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Protein

Kinesin-like protein KIF14

Gene

Kif14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity (PubMed:24949858). Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis (By similarity). During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively (By similarity). Regulates cell growth through regulation of cell cycle progression and cytokinesis. During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1 (By similarity). During late neurogenesis, regulates the cerebellar and cerebral cortex development and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division (PubMed:23308235, PubMed:24931760). Also is required for chromosome congression and alignment during mitotic cell cycle process (By similarity). Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules (By similarity).By similarity3 Publications

Kineticsi

  1. KM=62 µM for ATP1 Publication
  2. KM=33 µM for ATP (in the presence of microtubule)1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi482 – 489ATPPROSITE-ProRule annotationCombined sources8

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMotor protein
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-MMU-5625900 RHO GTPases activate CIT

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein KIF14Curated
    Gene namesi
    Name:Kif141 PublicationImported
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 1

    Organism-specific databases

    MGIiMGI:1098226 Kif14

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    The lag homozygous mutant mice that lack detectable Kif14 expression are indistinguishable from normal littermates at birth. At P10, mutants exhibit an overt ataxic phenotype that increased in severity over time. At P12, lag homozygous mutant mice are unable to stand for 10 s on a narrow platform. Their gait is wide and uncoordinated, and they frequently fell on their backs while walking. These defects in voluntary movement control, posture and balance are accompanied by progressive weakness and failure to gain body weight. By P14, the homozygous mutants in a litter could be identified by their small size and uncontrolled movements. The homozygous mutants all died by P21. The lag homozygous mutant mice also display a flathead, a reduction in brain size, slender optic nerves, and a translucent spinal cord.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004317061 – 1674Kinesin-like protein KIF14Add BLAST1674

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei257PhosphoserineBy similarity1
    Modified residuei262PhosphothreonineBy similarity1
    Modified residuei973PhosphoserineBy similarity1
    Modified residuei1326PhosphoserineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiL0N7N1
    PRIDEiL0N7N1

    PTM databases

    iPTMnetiL0N7N1
    PhosphoSitePlusiL0N7N1

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000041498 Expressed in 28 organ(s), highest expression level in embryonic stem cell
    ExpressionAtlasiL0N7N1 baseline and differential
    GenevisibleiL0N7N1 MM

    Interactioni

    Subunit structurei

    Directly interacts with PRC1 within a complex also containing KIF4A, KIF20A and KIF23; targets to the central spindle. Directly interacts with CIT depending on the activation state of the kinase (stronger interaction with the kinase-dead form); targets to the midbody. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation (By similarity). Interacts with AKT1; the interaction is detected in the plasma membrane upon INS stimulation and promotes AKT1 phosphorylation. Interacts with SVIL; at midbody during cytokinesis. Interacts with RADIL (via PDZ domain); recruits RADIL to the microtubule network restricting RADIL from interaction with activated RAP1A.By similarity

    GO - Molecular functioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000044257

    Structurei

    Secondary structure

    11674
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliL0N7N1
    SMRiL0N7N1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini393 – 736Kinesin motorPROSITE-ProRule annotationAdd BLAST344
    Domaini860 – 911FHAPROSITE-ProRule annotationAdd BLAST52

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 391Required for PRC1-bindingBy similarityAdd BLAST391
    Regioni391 – 772Required for microtubule-binding with high affinity1 PublicationAdd BLAST382
    Regioni936 – 1674Required for CIT-bindingBy similarityAdd BLAST739

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili743 – 826Sequence analysisAdd BLAST84
    Coiled coili961 – 1110Sequence analysisAdd BLAST150

    Domaini

    The kinesin motor domain binds to microtubules with high affinity and has a robust ATPase activity but a very slow motility. The kinesin motor domain protects microtubules from cold depolymerization. Binds to each tubulin heterodimer resulting in a microtubule complexes. Binds at the tubulin intradimer interface, at the crest of the protofilament, and orients slightly toward the next protofilament.1 Publication

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiKOG0245 Eukaryota
    COG5059 LUCA
    GeneTreeiENSGT00930000150817
    KOiK17915
    OMAiQEVSYHL
    OrthoDBiEOG091G0159

    Family and domain databases

    CDDicd00060 FHA, 1 hit
    Gene3Di3.40.850.10, 1 hit
    InterProiView protein in InterPro
    IPR000253 FHA_dom
    IPR027640 Kinesin-like_fam
    IPR032405 Kinesin_assoc
    IPR019821 Kinesin_motor_CS
    IPR001752 Kinesin_motor_dom
    IPR036961 Kinesin_motor_dom_sf
    IPR027417 P-loop_NTPase
    IPR008984 SMAD_FHA_dom_sf
    PANTHERiPTHR24115 PTHR24115, 1 hit
    PfamiView protein in Pfam
    PF00498 FHA, 1 hit
    PF00225 Kinesin, 1 hit
    PF16183 Kinesin_assoc, 1 hit
    PRINTSiPR00380 KINESINHEAVY
    SMARTiView protein in SMART
    SM00240 FHA, 1 hit
    SM00129 KISc, 1 hit
    SUPFAMiSSF49879 SSF49879, 1 hit
    SSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS50006 FHA_DOMAIN, 1 hit
    PS00411 KINESIN_MOTOR_1, 1 hit
    PS50067 KINESIN_MOTOR_2, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

    L0N7N1-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSVHTSHSRH NIGSLEVSSS QKISASSGLV HSSRLELHLK ADMSECENHD
    60 70 80 90 100
    PFVNAGSKTI DINSTYVISA CKKTRETPVT SDPRRLSLQR RATCGDRESS
    110 120 130 140 150
    LLGSELGNRR TADTSLRLQR RHGRADYVGK WETLNPVGGN PGSDSASQAS
    160 170 180 190 200
    RTEAKGVNND TRVLSSVVSV KDSNDTGLTR CKDPGPPVGA SNEKVTVKDT
    210 220 230 240 250
    NSRAPVGSQR QTEAMRSGHL VVQLTESKSD TPVSGGRNSH RGNAGKDTAK
    260 270 280 290 300
    QVGTFGSSDT RTPVKCVLEH RWTPRHDPPP PKSPALSTPK NNGKDIPKHG
    310 320 330 340 350
    STFRSASSES RTPVKCVPEH RWTPRHDLPP PKSPALSTLK NRIASPRVKP
    360 370 380 390 400
    RPKSSLFANK RESSRESTLP PEENSLVQKT FTEPDSLKVE NSQVTVAVRV
    410 420 430 440 450
    RPFSKREKTE KASQVVFTNG EEITVEHPDM KQVYSFIYDV SFWSFDECHP
    460 470 480 490 500
    GYASQTTVYE TLAAPLLDRA FEGYNTCLFA YGQTGSGKSY TMMGLNEEPG
    510 520 530 540 550
    IIPRFCEDLF AQIAKKQTSE VSYHLEMSFF EVYNEKIHDL LVCKGENGQR
    560 570 580 590 600
    KQPLRAREHP VSGPYVEGLS MNVVSSYSDI QSWLELGNKQ RATAATGMND
    610 620 630 640 650
    KSSRSHSVFT LVMTQTKTEV VEGEEHDHRI TSRINLVDLA GSERCSTAHS
    660 670 680 690 700
    SGQRLKEGVS INKSLLTLGK VISALSEQAN GKRVFIPYRE STLTWLLKES
    710 720 730 740 750
    LGGNSKTAMI ATVSPAASNI EETLSTLRYA TQARLIVNIA KVNEDMNAKL
    760 770 780 790 800
    IRELKAEIEK LKAAQRSNRN IDPERYRLCR QEITSLRMKL HQQERDMAEI
    810 820 830 840 850
    QRVWKEKFEQ AEKRKLQETK ELQKAGVTFQ MDNHLPNLVN LNEDPQLSEM
    860 870 880 890 900
    LLYMVKEGVT TVGKHTPSSS HDIQLSGVLI ADDHCTIRNF GGTVSIVPAG
    910 920 930 940 950
    EAKTYVNGTH ISEPTVLHHG DRVVLGGDHY FRFNHPVEVQ KGKKLSSRNN
    960 970 980 990 1000
    LTTSEGPKDF EFAKNELLTA QRSRLEAEIK DAQLKAKEEM MQGIQIAKEM
    1010 1020 1030 1040 1050
    AQQELSSQKA VYERKIQALE AELREESQRK RLEELNNQKA SHKIEELERA
    1060 1070 1080 1090 1100
    KQHLEQEVYV NKRRLEMETL ATKQALEDHR IRHARILEAL EIEKQKIAEE
    1110 1120 1130 1140 1150
    VQMLQENRGN RDKTFTIQPN WNSMKLSTMI QEANAISDKF KKCYIFGRHD
    1160 1170 1180 1190 1200
    ASDKGRSDTS VRVRNLQLGI STFWSLEKFE SKLAAMKELY ESNGGDRDED
    1210 1220 1230 1240 1250
    VFCDPADEWE PDITSTPVSS LSRRRSRSLM KNRRVSGCLH DIHPIQSMQS
    1260 1270 1280 1290 1300
    SHSSGLMEKP STIYSNSSES FLPGICKELI GSSIDFLGQS FDEEKTIADS
    1310 1320 1330 1340 1350
    LINNLLRLHN GVIAISKAHE EQDEESQDNL FSDRAAQALT IQVACAFEQL
    1360 1370 1380 1390 1400
    VVLFKHWLGD FLPCTGSARL EDELRQDIKK LGGYLQLFLQ GCCSDISSMV
    1410 1420 1430 1440 1450
    KEAQNKVMKI IQQAVQCVGQ LAVLKGSKLC VLENSSKVSS TQEFMAALQD
    1460 1470 1480 1490 1500
    GVTSGMKSLL DSGLETAQDL RQDLSRQSAR EEVTKQMKAS TVEWVGSLEN
    1510 1520 1530 1540 1550
    AVAEWRTKSF RTQAQEGSRQ QVSKLLSLAS EFLKLKSCLQ QTVEMIVSAL
    1560 1570 1580 1590 1600
    RGCPSDLHCL RSCTETICSL ARKLHSDFSA HSASAGSCGN ELPRADCEEL
    1610 1620 1630 1640 1650
    ESLAKSLLLC FECGESPGLS KPWESCSSNS KEEQCKSDRA DCGKSGPRRA
    1660 1670
    CEPHGDATPA VSSGDCTPNR IQWV
    Length:1,674
    Mass (Da):186,445
    Last modified:March 6, 2013 - v1
    Checksum:i7A2616CA801C4183
    GO

    Computationally mapped potential isoform sequencesi

    There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    E9Q3T3E9Q3T3_MOUSE
    Kinesin-like protein KIF14
    Kif14
    1,624Annotation score:
    A0A0J9YUN5A0A0J9YUN5_MOUSE
    Kinesin-like protein KIF14
    Kif14
    497Annotation score:
    L0N759L0N759_MOUSE
    Kinesin-like protein KIF14
    Kif14
    69Annotation score:

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB731728 mRNA Translation: BAM74185.1
    AC122127 Genomic DNA No translation available.
    AC126606 Genomic DNA No translation available.
    CCDSiCCDS78693.1
    RefSeqiNP_001274108.1, NM_001287179.2
    XP_006529792.1, XM_006529729.3
    UniGeneiMm.458468

    Genome annotation databases

    EnsembliENSMUST00000189413; ENSMUSP00000139698; ENSMUSG00000041498
    GeneIDi381293
    KEGGimmu:381293
    UCSCiuc007cuv.2 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB731728 mRNA Translation: BAM74185.1
    AC122127 Genomic DNA No translation available.
    AC126606 Genomic DNA No translation available.
    CCDSiCCDS78693.1
    RefSeqiNP_001274108.1, NM_001287179.2
    XP_006529792.1, XM_006529729.3
    UniGeneiMm.458468

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4OZQX-ray2.71A/B390-738[»]
    ProteinModelPortaliL0N7N1
    SMRiL0N7N1
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000044257

    PTM databases

    iPTMnetiL0N7N1
    PhosphoSitePlusiL0N7N1

    Proteomic databases

    PaxDbiL0N7N1
    PRIDEiL0N7N1

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000189413; ENSMUSP00000139698; ENSMUSG00000041498
    GeneIDi381293
    KEGGimmu:381293
    UCSCiuc007cuv.2 mouse

    Organism-specific databases

    CTDi9928
    MGIiMGI:1098226 Kif14

    Phylogenomic databases

    eggNOGiKOG0245 Eukaryota
    COG5059 LUCA
    GeneTreeiENSGT00930000150817
    KOiK17915
    OMAiQEVSYHL
    OrthoDBiEOG091G0159

    Enzyme and pathway databases

    ReactomeiR-MMU-5625900 RHO GTPases activate CIT

    Miscellaneous databases

    ChiTaRSiKif14 mouse
    PROiPR:L0N7N1
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000041498 Expressed in 28 organ(s), highest expression level in embryonic stem cell
    ExpressionAtlasiL0N7N1 baseline and differential
    GenevisibleiL0N7N1 MM

    Family and domain databases

    CDDicd00060 FHA, 1 hit
    Gene3Di3.40.850.10, 1 hit
    InterProiView protein in InterPro
    IPR000253 FHA_dom
    IPR027640 Kinesin-like_fam
    IPR032405 Kinesin_assoc
    IPR019821 Kinesin_motor_CS
    IPR001752 Kinesin_motor_dom
    IPR036961 Kinesin_motor_dom_sf
    IPR027417 P-loop_NTPase
    IPR008984 SMAD_FHA_dom_sf
    PANTHERiPTHR24115 PTHR24115, 1 hit
    PfamiView protein in Pfam
    PF00498 FHA, 1 hit
    PF00225 Kinesin, 1 hit
    PF16183 Kinesin_assoc, 1 hit
    PRINTSiPR00380 KINESINHEAVY
    SMARTiView protein in SMART
    SM00240 FHA, 1 hit
    SM00129 KISc, 1 hit
    SUPFAMiSSF49879 SSF49879, 1 hit
    SSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS50006 FHA_DOMAIN, 1 hit
    PS00411 KINESIN_MOTOR_1, 1 hit
    PS50067 KINESIN_MOTOR_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiKIF14_MOUSE
    AccessioniPrimary (citable) accession number: L0N7N1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
    Last sequence update: March 6, 2013
    Last modified: November 7, 2018
    This is version 45 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
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