UniProtKB - K4REZ6 (ROSB_STRDJ)
8-demethyl-8-aminoriboflavin-5'-phosphate synthase
rosB
Functioni
Involved in the biosynthesis of the riboflavin analog antibiotic roseoflavin (3,8-dimethylamino-riboflavin) (PubMed:27062037).
Catalyzes the site-specific substitution of the C-8 methyl group of riboflavin-5'-phosphate (FMN) by an amino group to yield 8-amino-8-demethylriboflavin 5'-phosphate, via a combined oxidation, decarboxylation and transamination reaction (PubMed:27062037, PubMed:27331868, PubMed:27981706).
The catalysis is initiated by an oxidation step in which the C-8 methyl group on the dimethylbenzene ring of FMN is converted to a formyl group to yield the 8-demethyl-8-formylriboflavin-5'-phosphate (OHC-RP) intermediate (PubMed:27062037).
In the presence of thiamine, the formyl group is oxidized into a carboxyl group to yield the 8-demethyl-8-carboxyriboflavin-5'-phosphate (HO2C-RP) intermediate (PubMed:27062037).
Finally, in the presence of L-glutamate as an amino donor, decarboxylation and aminotransfer occur, resulting in production of 8-demethyl-8-aminoriboflavin-5'-phosphate (PubMed:27062037).
Addition of NAD (but not NADP) to the reaction increases the yield 1.7-fold (PubMed:27062037).
The reaction also proceeds without the addition of any electron acceptor, and it is possible that molecular oxygen serves this role (PubMed:27062037).
3 PublicationsCatalytic activityi
- 3 A + FMN + H2O + L-glutamate + O2 = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + 3 AH2 + CO2 + H+1 Publication2 PublicationsEC:2.6.1.1141 Publication2 Publications
: Antibiotic biosynthesis Pathwayi
This protein is involved in Antibiotic biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in Antibiotic biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 13 | Important to position the amino-group donor glutamate1 Publication | 1 | |
Binding sitei | 240 | FMN1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 13 | FMNCombined sources1 Publication | 3 | |
Nucleotide bindingi | 19 – 21 | FMNCombined sources1 Publication | 3 | |
Nucleotide bindingi | 91 – 94 | FMNCombined sources1 Publication | 4 | |
Nucleotide bindingi | 132 – 136 | FMNCombined sources1 Publication | 5 |
GO - Molecular functioni
- oxidoreductase activity Source: InterPro
- transaminase activity Source: UniProtKB
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB-KW
- antibiotic metabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Antibiotic biosynthesis |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
BRENDAi | 2.6.1.114, 9909 |
Names & Taxonomyi
Protein namesi | Recommended name: 8-demethyl-8-aminoriboflavin-5'-phosphate synthase1 Publication (EC:2.6.1.1141 Publication2 Publications)Short name: AFP synthase1 Publication Alternative name(s): 8-amino-flavin synthase1 Publication |
Gene namesi | Name:rosB1 Publication Synonyms:Orf79891 Publication ORF Names:BN159_7989Imported |
Organismi | Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768) |
Taxonomic identifieri | 1214101 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 | T → A: Loss of synthase activity due to the absence of substrate binding. 1 Publication | 1 | |
Mutagenesisi | 13 | R → A: Loss of synthase activity due to the reduced ability to catalyze the introduction of an amino group and predominantly to synthesize 8-demethyl-8-carboxyriboflavin-5'-phosphate (HO2C-RP) intermediate. 1 Publication | 1 | |
Mutagenesisi | 19 | S → A: Loss of synthase activity due to the absence of substrate binding. 1 Publication | 1 | |
Mutagenesisi | 21 | T → A: Strong decrease of synthase activity. 1 Publication | 1 | |
Mutagenesisi | 53 | Y → A: Loss of synthase activity. 1 Publication | 1 | |
Mutagenesisi | 55 | D → A: Loss of synthase activity due to the absence of substrate binding. 1 Publication | 1 | |
Mutagenesisi | 94 | N → A: Loss of synthase activity due to the absence of substrate binding. 1 Publication | 1 | |
Mutagenesisi | 240 | Y → A: Loss of synthase activity due to the absence of substrate binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000444475 | 1 – 257 | 8-demethyl-8-aminoriboflavin-5'-phosphate synthaseAdd BLAST | 257 |
Interactioni
Subunit structurei
Homotetramer.
2 PublicationsProtein-protein interaction databases
STRINGi | 1214101.BN159_7989 |
Structurei
Secondary structure
3D structure databases
SMRi | K4REZ6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0655, Bacteria |
HOGENOMi | CLU_082329_0_0_11 |
OMAi | TYWVGEA |
OrthoDBi | 796211at2 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR029039, Flavoprotein-like_sf IPR005025, FMN_Rdtase-like |
Pfami | View protein in Pfam PF03358, FMN_red, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MALKALILNT TLRRSPSRSQ TQGLIDKAVP LYEKEGIETE VVRVIDHDIE
60 70 80 90 100
QEYWDDYDDW NAGEKARRED EWPWLLEKIR EADILVIATP ITLNMCTSAA
110 120 130 140 150
HVILEKLNLM DELNGDTKQF PLYNKVAGLL MCGNEDGAHH VAGTVLNNLG
160 170 180 190 200
RLGYSVPPNA AAYWLGPAGT GPGYIEGKGD RHFHTNKLIR FMVANTSHLA
210 220 230 240 250
RMLQETPYTT DLEACAQAAR EESDDVFAIR VNVNTPAIRY KRFQKLGEVK
VEESQLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE971709 Genomic DNA Translation: CCK32368.1 |
RefSeqi | WP_015662694.1, NC_020504.1 |
Genome annotation databases
EnsemblBacteriai | CCK32368; CCK32368; BN159_7989 |
KEGGi | sdv:BN159_7989 |
PATRICi | fig|1214101.3.peg.8085 |
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE971709 Genomic DNA Translation: CCK32368.1 |
RefSeqi | WP_015662694.1, NC_020504.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MJI | X-ray | 2.00 | A | 1-257 | [»] | |
5MLD | X-ray | 1.70 | A/B/C/D/E/F/G/H | 1-257 | [»] | |
SMRi | K4REZ6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 1214101.BN159_7989 |
Genome annotation databases
EnsemblBacteriai | CCK32368; CCK32368; BN159_7989 |
KEGGi | sdv:BN159_7989 |
PATRICi | fig|1214101.3.peg.8085 |
Phylogenomic databases
eggNOGi | COG0655, Bacteria |
HOGENOMi | CLU_082329_0_0_11 |
OMAi | TYWVGEA |
OrthoDBi | 796211at2 |
Enzyme and pathway databases
BRENDAi | 2.6.1.114, 9909 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR029039, Flavoprotein-like_sf IPR005025, FMN_Rdtase-like |
Pfami | View protein in Pfam PF03358, FMN_red, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ROSB_STRDJ | |
Accessioni | K4REZ6Primary (citable) accession number: K4REZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2018 |
Last sequence update: | January 9, 2013 | |
Last modified: | February 23, 2022 | |
This is version 52 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families