UniProtKB - K4CJJ1 (NCED2_SOLLC)
Protein
9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic
Gene
NCED2
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid (ABA) biosynthesis from carotenoids.By similarity
Catalytic activityi
- EC:1.13.11.51By similarity
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
: abscisate biosynthesis Pathwayi
This protein is involved in the pathway abscisate biosynthesis, which is part of Plant hormone biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway abscisate biosynthesis and in Plant hormone biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 279 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 328 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 393 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 568 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: UniProtKB-EC
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: UniProtKB-UniPathway
- carotene catabolic process Source: GO_Central
- response to abscisic acid Source: UniProtKB
- response to water deprivation Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.13.11.51, 3101 |
UniPathwayi | UPA00090 |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic1 Publication (EC:1.13.11.51By similarity)Short name: LeNCED21 Publication Short name: SlNCED21 Publication Alternative name(s): Nine-cis-epoxycarotenoid dioxygenase 21 Publication |
Gene namesi | Name:NCED21 Publication Ordered Locus Names:Solyc08g016720.1.1Curated |
Organismi | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
Taxonomic identifieri | 4081 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Solanum subgen. Lycopersicon |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast stroma By similarity
Note: Partially bound to the thylakoid.By similarity
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 82 | ChloroplastSequence analysisAdd BLAST | 82 | |
ChainiPRO_0000445688 | 83 – 581 | 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplasticAdd BLAST | 499 |
Proteomic databases
PaxDbi | K4CJJ1 |
PRIDEi | K4CJJ1 |
Expressioni
Tissue specificityi
Expressed in flowers (PubMed:29632966). Expressed in developing and ripening fruits (PubMed:22345638, PubMed:22108525).3 Publications
Developmental stagei
Expressed in the meristem during gametogenesis and mainly in ovule, stigma, anther/pollen and vascular tissues during flower development. In flower buds, present in petal primordium and apical meristem. Later expressed in stamen primordium and pistil meristem after petal formation. Accumulates in stamen, pistil and vascular tissues of the bud base. Highly expressed in sporogenous cells in anther, pistil stigma, ovules, pollen grains, anther walls, and basal vascular bundle. In anthers, first present at high levels, then declines rapidly from stage 13 (when microspores transform into vacuolated pollen grains after mitotic divisions) to stage 16 (when the sepals are better separated, and the faint yellow petals are clearer) to a very low level (PubMed:29632966). In fruits, expressed at high levels at the immature stage and then declined continually through all stages of fruit development (PubMed:22345638, PubMed:22108525, PubMed:25039074).4 Publications
Inductioni
Induced by abscisic-acid (ABA) and dehydration.1 Publication
Gene expression databases
ExpressionAtlasi | K4CJJ1, baseline and differential |
Family & Domainsi
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | K4CJJ1 |
OMAi | FIWPFEA |
OrthoDBi | 524712at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
K4CJJ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSTIANYRV SHSFSPSTSY SLDFTLPSKS ISMKNHTTTK TKIHSALLTL
60 70 80 90 100
PKQNNTPKNQ PQFQTSHWNF FQKAAAKALD IVESALVSRE LQNPLPKTAD
110 120 130 140 150
PRVQIAGNFA PVPEQSVRHN LPVTGTIPDC INGVYVRNGA NPLFEPVAGH
160 170 180 190 200
HLFDGDGMVH AVTVENGSVS YSCRFTETER LVQERELGHP VFPKAIGELH
210 220 230 240 250
GHSGIARLLL FYARGVFGLV DHSHGTGVAN AGLVFFNNRL LAMSEDDVPY
260 270 280 290 300
HVQVLPSGDL QTVGRYNFDD QLKSTMIAHP KIDPVSGELF ALSYDVVQKP
310 320 330 340 350
YLKSFKFSPD GEKSPDVEIP LDVPTMMHDF AITENYVVIP DQQVVFKLQE
360 370 380 390 400
MIKGGSPVIY DKNKKSRFGI LPKNAENSEN IIWVESAETF CFHLWNAWEE
410 420 430 440 450
PETDEVIVIG SCMTPPDSIF NECNENLKSV LSEIRLNLKT GESTRRQLLS
460 470 480 490 500
PSDQVNLEAG MVNRNKLGRK TQFAYLAIAE PWPKVSGFAK VDLSTGEIKK
510 520 530 540 550
HIYGDKRYGG EPLFLPRNVN SEKEDDGYIL AFCHDEKTWK SELQIVNAMT
560 570 580
LELEATVKLP SRVPYGFHGT FISSKDLQNQ V
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 335 | N → S in ACL00683 (PubMed:19246595).Curated | 1 | |
Sequence conflicti | 449 | L → P in ACL00683 (PubMed:19246595).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM001071 Genomic DNA No translation available. EU912387 mRNA Translation: ACL00683.2 |
RefSeqi | XP_004244807.1, XM_004244759.3 |
Genome annotation databases
EnsemblPlantsi | Solyc08g016720.1.1; Solyc08g016720.1.1.1; Solyc08g016720.1 |
GeneIDi | 100316877 |
Gramenei | Solyc08g016720.1.1; Solyc08g016720.1.1.1; Solyc08g016720.1 |
KEGGi | sly:100316877 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM001071 Genomic DNA No translation available. EU912387 mRNA Translation: ACL00683.2 |
RefSeqi | XP_004244807.1, XM_004244759.3 |
3D structure databases
SMRi | K4CJJ1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4081.Solyc08g016720.1.1 |
Proteomic databases
PaxDbi | K4CJJ1 |
PRIDEi | K4CJJ1 |
Genome annotation databases
EnsemblPlantsi | Solyc08g016720.1.1; Solyc08g016720.1.1.1; Solyc08g016720.1 |
GeneIDi | 100316877 |
Gramenei | Solyc08g016720.1.1; Solyc08g016720.1.1.1; Solyc08g016720.1 |
KEGGi | sly:100316877 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | K4CJJ1 |
OMAi | FIWPFEA |
OrthoDBi | 524712at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00090 |
BRENDAi | 1.13.11.51, 3101 |
Gene expression databases
ExpressionAtlasi | K4CJJ1, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED2_SOLLC | |
Accessioni | K4CJJ1Primary (citable) accession number: K4CJJ1 Secondary accession number(s): C5H805 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 7, 2018 |
Last sequence update: | November 28, 2012 | |
Last modified: | December 2, 2020 | |
This is version 48 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families