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UniProtKB - L7X3S1 (MSH_PAPSO)
Protein
Methyltetrahydroprotoberberine 14-monooxygenase
Gene
CYP82N4
Organism
Papaver somniferum (Opium poppy)
Status
Functioni
Catalyzes the conversion of N-methylated protoberberine alkaloids N-methylstylopine and N-methylcanadine into protopine and allocryptopine, respectively, in the biosynthesis of isoquinoline alkaloid sanguinarine.
1 PublicationCatalytic activityi
- (S)-N-methylcanadine + O2 + reduced [NADPH—hemoprotein reductase] = allocryptopine + 2 H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.971 Publication
Cofactori
hemeBy similarity
: Alkaloid biosynthesis Pathwayi
This protein is involved in Alkaloid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in Alkaloid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 468 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- methyltetrahydroprotoberberine 14-monooxygenase activity Source: UniProtKB
GO - Biological processi
- isoquinoline alkaloid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Methyltetrahydroprotoberberine 14-monooxygenase (EC:1.14.14.971 Publication)Alternative name(s): (S)-cis-N-methylstylopine 14-hydroxylase (S)-cis-N-methyltetrahydroprotoberberine-14-hydroxylase Methyltetrahydroprotoberberine 14-hydroxylase N-methylstylopine hydroxylase Short name: MSH |
Gene namesi | Name:CYP82N4 |
Organismi | Papaver somniferum (Opium poppy) |
Taxonomic identifieri | 3469 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Ranunculales › Papaveraceae › Papaveroideae › Papaver |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430260 | 1 – 526 | Methyltetrahydroprotoberberine 14-monooxygenaseAdd BLAST | 526 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
L7X3S1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTESIKTNR PMDLLLQYLQ PISVALVVIA LVWNYGRRNP TKKLAPEASG
60 70 80 90 100
GRPIMGHLHL FNDGELTHRK LGAMADTYGP VFNIRFGSHK TLVVSDWEIV
110 120 130 140 150
KECFTTNDKL FSNRPGTLGI KLMFYDADSV GYAPYGAYWR DLRKISTLKL
160 170 180 190 200
LSNHRIDTIK HLRSSEVESC FESLYSQWGN GEKSGEFAPV RMDSWLGDLT
210 220 230 240 250
FNVVARIVAG KKNFSANGDV GAQRYKAAMD EAMRLMRFFA FSDVIPSLSW
260 270 280 290 300
LDNLRGLVRE MKKCASEIDS IMATWVEEHR VKRNSGGNSQ LEHDFIDVCL
310 320 330 340 350
DIMEHSSLPG DDPDLVVKST CLDMILGGSD TTTVTLTWAM SLLLNHPQVL
360 370 380 390 400
QKAKEELETQ VGKNRQVDDS DIPNLPFIQA IIKETMRLYP AGPLIERRTM
410 420 430 440 450
EDCEVAGYQV PAGTRLLVNV WKMQRDGNVY KGDPLEFRPD RFLTSNADVD
460 470 480 490 500
LKGQHYELIP FGAGRRICPG VSFAVQLMHL VLARLLHEFE ITTVEPETKV
510 520
DMAESGGLLC YKIMPLEVLI KPRLEI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KC154003 mRNA Translation: AGC92398.1 |
Genome annotation databases
KEGGi | ag:AGC92398 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KC154003 mRNA Translation: AGC92398.1 |
3D structure databases
AlphaFoldDBi | L7X3S1 |
SMRi | L7X3S1 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AGC92398 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSH_PAPSO | |
Accessioni | L7X3S1Primary (citable) accession number: L7X3S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 3, 2014 |
Last sequence update: | April 3, 2013 | |
Last modified: | May 25, 2022 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families