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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Calothrix sp. PCC 7507
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotationSAAS annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Zinc 1UniRule annotation1
Metal bindingi56Zinc 1UniRule annotation1
Metal bindingi58Zinc 2UniRule annotation1
Metal bindingi59Zinc 2UniRule annotation1
Metal bindingi113Zinc 1UniRule annotation1
Metal bindingi137Zinc 1UniRule annotation1
Metal bindingi137Zinc 2UniRule annotation1
Metal bindingi175Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
LigandMetal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCSP99598:G1HCH-3688-MONOMER
UniPathwayiUPA00619; UER00676

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
ORF Names:Cal7507_3711Imported
OrganismiCalothrix sp. PCC 7507Imported
Taxonomic identifieri99598 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesRivulariaceaeCalothrix
Proteomesi
  • UP000010390 Componenti: Chromosome

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 175Lactamase_BInterPro annotationAdd BLAST165

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01069
OrthoDBiPOG091H03YM

Family and domain databases

CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
Gene3Di3.60.15.10, 1 hit
HAMAPiMF_01374 Glyoxalase_2, 1 hit
InterProiView protein in InterPro
IPR035680 Clx_II_MBL
IPR032282 HAGH_C
IPR017782 Hydroxyacylglutathione_Hdrlase
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF16123 HAGH_C, 1 hit
PF00753 Lactamase_B, 1 hit
PIRSFiPIRSF005457 Glx, 1 hit
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit
TIGRFAMsiTIGR03413 GSH_gloB, 1 hit

Sequencei

Sequence statusi: Complete.

K9PNI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVIRLKALS DNYIFLLHDC THNIAAVVDP AEAEPVLQHL AELKAELVAI
60 70 80 90 100
FNTHHHHDHV GGNQQLIQKF PQVTVYGGAE DRGRIPGQQV FLQDGSRVQF
110 120 130 140 150
ADRVAEVIFV PGHTRAHIAY YFPPQDVGET GDLFCGDTLF AGGCGRLFEG
160 170 180 190 200
TPSQMVESLN KLRSLPDNTH VWCAHEYTLK NLQFALTVDA GNTDLQERYD
210 220 230 240 250
EVEAYRSRGE ATVPSLLGVE KLTNPFLRWE QPSLQLAVKS NDPVQTFARI

RGLKDKF
Length:257
Mass (Da):28,773
Last modified:March 6, 2013 - v1
Checksum:i6E2F4A00364F5A3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003943 Genomic DNA Translation: AFY34102.1
RefSeqiWP_015129909.1, NC_019682.1

Genome annotation databases

EnsemblBacteriaiAFY34102; AFY34102; Cal7507_3711
KEGGicalo:Cal7507_3711
PATRICifig|99598.3.peg.4201

Similar proteinsi

Entry informationi

Entry nameiK9PNI9_9CYAN
AccessioniPrimary (citable) accession number: K9PNI9
Entry historyiIntegrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: February 28, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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