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Entry version 50 (07 Apr 2021)
Sequence version 1 (09 Jan 2013)
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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Marburg marburgvirus
Status
Unreviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein predictedi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.UniRule annotationARBA annotation
RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolaseARBA annotation, MethyltransferaseUniRule annotationARBA annotation, Multifunctional enzymeARBA annotation, Nucleotidyltransferase, RNA-directed RNA polymeraseUniRule annotation, Transferase
Biological processmRNA cappingUniRule annotationARBA annotation, mRNA processing, Viral RNA replicationUniRule annotation
LigandATP-bindingUniRule annotationARBA annotation, Nucleotide-binding, S-adenosyl-L-methionineUniRule annotationARBA annotation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA-directed RNA polymerase LUniRule annotation
Short name:
Protein LUniRule annotation
Alternative name(s):
Large structural proteinUniRule annotation
ReplicaseUniRule annotation
TranscriptaseUniRule annotation
Including the following 5 domains:
RNA-directed RNA polymeraseUniRule annotation (EC:2.7.7.48UniRule annotation)
GTP phosphohydrolaseUniRule annotation (EC:3.6.1.-UniRule annotation)
GDP polyribonucleotidyltransferaseUniRule annotation (EC:2.7.7.88UniRule annotation)
Alternative name(s):
PRNTaseUniRule annotation
mRNA (nucleoside-2'-O-)-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
Short name:
N1-2'-O-MTaseUniRule annotation
mRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
Short name:
G-N7-MTaseUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:LImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMarburg marburgvirusImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri11269 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaOrthornaviraeNegarnaviricotaHaploviricotinaMonjiviricetesMononegaviralesFiloviridaeMarburgvirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000148040 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Host cytoplasmUniRule annotationARBA annotation, VirionUniRule annotationARBA annotation

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini628 – 812RdRp catalyticInterPro annotationAdd BLAST185
Domaini1921 – 2118Mononegavirus-type SAM-dependent 2'-O-MTaseInterPro annotationAdd BLAST198

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1761 – 1781DisorderedSequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1761 – 1778PolyampholyteSequence analysisAdd BLAST18

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039736, L_poly_C
IPR026890, Mononeg_mRNAcap
IPR014023, Mononeg_RNA_pol_cat
IPR025786, Mononega_L_MeTrfase
IPR017235, RNA-dir_pol_L_filovirus

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14318, Mononeg_mRNAcap, 1 hit
PF00946, Mononeg_RNA_pol, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF037548, RNA_pol_Filoviridae, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR04198, paramyx_RNAcap, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590, SAM_MT_MNV_L, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

K4N3H7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQHPTQYPDA RLSSPIVLDQ CDLLARSLGL YSHYSHNPKL RNCRIPHHIY
60 70 80 90 100
RLRNSTALKT FLQNCSILTV PFHSIWDHIL TSIQYDAINH VDDFKYLLPS
110 120 130 140 150
ELVKYANWDN EFLKTYLNKI LRLDHVFPAS ARSQCEDFSP KENPYYWGML
160 170 180 190 200
LLVHLSQLAR RIKGQRGSLR SNWKFIGTDL ELFGIADFII FKVPVKTIIR
210 220 230 240 250
NAVSLQASKP GLRVWYRDQN LTPYLCDDEF IVSVASYECF IMIKDVFIER
260 270 280 290 300
YNTWEICARA WLEDSDGADY PPLDVLGELY NYGDQIIAMY LEDGFKLIKH
310 320 330 340 350
LEPLCVSCIQ THGIFTPRKY WFQSQMIKSY YDELCDLNLK LRISDNKAEC
360 370 380 390 400
AQNFIKTIIQ AKLTPQQYCE LFSLQKHWGH PVLYNDVALD KVKKHAQATK
410 420 430 440 450
ILKPKVMFET FCVFKFIVAK NHYHSQGSWY KTTHDLHLTP YLRQHIVSNS
460 470 480 490 500
FPSQAEIYQH LWEWYFVEHE PLFSTKIISD LSIFIKDRAT AVNQECWDSV
510 520 530 540 550
FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILDFAEK LEYLAPSYRN
560 570 580 590 600
FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT
610 620 630 640 650
EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH
660 670 680 690 700
FINYCNRCYG VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT EDNRNNPPDC
710 720 730 740 750
ANAYHYHLGG IEGLQQKLWT CISCAQITLV ELKTKLKLKS SVMGDNQCIT
760 770 780 790 800
TLSLFPIDAP NDYQENEAEL NAARVAVELA ITTGYSGIFL KPEETFVHSG
810 820 830 840 850
FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL ASIGTSFERG
860 870 880 890 900
TSETRHIFPS RWIASFHSML AINLLNQNHL GFPLGFNIDI SCFKKPLTFS
910 920 930 940 950
EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLK NALEFLGKEE
960 970 980 990 1000
LFYILIAKKP GLADASDFVM NPLGLNVPGS REIITFLRQT VRENITITSQ
1010 1020 1030 1040 1050
NRIINSLFHI GSDLEDQKVC EWLLSSNPVM SRFAADIFSR TPSGKRLQVL
1060 1070 1080 1090 1100
GYLEGTRTLL ASRTISLTTE GTMLMKLREL TRNRWKSWFS YIDALDDDLS
1110 1120 1130 1140 1150
ESLEKFTCTV DVANFLRAYS WSDVLKGKKL IGATLPCLLE QFKVKWINLS
1160 1170 1180 1190 1200
EDLREQFNLS PESELTINLL PYDCKELRLG GSNDTELNYV SCALDRKVVQ
1210 1220 1230 1240 1250
KHPSVKRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR
1260 1270 1280 1290 1300
LLWVTQGTAD REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY
1310 1320 1330 1340 1350
GQHSFMANRM SNTSTRAIIS TNTLGKYAGG GQAAIDSNII FQNTINLGVA
1360 1370 1380 1390 1400
VLDIALSLAK LSSASNVTFR LMLNKCCTRH VPSEYLFFDK PLDVDLNKYM
1410 1420 1430 1440 1450
DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD FPTIAAWTLG
1460 1470 1480 1490 1500
ETIVGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE
1510 1520 1530 1540 1550
SLSLSRIKSI KNLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH
1560 1570 1580 1590 1600
HIPLISLMLG GSAGEKSSSD AVRLFLTASY QNFIHNFSCL MKKGQSSLPV
1610 1620 1630 1640 1650
WLYFPSEGQQ LKPILKILQR LSDLLSPDKV QRCKTLADTC CSIDSFWVYP
1660 1670 1680 1690 1700
SKSTRTNHYY ASLNYWRDKA NKVKNTPFSH LINCSFLELS SHTSSVSSNQ
1710 1720 1730 1740 1750
HVTNSKYISH PEEIPEINTR TKLIEYGSTA LQRMDIKMPP SEQNLGENCR
1760 1770 1780 1790 1800
SSKDIRFKGN QKITKHDQRY EKKESSLQQM SPEDNMQTLA YMHNPSPSQT
1810 1820 1830 1840 1850
FIKSIDVHED FDASRVILNS KINNFNLTDG TINTNLLTTP TGTEFLDTSP
1860 1870 1880 1890 1900
LQSSRYSSTP RERSLPSREQ ASYLYVDCSN IPSISLDPGF RNMSDQNQIQ
1910 1920 1930 1940 1950
MLINAYKRDL HACFDSNQFC RFTGVVSSMH YKLYDLLPPG ELRKAICLAE
1960 1970 1980 1990 2000
GEGSGARLLL KWKETDHLFF NTLATDSQQE AEILSGRVIP RMLYNIDRLS
2010 2020 2030 2040 2050
ALLESRRLIL NNLTIQITDI TNPLWLDSVI QYLPEDSDIL TMDAETTKDE
2060 2070 2080 2090 2100
TREQLYKTIV NIWTRTSPNI PKISIIKVFL LDYEGTLFLM RNAIQYYGQV
2110 2120 2130 2140 2150
QLKKPYSSNA KNSEWYLCCS KRRIQRLQID FPDQVGIFLI CKAMSRQRQA
2160 2170 2180 2190 2200
IPYWLKHIEK NYPASLHEFF LTLGFPSLES SFCHRYTIPF SEGKALFHKV
2210 2220 2230 2240 2250
QSYVRQGKQH LHSLMLDYEN NSPLLDLRNH FICSLRGKIT KYYNDILKLN
2260 2270 2280 2290 2300
LVIKAVEKGK NWSQLVETLP NMHSVCIVHV DHECFGCEKR LLLKLDFIRN
2310 2320 2330
TKIAEQKLLN RVIGYILFFP FGLFKSRSLR A
Length:2,331
Mass (Da):267,137
Last modified:January 9, 2013 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD3056D78B1524F7E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
JX458829 Viral cRNA Translation: AFV31170.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX458829 Viral cRNA Translation: AFV31170.1

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Family and domain databases

InterProiView protein in InterPro
IPR039736, L_poly_C
IPR026890, Mononeg_mRNAcap
IPR014023, Mononeg_RNA_pol_cat
IPR025786, Mononega_L_MeTrfase
IPR017235, RNA-dir_pol_L_filovirus
PfamiView protein in Pfam
PF14318, Mononeg_mRNAcap, 1 hit
PF00946, Mononeg_RNA_pol, 1 hit
PIRSFiPIRSF037548, RNA_pol_Filoviridae, 1 hit
TIGRFAMsiTIGR04198, paramyx_RNAcap, 1 hit
PROSITEiView protein in PROSITE
PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590, SAM_MT_MNV_L, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiK4N3H7_9MONO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: K4N3H7
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: January 9, 2013
Last sequence update: January 9, 2013
Last modified: April 7, 2021
This is version 50 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)
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