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Entry version 46 (11 Dec 2019)
Sequence version 1 (05 Sep 2012)
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Protein

Assimilatory nitrate reductase

Gene

nasA

Organism
Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation. Catalyzes the reduction of nitrate to nitrite, using ferredoxin as the electron donor. Can use reduced methyl viologen but neither NADPH nor NADH as electron donors.1 Publication

Miscellaneous

Enzyme stability and activity depend upon the salt concentration.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by cyanide and azide.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.95 mM for nitrate1 Publication
  2. KM=0.066 mM for methyl viologen1 Publication

    pH dependencei

    Optimum pH is 9.1 Publication

    Temperature dependencei

    Optimum temperature is 80 degrees Celsius at 2.2 and 3.1 M NaCl, and 60 degrees Celsius at 0.9 and 1.3 M NaCl.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: nitrate reduction (assimilation)

    This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.1 Publication
    View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi22Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi25Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi29Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi59Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    Biological processNitrate assimilation
    Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    HMED523841:G1HBL-2728-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00653

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Assimilatory nitrate reductase2 Publications (EC:1.7.7.21 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:nasA1 Publication
    Ordered Locus Names:HFX_2002
    ORF Names:C439_11788
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHaloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri523841 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaStenosarchaea groupHalobacteriaHaloferacalesHaloferacaceaeHaloferax
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000011603 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence
    • UP000006469 Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004288921 – 708Assimilatory nitrate reductaseAdd BLAST708

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Repressed by the presence of ammonium as nitrogen source.1 Publication

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Is probably a monomer (PubMed:16182473). Initially characterized as a dimer of proteins with a MW of 105 and 50 kDa (PubMed:11731152).

    1 Publication1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    523841.HFX_2002

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    I3R634

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini15 – 734Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST59

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    KEGG Orthology (KO)

    More...
    KOi
    K00372

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    IISAPYE

    Database of Orthologous Groups

    More...
    OrthoDBi
    1029at2157

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR009010 Asp_de-COase-like_dom_sf
    IPR006657 MoPterin_dinucl-bd_dom
    IPR006656 Mopterin_OxRdtase
    IPR006963 Mopterin_OxRdtase_4Fe-4S_dom

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF04879 Molybdop_Fe4S4, 1 hit
    PF00384 Molybdopterin, 1 hit
    PF01568 Molydop_binding, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00926 Molybdop_Fe4S4, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF50692 SSF50692, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    I3R634-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPRNLRFLSV VNHVTKQVPT TCMRCAVGCG HVHLGSENAY GLETVRGDPS
    60 70 80 90 100
    HPVNNGLACG RGIRESADPA GEWLTRPLVR EDGELVQTSW SDAMARVGAT
    110 120 130 140 150
    IRTAVATDPD EVAVLGSGQQ TNEAAYALGK LARAGIGTRN YDANTTLCMA
    160 170 180 190 200
    SAVTAYYRAF GSDAPPPTYD DIPNAETHLV WGANPAVAHP VMFRWIRQSA
    210 220 230 240 250
    TDGRLVVVDP VETKTAAVAD DHVSVAPGGD LALARAILRH LVDTDQIDES
    260 270 280 290 300
    FVRSNTEGFD DVVSALPSVT DAAARAGVSL DTVEELAALL DAPTLIYWGM
    310 320 330 340 350
    GVNQSVRGTA TAGALVNLCL ASGNLGPGTG PFSLTGQANS MGTRVCSSKG
    360 370 380 390 400
    TWSGHRPFEH PDHRRAVAEA WDVPVSRLPD DSGPGPVGIL DSSPSVVWTV
    410 420 430 440 450
    ATNPLAGFPD ATAAREVLRD SFLVVQDAFR SDTVELADVV LPAATWGESE
    460 470 480 490 500
    GTAMNMERTV SRIRAATETP PGVRQDLDII ADVAARVAPG LLPRPPVSPS
    510 520 530 540 550
    AIFDEFAALT EGTDADCSGI SYTRLDGERA VRWPAPEPNS DAGYRYYDPS
    560 570 580 590 600
    TSRWTFPTPS GKARFSTLDG EPLPEPVDGD YPLTLTTGRE ADGYNTGVRS
    610 620 630 640 650
    RSDTPEEPVA RVNPETVDTY HDAVADTDGE LRTTVVSRRA SVSVTLDRDD
    660 670 680 690 700
    AVPPGLVWLS IHHPMTNQLT SPAVDPQSNE PNFKQCAVRF VHPDAPAKAD

    FLAAEVSD
    Length:708
    Mass (Da):75,211
    Last modified:September 5, 2012 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB8F054A1B6D7A525
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti200A → R in CAF19042 (PubMed:16182473).Curated1
    Sequence conflicti241L → I in CAF19042 (PubMed:16182473).Curated1
    Sequence conflicti347 – 352SSKGTW → PRKNV in CAF19042 (PubMed:16182473).Curated6
    Sequence conflicti445T → S in CAF19042 (PubMed:16182473).Curated1
    Sequence conflicti453A → P in CAF19042 (PubMed:16182473).Curated1
    Sequence conflicti538P → R in CAF19042 (PubMed:16182473).Curated1
    Sequence conflicti555T → R in CAF19042 (PubMed:16182473).Curated1
    Sequence conflicti612 – 617VNPETV → STPRPS in CAF19042 (PubMed:16182473).Curated6

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AJ621498 Genomic DNA Translation: CAF19042.1
    CP001868 Genomic DNA Translation: AFK19694.1
    AOLO01000009 Genomic DNA Translation: EMA00016.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_004059340.1, NZ_CP039139.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AFK19694; AFK19694; HFX_2002
    EMA00016; EMA00016; C439_11788

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    40155088

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    hme:HFX_2002

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|523841.21.peg.2383

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ621498 Genomic DNA Translation: CAF19042.1
    CP001868 Genomic DNA Translation: AFK19694.1
    AOLO01000009 Genomic DNA Translation: EMA00016.1
    RefSeqiWP_004059340.1, NZ_CP039139.1

    3D structure databases

    SMRiI3R634
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi523841.HFX_2002

    Genome annotation databases

    EnsemblBacteriaiAFK19694; AFK19694; HFX_2002
    EMA00016; EMA00016; C439_11788
    GeneIDi40155088
    KEGGihme:HFX_2002
    PATRICifig|523841.21.peg.2383

    Phylogenomic databases

    KOiK00372
    OMAiIISAPYE
    OrthoDBi1029at2157

    Enzyme and pathway databases

    UniPathwayiUPA00653
    BioCyciHMED523841:G1HBL-2728-MONOMER

    Family and domain databases

    InterProiView protein in InterPro
    IPR009010 Asp_de-COase-like_dom_sf
    IPR006657 MoPterin_dinucl-bd_dom
    IPR006656 Mopterin_OxRdtase
    IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
    PfamiView protein in Pfam
    PF04879 Molybdop_Fe4S4, 1 hit
    PF00384 Molybdopterin, 1 hit
    PF01568 Molydop_binding, 1 hit
    SMARTiView protein in SMART
    SM00926 Molybdop_Fe4S4, 1 hit
    SUPFAMiSSF50692 SSF50692, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNASA_HALMT
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: I3R634
    Secondary accession number(s): Q703N5
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: September 5, 2012
    Last modified: December 11, 2019
    This is version 46 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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