Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

D-alanine--D-alanine ligase



Escherichia coli
Unreviewed-Annotation score: -Protein inferred from homologyi


Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation


Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.


Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi257Magnesium or manganese 1UniRule annotation1
Metal bindingi270Magnesium or manganese 1UniRule annotation1
Metal bindingi270Magnesium or manganese 2UniRule annotation1
Metal bindingi272Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi


Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell cycle, Cell divisionImported, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases


Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC: annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Synonyms:ddlUniRule annotationImported
ORF Names:A8C65_16265Imported, A9R57_20470Imported, AC789_1c00920Imported, ACN002_0092Imported, ACU57_16065Imported, AM266_01485Imported, AM270_12055Imported, AMK83_01600Imported, AML07_23605Imported, APT94_27415Imported, APZ14_15185Imported, AUQ13_07885Imported, AUS26_14655Imported, AW059_09405Imported, B1K96_13400Imported, BB545_20490Imported, BE963_14430Imported, BET08_06765Imported, BHF46_08275Imported, BHS87_00390Imported, BIQ87_00385Imported, BIU72_13840Imported, BJJ90_21750Imported, BK248_21020Imported, BK292_13285Imported, BK334_15405Imported, BK373_09210Imported, BK400_03995Imported, BMR12_10430Imported, BMT53_09900Imported, BN17_45141Imported, BUE81_03720Imported, BVL39_02595Imported, BW690_22025Imported, BXT93_15680Imported, BZL31_13400Imported, C2M16_10395Imported, C2U48_10020Imported, C4J69_03845Imported, C4K42_02520Imported, C5715_27150Imported, C5N07_08165Imported, C5P43_03105Imported, C6976_00520Imported, C7235_20640Imported, C7B06_05460Imported, C7B07_04685Imported, C7B08_10095Imported, C7B18_05705Imported, C9E25_07300Imported, CA268_07855Imported, CA593_03280Imported, CHQ87_0020115Imported, CIJ94_16040Imported, COD46_06225Imported, CR534_22775Imported, CR538_21050Imported, CR539_04520Imported, CSB64_19500Imported, CU078_10955Imported, CV83915_01485Imported, CXB56_23540Imported, EC3234A_2c00760Imported, ECONIH1_00510Imported, EL75_3670Imported, EL79_3780Imported, EL80_3727Imported, ERS085365_01030Imported, ERS085379_00613Imported, ERS085416_01582Imported, ERS139211_00749Imported, ERS150876_00142Imported, FORC28_5107Imported, GJ11_00490Imported, HMPREF3040_02529Imported, HW43_04190Imported, JD73_13510Imported, MJ49_06580Imported, MS6198_01060Imported, PGD_03733Imported, PU06_15025Imported, RK56_013485Imported, RX35_04829Imported, SK85_00090Imported, UN86_27885Imported, UN91_10970Imported, WQ89_09170Imported, WR15_01945Imported, YDC107_3322Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
  • UP000241864 Componenti: Unassembled WGS sequence
  • UP000240448 Componenti: Unassembled WGS sequence
  • UP000237650 Componenti: Unassembled WGS sequence
  • UP000044893 Componenti: Unassembled WGS sequence
  • UP000230908 Componenti: Chromosome
  • UP000217968 Componenti: Unassembled WGS sequence
  • UP000193530 Componenti: Unassembled WGS sequence
  • UP000194908 Componenti: Unassembled WGS sequence
  • UP000030018 Componenti: Unassembled WGS sequence
  • UP000065978 Componenti: Chromosome
  • UP000030000 Componenti: Unassembled WGS sequence
  • UP000197281 Componenti: Unassembled WGS sequence
  • UP000194247 Componenti: Unassembled WGS sequence
  • UP000188724 Componenti: Unassembled WGS sequence
  • UP000070045 Componenti: Unassembled WGS sequence
  • UP000234324 Componenti: Chromosome
  • UP000037564 Componenti: Unassembled WGS sequence
  • UP000051846 Componenti: Unassembled WGS sequence
  • UP000094504 Componenti: Chromosome
  • UP000215625 Componenti: Unassembled WGS sequence
  • UP000194285 Componenti: Unassembled WGS sequence
  • UP000040934 Componenti: Unassembled WGS sequence
  • UP000070450 Componenti: Unassembled WGS sequence
  • UP000234238 Componenti: Chromosome
  • UP000185422 Componenti: Chromosome
  • UP000185961 Componenti: Unassembled WGS sequence
  • UP000240478 Componenti: Unassembled WGS sequence
  • UP000044469 Componenti: Unassembled WGS sequence
  • UP000222984 Componenti: Unassembled WGS sequence
  • UP000036012 Componenti: Unassembled WGS sequence
  • UP000236587 Componenti: Chromosome
  • UP000028651 Componenti: Unassembled WGS sequence
  • UP000238978 Componenti: Unassembled WGS sequence
  • UP000033371 Componenti: Unassembled WGS sequence
  • UP000186204 Componenti: Unassembled WGS sequence
  • UP000188465 Componenti: Unassembled WGS sequence
  • UP000031522 Componenti: Chromosome
  • UP000029988 Componenti: Unassembled WGS sequence
  • UP000030783 Componenti: Chromosome
  • UP000239819 Componenti: Unassembled WGS sequence
  • UP000054864 Componenti: Unassembled WGS sequence
  • UP000245030 Componenti: Chromosome
  • UP000077380 Componenti: Unassembled WGS sequence
  • UP000036812 Componenti: Unassembled WGS sequence
  • UP000030834 Componenti: Unassembled WGS sequence
  • UP000239287 Componenti: Unassembled WGS sequence
  • UP000194331 Componenti: Chromosome
  • UP000095493 Componenti: Unassembled WGS sequence
  • UP000184168 Componenti: Unassembled WGS sequence
  • UP000218721 Componenti: Unassembled WGS sequence
  • UP000054277 Componenti: Unassembled WGS sequence
  • UP000236496 Componenti: Unassembled WGS sequence
  • UP000215493 Componenti: Unassembled WGS sequence
  • UP000184369 Componenti: Unassembled WGS sequence
  • UP000066343 Componenti: Chromosome
  • UP000236598 Componenti: Unassembled WGS sequence
  • UP000030317 Componenti: Chromosome
  • UP000238802 Componenti: Chromosome
  • UP000076262 Componenti: Unassembled WGS sequence
  • UP000040589 Componenti: Unassembled WGS sequence
  • UP000033592 Componenti: Unassembled WGS sequence
  • UP000234543 Componenti: Chromosome
  • UP000236551 Componenti: Chromosome
  • UP000184459 Componenti: Unassembled WGS sequence
  • UP000094022 Componenti: Unassembled WGS sequence
  • UP000094451 Componenti: Chromosome
  • UP000184336 Componenti: Unassembled WGS sequence
  • UP000238857 Componenti: Unassembled WGS sequence
  • UP000234906 Componenti: Chromosome
  • UP000051850 Componenti: Unassembled WGS sequence
  • UP000189664 Componenti: Chromosome
  • UP000216366 Componenti: Unassembled WGS sequence
  • UP000092566 Componenti: Chromosome
  • UP000184178 Componenti: Unassembled WGS sequence
  • UP000050556 Componenti: Unassembled WGS sequence
  • UP000196822 Componenti: Unassembled WGS sequence
  • UP000076304 Componenti: Unassembled WGS sequence
  • UP000095183 Componenti: Unassembled WGS sequence
  • UP000194199 Componenti: Chromosome
  • UP000188967 Componenti: Unassembled WGS sequence
  • UP000241957 Componenti: Unassembled WGS sequence
  • UP000041959 Componenti: Unassembled WGS sequence
  • UP000240829 Componenti: Unassembled WGS sequence
  • UP000070338 Componenti: Unassembled WGS sequence
  • UP000238965 Componenti: Unassembled WGS sequence
  • UP000216687 Componenti: Unassembled WGS sequence
  • UP000239654 Componenti: Chromosome
  • UP000225731 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation


Protein-protein interaction databases


Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 303ATP-graspInterPro annotationAdd BLAST203

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 2 hits
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit


Sequence statusi: Complete.

J7QWX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
60 70 80 90 100
110 120 130 140 150
160 170 180 190 200
210 220 230 240 250
260 270 280 290 300

Mass (Da):32,840
Last modified:October 31, 2012 - v1

Sequence databases

Select the link destinations:
Links Updated
CP009859 Genomic DNA Translation: AIX61817.1
CP009106 Genomic DNA Translation: AIZ85670.1
CP010315 Genomic DNA Translation: AJE54391.1
CP013025 Genomic DNA Translation: ALL87292.1
CP007491 Genomic DNA Translation: ALY11550.1
CP015229 Genomic DNA Translation: ANO87245.1
CP012693 Genomic DNA Translation: AOM48083.1
CP015834 Genomic DNA Translation: AOM68394.1
CP017631 Genomic DNA Translation: APT04441.1
CP018957 Genomic DNA Translation: AQV30681.1
CP021175 Genomic DNA Translation: ARR32215.1
CP021202 Genomic DNA Translation: ARR59340.1
CP024821 Genomic DNA Translation: ATX09207.1
CP024978 Genomic DNA Translation: ATZ31832.1
CP024147 Genomic DNA Translation: AUJ94752.1
CP024141 Genomic DNA Translation: AUK02699.1
CP024127 Genomic DNA Translation: AUK23634.1
CP019051 Genomic DNA Translation: AUL67125.1
CP025707 Genomic DNA Translation: AUO35473.1
CP026202 Genomic DNA Translation: AUV31047.1
CP027205 Genomic DNA Translation: AVN02721.1
CP029242 Genomic DNA Translation: AWK00876.1
HE964769 Genomic DNA Translation: CCJ42560.1
HG976958 Genomic DNA Translation: CDU40703.1
CYAQ01000006 Genomic DNA Translation: CTS13247.1
CYBF01000003 Genomic DNA Translation: CTT31890.1
CYDH01000006 Genomic DNA Translation: CTV24037.1
CYCX01000010 Genomic DNA Translation: CTV54797.1
CYGC01000001 Genomic DNA Translation: CTZ22945.1
JPQG01000002 Genomic DNA Translation: KFD77273.1
JPKH01000046 Genomic DNA Translation: KGM67917.1
JPKL01000117 Genomic DNA Translation: KGM77191.1
JPMZ01000050 Genomic DNA Translation: KGM79834.1
JUDN01000073 Genomic DNA Translation: KHJ17794.1
JZDU01000179 Genomic DNA Translation: KJW22478.1
JZDZ01000075 Genomic DNA Translation: KJW50286.1
LEUM01000001 Genomic DNA Translation: KLY04786.1
LGZN01000008 Genomic DNA Translation: KNF71902.1
LGZP01000016 Genomic DNA Translation: KNF80630.1
LDYI01000114 Genomic DNA Translation: KPO09899.1
LIVE01000002 Genomic DNA Translation: KQJ19130.1
LIVI01000012 Genomic DNA Translation: KQJ42204.1
LMBK01000498 Genomic DNA Translation: KST28507.1
LNHR01000091 Genomic DNA Translation: KSX94159.1
LSNZ01000086 Genomic DNA Translation: KXG98721.1
LPXZ01000183 Genomic DNA Translation: KXL60979.1
LOOZ01000011 Genomic DNA Translation: KXR92089.1
LOFS01000184 Genomic DNA Translation: KYR47176.1
LOCS01000187 Genomic DNA Translation: KYV63191.1
LCVH01000002 Genomic DNA Translation: OAC45378.1
LZEH01000266 Genomic DNA Translation: OEI58961.1
MIWY01000006 Genomic DNA Translation: OEN36044.1
MOFD01000048 Genomic DNA Translation: OJK73558.1
MOGU01000021 Genomic DNA Translation: OJM94989.1
MOIL01000015 Genomic DNA Translation: OJP12822.1
MOJX01000010 Genomic DNA Translation: OJR05340.1
MOKZ01000004 Genomic DNA Translation: OJS90695.1
MPGT01000001 Genomic DNA Translation: OKB89803.1
MSEK01000030 Genomic DNA Translation: OLR88733.1
MTKH01000187 Genomic DNA Translation: ONG25915.1
MTPS01000255 Genomic DNA Translation: ONG33926.1
MCNL01000379 Genomic DNA Translation: ONK54063.1
NAFT01000003 Genomic DNA Translation: ORT10577.1
NEME01000004 Genomic DNA Translation: OTB40202.1
NBCM01000016 Genomic DNA Translation: OTE92569.1
MUAB01000024 Genomic DNA Translation: OUK50841.1
LYBG01000819 Genomic DNA Translation: OWD67099.1
NACG01000001 Genomic DNA Translation: OWX87637.1
LEOO01000028 Genomic DNA Translation: OYC64409.1
NQIT01000047 Genomic DNA Translation: OZX93798.1
MVPO01000026 Genomic DNA Translation: PAR03148.1
NWAO01000006 Genomic DNA Translation: PBR53162.1
NTEV01000146 Genomic DNA Translation: PBU42717.1
MOXU01000015 Genomic DNA Translation: PGG40139.1
PEDQ01000044 Genomic DNA Translation: PHW99629.1
JWSO02000012 Genomic DNA Translation: PNO97143.1
PPHQ01000007 Genomic DNA Translation: PNY67900.1
PSNR01000002 Genomic DNA Translation: PPE37663.1
PSSV01000004 Genomic DNA Translation: PPI96019.1
PTNY01000007 Genomic DNA Translation: PPZ57059.1
PUJR01000006 Genomic DNA Translation: PQP11279.1
PUQC01000195 Genomic DNA Translation: PQP27460.1
PVPD01000002 Genomic DNA Translation: PSG64000.1
NOLW03000053 Genomic DNA Translation: PSM33173.1
PYQK01000004 Genomic DNA Translation: PSY73785.1
PYQW01000003 Genomic DNA Translation: PSY81453.1
PYQU01000007 Genomic DNA Translation: PSY94222.1
PYQV01000003 Genomic DNA Translation: PSZ21693.1
PYRW01000008 Genomic DNA Translation: PSZ85513.1
RefSeqiWP_000130056.1, NZ_QBRW01000014.1

Genome annotation databases

EnsemblBacteriaiAIX61817; AIX61817; ECONIH1_00510
AJE54391; AJE54391; AC789_1c00920
ALL87292; ALL87292; MJ49_06580
ALY11550; ALY11550; ACN002_0092
ALZ67075; ALZ67075; AWH59_13345
ANM85311; ANM85311; A8V37_23525
ANO87245; ANO87245; GJ11_00490
ANP16212; ANP16212; GJ12_00490
AOM48083; AOM48083; FORC28_5107
AOM68394; AOM68394; MS6198_01060
CDU40703; CDU40703; PGD_03733
CTS13247; CTS13247; ERS085365_01030
CTT31890; CTT31890; ERS085379_00613
CTV24037; CTV24037; ERS139211_00749
CTV54797; CTV54797; ERS085416_01582
CTX15751; CTX15751; ERS139235_01279
CTY13197; CTY13197; ERS139266_02404
CTZ22945; CTZ22945; ERS150876_00142
KFD77273; KFD77273; JD73_13510
KGM67917; KGM67917; EL75_3670
KGM77191; KGM77191; EL80_3727
KGM79834; KGM79834; EL79_3780
KHH07020; KHH07020; PU69_20425
KHJ17794; KHJ17794; PU06_15025
KIG81022; KIG81022; PU38_12125
KIH05999; KIH05999; PU21_08070
KIH34994; KIH34994; PD07_16595
KIQ46899; KIQ46899; IY32_08180
KJW22478; KJW22478; UN86_27885
KJW50286; KJW50286; UN91_10970
KLX57401; KLX57401; SK78_02604
KLX88225; KLX88225; SK83_02692
KLY04786; KLY04786; SK85_00090
KME75430; KME75430; SM09_00836
KNF71902; KNF71902; WR15_01945
KNF80630; KNF80630; WQ89_09170
KNY77441; KNY77441; AGA26_05895
KPO09899; KPO09899; ACU57_16065
KPO35985; KPO35985; ACU81_18180
KQJ19130; KQJ19130; AM266_01485
KQJ42204; KQJ42204; AM270_12055
KST28507; KST28507; APZ14_15185
KSX94159; KSX94159; APT94_27415
KXG98721; KXG98721; HMPREF3040_02529
KXL60979; KXL60979; AUS26_14655
KXQ36579; KXQ36579; AUQ01_21005
KYR47176; KYR47176; AML07_23605
KYV63191; KYV63191; AMK83_01600
KZJ55379; KZJ55379; AWG90_06655
OAC45378; OAC45378; EC3234A_2c00760
OAJ86221; OAJ86221; A5958_05810
OAO70539; OAO70539; OO96_05110
OAS92324; OAS92324; A6I92_00685
OCO32447; OCO32447; AN669_0200600
ODA84481; ODA84481; A9D65_12690
ODH24296; ODH24296; A6804_06060
ODH28795; ODH28795; A6803_16340
OEI58961; OEI58961; A9R57_20470
OEN36044; OEN36044; BHF46_08275

Similar proteinsi

Entry informationi

Entry nameiJ7QWX0_ECOLX
AccessioniPrimary (citable) accession number: J7QWX0
Secondary accession number(s): A0A0J3ZAN0
Entry historyiIntegrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: October 31, 2012
Last modified: July 18, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)


Keywords - Technical termi

Complete proteomeImported

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health