UniProtKB - K9LG83 (K9LG83_9ORTO)
Hemagglutinin-esterase-fusion glycoprotein
HEF
Functioni
Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell.
ARBA annotationCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 63 | Beta-D-mannoseCombined sources | 1 | |
Binding sitei | 65 | MannoseCombined sources | 1 | |
Binding sitei | 354 | MannoseCombined sources | 1 |
GO - Molecular functioni
- host cell surface receptor binding Source: InterPro
- sialate 4-O-acetylesterase activity Source: UniProtKB-EC
- sialate 9-O-acetylesterase activity Source: UniProtKB-EC
GO - Biological processi
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- fusion of virus membrane with host plasma membrane Source: InterPro
- viral entry into host cell Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Molecular function | HemagglutininARBA annotation, HydrolaseARBA annotation |
Biological process | Fusion of virus membrane with host endosomal membraneARBA annotation, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cellARBA annotation, Viral penetration into host cytoplasm, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: Hemagglutinin-esterase-fusion glycoproteinARBA annotation (EC:3.1.1.53ARBA annotation) |
Gene namesi | Name:HEFImported |
Organismi | Influenza D virus (D/swine/Oklahoma/1334/2011)Imported |
Taxonomic identifieri | 1173138 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Deltainfluenzavirus › |
Proteomesi |
|
Subcellular locationi
- Cell membrane ARBA annotation; Single-pass type I membrane protein ARBA annotation
- Host cell membrane ARBA annotation; Single-pass type I membrane protein ARBA annotation
- Membrane ARBA annotation; Single-pass type I membrane protein ARBA annotation
- Virion membrane ARBA annotation; Single-pass type I membrane protein ARBA annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 635 – 662 | HelicalSequence analysisAdd BLAST | 28 |
Keywords - Cellular componenti
Host cell membraneARBA annotation, Host membrane, Membrane, Viral envelope proteinARBA annotation, VirionPTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 22 | Interchain (with C-593)Combined sources | ||
Glycosylationi | 28 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Disulfide bondi | 122 ↔ 167 | Combined sources | ||
Disulfide bondi | 142 ↔ 191 | Combined sources | ||
Glycosylationi | 146 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Disulfide bondi | 216 ↔ 258 | Combined sources | ||
Disulfide bondi | 235 ↔ 322 | Combined sources | ||
Disulfide bondi | 243 ↔ 295 | Combined sources | ||
Glycosylationi | 346 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Disulfide bondi | 352 ↔ 358 | Combined sources | ||
Disulfide bondi | 394 ↔ 420 | Combined sources | ||
Glycosylationi | 513 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Glycosylationi | 562 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Disulfide bondi | 593 | Interchain (with C-22)Combined sources | ||
Disulfide bondi | 601 ↔ 605 | Combined sources |
Keywords - PTMi
Disulfide bondARBA annotation, GlycoproteinARBA annotationInteractioni
Subunit structurei
Homotrimer of disulfide-linked HEF1-HEF2.
ARBA annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 422 | Hema_esteraseInterPro annotationAdd BLAST | 373 | |
Domaini | 154 – 310 | Hema_HEFGInterPro annotationAdd BLAST | 157 | |
Domaini | 456 – 628 | Hema_stalkInterPro annotationAdd BLAST | 173 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 234 – 236 | Mannose bindingCombined sources | 3 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 526 – 546 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysis, Transmembrane, Transmembrane helixSequence analysisARBA annotationFamily and domain databases
InterProi | View protein in InterPro IPR008980, Capsid_hemagglutn IPR007142, Hemagglutn-estrase_core IPR003860, Hemagglutn-estrase_hemagglutn IPR001364, Hemagglutn_influenz_A/B IPR014831, Hemagglutn_stalk_influenz-C |
Pfami | View protein in Pfam PF03996, Hema_esterase, 1 hit PF02710, Hema_HEFG, 1 hit PF08720, Hema_stalk, 1 hit PF00509, Hemagglutinin, 1 hit |
SUPFAMi | SSF49818, SSF49818, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFLLLATITA ITACQAEREL ICIVQRVNES FSLHSGFGGN VYSMKTEPMT
60 70 80 90 100
GFTNVTKGAS VINQKDWIGF GDSRTDLTND QFPASSDVPL AVAKKFRSLS
110 120 130 140 150
GASLMLSAFG PPGKVDYLYQ GCGKEKVFYE GVNWSPEAGI DCFGSNWTQT
160 170 180 190 200
KKDFYSRIYE AARSSTCMTL VNSLDTKISS TTATAGTASS CSSSWMKSPL
210 220 230 240 250
WYAESSVNPG AKPQVCGTEQ SATFTLPTSF GIYKCNKHVV QLCYFVYENK
260 270 280 290 300
AKFNTFGCGD YYQNYYDGNG NLIGGMDNRV AAYRGIANAG VKIECPSKIL
310 320 330 340 350
NPGTYSIKST PRFLLVPKRS YCFDTDGGYP IQVVQSEWSA SRRSDNATEE
360 370 380 390 400
ACLQTEGCIF IKKTTPYVGE ADDNHGDIEM RQLLSGLGNN DTVCVSQSGY
410 420 430 440 450
TKGETPFVKD YLSPPKYGRC QLKTDSGRIP TLPSGLIIPQ AGTDSLMRTL
460 470 480 490 500
TPATRIFGID DLIFGLLFVG FVAGGVAGGY FWGRSNGGGG GASVSSTQAG
510 520 530 540 550
FDKIGKDIQQ LRNDTNAAIE GFNGRIAHDE QAIKNLAKEI EDARAEALVG
560 570 580 590 600
ELGIIRSLIV ANISMNLKES LYELANQITK RGGGIAQEAG PGCWYVDSEN
610 620 630 640 650
CDASCKEYIF NFNGSATVPT LRPVDTKVVI TSDPYYLGST IALCLLGLVA
660
IAASVGVIWI CCKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JQ922308 Viral cRNA Translation: AFJ19021.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JQ922308 Viral cRNA Translation: AFJ19021.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5E5W | X-ray | 2.40 | A/C | 19-445 | [»] | |
B/D | 456-612 | [»] | ||||
5E62 | X-ray | 2.20 | A/C | 19-445 | [»] | |
B/D | 464-612 | [»] | ||||
5E64 | X-ray | 2.40 | A/C | 19-445 | [»] | |
B/D | 456-621 | [»] | ||||
5E65 | X-ray | 2.20 | A/C | 19-445 | [»] | |
B/D | 456-621 | [»] | ||||
5E66 | X-ray | 3.10 | A | 19-445 | [»] | |
B | 456-621 | [»] | ||||
SMRi | K9LG83 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR008980, Capsid_hemagglutn IPR007142, Hemagglutn-estrase_core IPR003860, Hemagglutn-estrase_hemagglutn IPR001364, Hemagglutn_influenz_A/B IPR014831, Hemagglutn_stalk_influenz-C |
Pfami | View protein in Pfam PF03996, Hema_esterase, 1 hit PF02710, Hema_HEFG, 1 hit PF08720, Hema_stalk, 1 hit PF00509, Hemagglutinin, 1 hit |
SUPFAMi | SSF49818, SSF49818, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | K9LG83_9ORTO | |
Accessioni | K9LG83Primary (citable) accession number: K9LG83 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | March 6, 2013 |
Last sequence update: | March 6, 2013 | |
Last modified: | May 25, 2022 | |
This is version 52 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |