UniProtKB - L8BU87 (CA1A_CONLI)
Protein
Alpha-conotoxin LvIA
Gene
N/A
Organism
Conus lividus (Livid cone)
Status
Functioni
Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks alpha-3-beta-2/CHRNA3-CHRNB2 nAChR with high selectivity (IC50=8.67 nM (on rat) and 17.5 (on human)) (PubMed:24398291). Has also weaker activity on alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3-CHRNB2-CHRNB3) (IC50=108 nM (on rat)), alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC50=121 nM (on rat)), alpha-3-beta-4 (CHRNA3-CHRNB4) (IC50=148 nM (on rat)), and alpha-7/CHRNA7 nAChRs (IC50=3000 nM (on rat)) (PubMed:24398291). When tested on mouse with hot-plate tests, this toxin significantly increases the base pain threshold and shows analgesic effects (PubMed:26742048).2 Publications
Miscellaneous
Does not block alpha-9-alpha-10/CHRNA9-CHRNA10, alpha-2-beta-2/CHRNA2-CHRNB2, alpha-2-beta-4/CHRNA2-CHRNB4, alpha-3-beta-4/CHRNA3-CHRNB4, alpha-4-beta-2/CHRNA4-CHRNB2, alpha-4-beta-4/CHRNA4-CHRNB4, alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3-CHRNB2-CHRNB3), alpha-6-beta-4/CHRNA6-CHRNB4, and alpha-1-beta-1-epsilon-delta/CHRNA1-CHRNB1-CHRNE-CHRND.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 31 | May play a crucial role for the selectivity for alpha-3-beta-2 nAChR1 Publication | 1 |
GO - Molecular functioni
- acetylcholine receptor inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-conotoxin LvIA2 PublicationsShort name: Alpha-CTx LvIA2 Publications |
Organismi | Conus lividus (Livid cone) |
Taxonomic identifieri | 89426 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Lividoconus |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- other organism postsynaptic membrane Source: UniProtKB-KW
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 – 30 | NV → RA: Shows inhibition on alpha-3-beta-2 (IC(50)=3.3 nM), beta-3-alpha-6-beta-2-alpha-4-beta-2 (IC(50)=11.4 nM), alpha-6/alpha-3-beta-2-beta-3 (IC(50)=13.5 nM), alpha-4-beta-2 (IC(50)=195 nM), alpha-6/alpha-3-beta-4 (IC(50)=1000 nM) nAChRs. Does not inhibit alpha-3-beta-4, beta-4-alpha-3-beta-4-alpha-3-alpha-5, and alpha-4-beta-4 nAChRs. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000430172 | ‹1 – 20 | By similarityAdd BLAST | ›20 | |
PeptideiPRO_0000430173 | 21 – 36 | Alpha-conotoxin LvIA1 PublicationAdd BLAST | 16 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 22 ↔ 28 | Combined sources1 Publication | ||
Disulfide bondi | 23 ↔ 36 | Combined sources1 Publication | ||
Modified residuei | 36 | Cysteine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Cleavage on pair of basic residues, Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom duct.Curated
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MDQ | NMR | - | A | 21-36 | [»] | |
5XGL | X-ray | 3.44 | C/E/F/H/J | 21-36 | [»] | |
SMRi | L8BU87 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domaini
The cysteine framework is I (CC-C-C). Alpha4/7 pattern.Curated
Sequence similaritiesi
Belongs to the conotoxin A superfamily.Curated
Family and domain databases
InterProi | View protein in InterPro IPR009958 Conotoxin_a-typ IPR018072 Conotoxin_a-typ_CS |
Pfami | View protein in Pfam PF07365 Toxin_8, 1 hit |
PROSITEi | View protein in PROSITE PS60014 ALPHA_CONOTOXIN, 1 hit |
i Sequence
Sequence statusi: Fragment.
: The displayed sequence is further processed into a mature form. Sequence processingi
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HF566436 Genomic DNA Translation: CCP46959.1 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Biological Magnetic Resonance Data Bank synthetic Alpha-conotoxin LvIA |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HF566436 Genomic DNA Translation: CCP46959.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MDQ | NMR | - | A | 21-36 | [»] | |
5XGL | X-ray | 3.44 | C/E/F/H/J | 21-36 | [»] | |
SMRi | L8BU87 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR009958 Conotoxin_a-typ IPR018072 Conotoxin_a-typ_CS |
Pfami | View protein in Pfam PF07365 Toxin_8, 1 hit |
PROSITEi | View protein in PROSITE PS60014 ALPHA_CONOTOXIN, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | CA1A_CONLI | |
Accessioni | L8BU87Primary (citable) accession number: L8BU87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 3, 2014 |
Last sequence update: | April 3, 2013 | |
Last modified: | November 7, 2018 | |
This is version 21 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references