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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Agaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC 10392) (White button mushroom)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotationSAAS annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei127S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei130UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, RNA-binding, Transferase, tRNA-bindingUniRule annotationSAAS annotation
Biological processtRNA processingUniRule annotationSAAS annotation
LigandS-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:AGABI1DRAFT_101424Imported
OrganismiAgaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC 10392) (White button mushroom)Imported
Taxonomic identifieri597362 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus
Proteomesi
  • UP000008493 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 75S-adenosyl-L-methionine bindingUniRule annotation2
Regioni107 – 108S-adenosyl-L-methionine bindingUniRule annotation2
Regioni208 – 210S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotationSAAS annotation

Phylogenomic databases

InParanoidiK5X5G0
KOiK03439
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

K5X5G0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLPQKRLY RQRAHANPFS DHSLRYPAAP NRLDWSTHFP DGEPPQWADI
60 70 80 90 100
GCGFGGLLVQ LAPQYPNVRI LGLEIRVQVT DYVADRIAAL RVAHPSSYNN
110 120 130 140 150
ISVIRANAMK FLPNFFKKHS LSALFFLFPD PHFKSRKHKA RIISPTLLAE
160 170 180 190 200
YAYVLRPGGI IYTITDVKDL HDWMNTHLSN FPLFQPIDED TLRAQGHGPV
210 220 230
LDAVYTSTEE GKKVQRNKGD KWLACFQRIE QPL
Length:233
Mass (Da):26,615
Last modified:January 9, 2013 - v1
Checksum:iDF8A8CAD18603EC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JH971393 Genomic DNA Translation: EKM78167.1
RefSeqiXP_007331479.1, XM_007331417.1

Genome annotation databases

EnsemblFungiiEKM78167; EKM78167; AGABI1DRAFT_101424
GeneIDi18821808
KEGGiabp:AGABI1DRAFT101424

Similar proteinsi

Entry informationi

Entry nameiK5X5G0_AGABU
AccessioniPrimary (citable) accession number: K5X5G0
Entry historyiIntegrated into UniProtKB/TrEMBL: January 9, 2013
Last sequence update: January 9, 2013
Last modified: May 23, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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