView protein in PROSITE
PS60014 ALPHA_CONOTOXIN, 1 hit

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

L8BU87-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30 
FRGRDAAAKA SGLVGLTDRR GCCSHPACNV DHPEICG
Length:37
Mass (Da):3,841
Last modified:April 3, 2013 - v1
Checksum:iB5501D068E48873C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HF566436 Genomic DNA Translation: CCP46959.1

Similar proteinsi

Cross-referencesi

Web resourcesi

Biological Magnetic Resonance Data Bank

synthetic Alpha-conotoxin LvIA

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HF566436 Genomic DNA Translation: CCP46959.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MDQNMR-A21-36[»]
5XGLX-ray3.44C/E/F/H/J21-36[»]
SMRiL8BU87
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR009958 Conotoxin_a-typ
IPR018072 Conotoxin_a-typ_CS
PfamiView protein in Pfam
PF07365 Toxin_8, 1 hit
PROSITEiView protein in PROSITE
PS60014 ALPHA_CONOTOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCA1A_CONLI
AccessioniPrimary (citable) accession number: L8BU87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: April 3, 2013
Last modified: November 7, 2018
This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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