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Protein

Cyanide hydratase

Gene

cht

Organism
Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydration of cyanide to formamide. Degradation of cyanide may be important for plant pathogenic fungi in infection of cyanogenic plants.UniRule annotation1 Publication

Catalytic activityi

Formamide = cyanide + H2O.UniRule annotation1 Publication

pH dependencei

Optimum pH is 6-7. Active fom pH 6 to pH 8.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei46Proton acceptorPROSITE-ProRule annotation1
Active sitei128PROSITE-ProRule annotation1
Active sitei163NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase

Names & Taxonomyi

Protein namesi
Recommended name:
Cyanide hydratase1 PublicationUniRule annotation (EC:4.2.1.66UniRule annotation1 Publication)
Short name:
CHT1 PublicationUniRule annotation
Alternative name(s):
Cyanide-degrading nitrilaseUniRule annotation
Formamide hydrolyaseUniRule annotation
Gene namesi
Name:cht1 Publication
ORF Names:FGRAMPH1_01T18799, FGSG_05805
OrganismiGibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum)
Taxonomic identifieri229533 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusarium
Proteomesi
  • UP000070720 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiFungiDB:FGRAMPH1_01G18799

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004400331 – 355Cyanide hydrataseAdd BLAST355

Expressioni

Inductioni

By cyanide.UniRule annotation

Interactioni

Subunit structurei

Oligomer of dimers, forming left-handed helical fibers.UniRule annotation

Protein-protein interaction databases

STRINGi229533.XP_385981.1

Structurei

3D structure databases

ProteinModelPortaliI1RP53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 285CN hydrolasePROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0805 Eukaryota
COG0388 LUCA
InParanoidiI1RP53
KOiK10675
OrthoDBiEOG092C351H

Family and domain databases

Gene3Di3.60.110.10, 1 hit
HAMAPiMF_03224 CN_hydrolase, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR037544 CN_hydrolase
IPR000132 Nitrilase/CN_hydratase_CS
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS00920 NITRIL_CHT_1, 1 hit
PS00921 NITRIL_CHT_2, 1 hit

Sequencei

Sequence statusi: Complete.

I1RP53-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAITKYKAAA VTSEPGWFDL EGGVRKTIDF INEAGQAGCK FVAFPEVWIP
60 70 80 90 100
GYPYWMWKVT YLQSLPMLKR YRENSMAVDS EEMRRIRRAA RDNQIFVSLG
110 120 130 140 150
FSEIDHATLY LSQVLIGPDG AVINHRRKIK PTHVEKLVYG DGAGDTFMSV
160 170 180 190 200
SETEIGRVGQ LNCWENMNPF LKSLNVSAGE QVHVAAWPVY PGKERQVYPD
210 220 230 240 250
PATNYADPAS DLVTPEYAIE TGTWTLAPFQ RLSVEGLKIN TPEGVEPETD
260 270 280 290 300
PSVYNGHARI YRPDGSLVVK PEKDFDGLLF VDIDLNECHL TKVLADFAGH
310 320 330 340 350
YMRPDLIRLL VDTRRKELIT EADPNGSIAT YSTRQRLGLD KPLEKKEGED

TPDVL
Length:355
Mass (Da):39,927
Last modified:June 13, 2012 - v1
Checksum:iAD66DC0F9FC5DBB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HG970334 Genomic DNA Translation: CEF86019.1
DS231665 Genomic DNA Translation: ESU11821.1
RefSeqiXP_011324397.1, XM_011326095.1

Genome annotation databases

GeneIDi23552966
KEGGifgr:FGSG_05805

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HG970334 Genomic DNA Translation: CEF86019.1
DS231665 Genomic DNA Translation: ESU11821.1
RefSeqiXP_011324397.1, XM_011326095.1

3D structure databases

ProteinModelPortaliI1RP53
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi229533.XP_385981.1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23552966
KEGGifgr:FGSG_05805

Organism-specific databases

EuPathDBiFungiDB:FGRAMPH1_01G18799

Phylogenomic databases

eggNOGiKOG0805 Eukaryota
COG0388 LUCA
InParanoidiI1RP53
KOiK10675
OrthoDBiEOG092C351H

Family and domain databases

Gene3Di3.60.110.10, 1 hit
HAMAPiMF_03224 CN_hydrolase, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR037544 CN_hydrolase
IPR000132 Nitrilase/CN_hydratase_CS
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS00920 NITRIL_CHT_1, 1 hit
PS00921 NITRIL_CHT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHT_GIBZE
AccessioniPrimary (citable) accession number: I1RP53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
Last sequence update: June 13, 2012
Last modified: October 10, 2018
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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