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Entry version 72 (29 Sep 2021)
Sequence version 2 (13 Nov 2013)
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Protein

DNA topoisomerase 2

Gene

CNAG_00377

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationARBA annotation EC:5.6.2.2

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-bindingUniRule annotationARBA annotation, Isomerase, TopoisomeraseUniRule annotationARBA annotation
LigandATP-bindingUniRule annotation, MagnesiumARBA annotation, Metal-bindingARBA annotation, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA topoisomerase 2UniRule annotationARBA annotation (EC:5.6.2.2UniRule annotationARBA annotation)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:CNAG_00377Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri235443 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000010091 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:CNAG_00377

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

PTM databases

SwissPalm database of S-palmitoylation events

More...
SwissPalmi
J9VEP2

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

UniRule annotation

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini555 – 669ToprimInterPro annotationAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 125DisorderedSequence analysisAdd BLAST125
Regioni1173 – 1193DisorderedSequence analysisAdd BLAST21
Regioni1260 – 1312DisorderedSequence analysisAdd BLAST53

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili1215 – 1235Sequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1 – 19Acidic residuesSequence analysisAdd BLAST19
Compositional biasi111 – 125Polar residuesSequence analysisAdd BLAST15
Compositional biasi1280 – 1301Basic and acidic residuesSequence analysisAdd BLAST22

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotationARBA annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_001935_1_1_1

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00187, TOP4c, 1 hit
cd03365, TOPRIM_TopoIIA, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.268.10, 1 hit
3.30.230.10, 1 hit
3.30.565.10, 1 hit
3.40.50.670, 1 hit
3.90.199.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003594, HATPase_C
IPR036890, HATPase_C_sf
IPR020568, Ribosomal_S5_D2-typ_fold
IPR014721, Ribosomal_S5_D2-typ_fold_subgr
IPR001241, Topo_IIA
IPR013760, Topo_IIA-like_dom_sf
IPR002205, Topo_IIA_A/C
IPR013758, Topo_IIA_A/C_ab
IPR013757, Topo_IIA_A_a_sf
IPR013759, Topo_IIA_B_C
IPR013506, Topo_IIA_bsu_dom2
IPR018522, TopoIIA_CS
IPR031660, TOPRIM_C
IPR006171, TOPRIM_domain
IPR034157, TOPRIM_TopoII

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00204, DNA_gyraseB, 1 hit
PF00521, DNA_topoisoIV, 1 hit
PF02518, HATPase_c, 1 hit
PF01751, Toprim, 1 hit
PF16898, TOPRIM_C, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00387, HATPase_c, 1 hit
SM00433, TOP2c, 1 hit
SM00434, TOP4c, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF54211, SSF54211, 1 hit
SSF55874, SSF55874, 1 hit
SSF56719, SSF56719, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00177, TOPOISOMERASE_II, 1 hit
PS50880, TOPRIM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

J9VEP2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDSESDDFR IDDDDSESDD YVAPIKAKKA AVAKKAPAAK VPVAKKTPAA
60 70 80 90 100
KAPVTKKAVP KKITKAPLAS KKHPNDSISD ASSDIVCSPP KKKTKADDDD
110 120 130 140 150
DDDDGDTGAE SSAKAPTSNK NASEVYQKLS QREHVLKRPD TYIGSVEAIT
160 170 180 190 200
QPMWVLNPET KTMVHRPITF VPGFLKIFDE ILVNASDNKI NDPTMDSIKV
210 220 230 240 250
VIDREKNTIS VYNNGRGIPV EMHTKEGVMI PELIFGHLLA GSNFDDDQKK
260 270 280 290 300
LTGGRNGYGA KLANIYSHEF IVETADKVNS KKYKQIFSNN MSKKGTPKIL
310 320 330 340 350
ENKKGEEWTK ITFKPDLERF GMNGIDDDTN ALLMKRVYDM AGTVKDVKVF
360 370 380 390 400
LNDERIKVKN FKQYVEMYLN ASTTAASEAA GGAAVTKPPI IYEVANKRWE
410 420 430 440 450
VAFALSDGEF KQVSFTNSIA TTKGGTHVDM IATQIANKLM DQIKKKNKAA
460 470 480 490 500
PVKPFQIKNH MWIFVNALIE NPAFDSQTKE NLTLKSSAFG SKCELSEEFI
510 520 530 540 550
KKVAKTGIID NVLNWARFKQ DQILKKTDGT KRSRISGIVK LEDANNAGGR
560 570 580 590 600
NSKNCTLILT EGDSAKALAV SGLAVVGRDE YGVFPLRGKL LNVREAGHDQ
610 620 630 640 650
IIKNVEIQHI KQILGLKHNQ DYATVDSLRY GHLMIMTDQD HDGSHIKGLI
660 670 680 690 700
INFLDHFYPS LLRIPNFLVE FITPIVKVWK GKKEISFYTM PQYEEWKSQN
710 720 730 740 750
NNGRGWESKY YKGLGTSTSA DAKKYFSDLD KHRLAFETMQ PEDRQLIDMA
760 770 780 790 800
FNKKKADDRK EWLRQFKPGT YLDHDVQSLP ISDFVNKELI LFSMADNIRS
810 820 830 840 850
IPSVADGLKP GQRKVLFGCF KRNLTKEIKV AQLAGYVSEK TAYHHGEASL
860 870 880 890 900
TSTIVGLAQN FVGSNNINLL DPNGQFGTRL AGGKDAASAR YIFTNLPRMT
910 920 930 940 950
RAIFHPADDG LLNYLVEDGM RIEPDYFMPT VPMVLINGAD GIGTGWSTAI
960 970 980 990 1000
PNYNPLDIVE NLRRMMRGEE PEKMTPWFRG FKGSIERIDQ DKYKVSGVIE
1010 1020 1030 1040 1050
KINDTTLEIT ELPIRRWTQD FKEMLEEMTT GTDKVPQTIK DYEEHHTDTT
1060 1070 1080 1090 1100
VHFRVHMTEK NLADAEKEGF DKRFRLTTTL GTGNMVCFDL NGKIKKYASP
1110 1120 1130 1140 1150
EEILEEFFHK RLEYYGHRKQ HLADELNRQF ERLSNQARFV HMIITKELVV
1160 1170 1180 1190 1200
SGKRRPDIVT ELRNLKFRPF PKKEKAKEEG ENGAAEEEEE WDEGQASDYD
1210 1220 1230 1240 1250
YLLGMAIWSL TSEKVEKLLA ERDAKEQELI ELLKLSPNDI WDRDLDNFLA
1260 1270 1280 1290 1300
EWQLALDADT ADAKGSKPKT KAAIKATQKR KKRAAGDDTD DDSDDFKPPA
1310
TVLKPKGRGK RG
Length:1,312
Mass (Da):147,802
Last modified:November 13, 2013 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i206818629A079DF3
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CP003820 Genomic DNA Translation: AFR92513.2
CP003820 Genomic DNA Translation: AGV15299.1

NCBI Reference Sequences

More...
RefSeqi
XP_012046286.1, XM_012190896.1
XP_012046682.1, XM_012191292.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
AFR92513; AFR92513; CNAG_00377
AGV15299; AGV15299; CNAG_00377

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
23884189

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003820 Genomic DNA Translation: AFR92513.2
CP003820 Genomic DNA Translation: AGV15299.1
RefSeqiXP_012046286.1, XM_012190896.1
XP_012046682.1, XM_012191292.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

PTM databases

SwissPalmiJ9VEP2

Genome annotation databases

EnsemblFungiiAFR92513; AFR92513; CNAG_00377
AGV15299; AGV15299; CNAG_00377
GeneIDi23884189

Organism-specific databases

VEuPathDBiFungiDB:CNAG_00377

Phylogenomic databases

HOGENOMiCLU_001935_1_1_1

Family and domain databases

CDDicd00187, TOP4c, 1 hit
cd03365, TOPRIM_TopoIIA, 1 hit
Gene3Di1.10.268.10, 1 hit
3.30.230.10, 1 hit
3.30.565.10, 1 hit
3.40.50.670, 1 hit
3.90.199.10, 1 hit
InterProiView protein in InterPro
IPR003594, HATPase_C
IPR036890, HATPase_C_sf
IPR020568, Ribosomal_S5_D2-typ_fold
IPR014721, Ribosomal_S5_D2-typ_fold_subgr
IPR001241, Topo_IIA
IPR013760, Topo_IIA-like_dom_sf
IPR002205, Topo_IIA_A/C
IPR013758, Topo_IIA_A/C_ab
IPR013757, Topo_IIA_A_a_sf
IPR013759, Topo_IIA_B_C
IPR013506, Topo_IIA_bsu_dom2
IPR018522, TopoIIA_CS
IPR031660, TOPRIM_C
IPR006171, TOPRIM_domain
IPR034157, TOPRIM_TopoII
PfamiView protein in Pfam
PF00204, DNA_gyraseB, 1 hit
PF00521, DNA_topoisoIV, 1 hit
PF02518, HATPase_c, 1 hit
PF01751, Toprim, 1 hit
PF16898, TOPRIM_C, 1 hit
SMARTiView protein in SMART
SM00387, HATPase_c, 1 hit
SM00433, TOP2c, 1 hit
SM00434, TOP4c, 1 hit
SUPFAMiSSF54211, SSF54211, 1 hit
SSF55874, SSF55874, 1 hit
SSF56719, SSF56719, 1 hit
PROSITEiView protein in PROSITE
PS00177, TOPOISOMERASE_II, 1 hit
PS50880, TOPRIM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiJ9VEP2_CRYNH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: J9VEP2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 13, 2013
Last modified: September 29, 2021
This is version 72 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)
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