UniProtKB - I1RN13 (SIDA_GIBZE)
L-ornithine N(5)-monooxygenase
SID1
Functioni
L-ornithine N5-monooxygenase; part of the gene cluster that mediates the biosynthesis of hydroxamate-containing siderophores that play a critical role in virulence (PubMed:20507510).
Gibberella zeae produces extracellular coprogen-type siderophores as well as the intracellular siderophore ferricrocin (PubMed:17056706).
The role of extracellular siderophores is to supply iron to the fungus during plant infection, and the intracellular ferricrocin is required for intracellular iron distribution and storage with a crucial role in ascus and ascospore development (PubMed:17056706, PubMed:17043871, PubMed:17601875).
SID1 catalyzes the conversion of L-ornithine to N5-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores (PubMed:20507510).
The assembly of extracellular coprogen-type siderophores is performed by the nonribosomal peptide synthetase (NRPS) NPS6 whereas the intracellular siderophore ferricrocin is assembled by NPS2 (PubMed:17056706, PubMed:17043871, PubMed:17601875).
4 PublicationsCatalytic activityi
- EC:1.14.13.196By similarity
- EC:1.14.13.196By similarity
Cofactori
: Siderophore biosynthesis Pathwayi
This protein is involved in Siderophore biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 135 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 140 | SubstrateBy similarity | 1 | |
Binding sitei | 201 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 355 | SubstrateBy similarity | 1 | |
Binding sitei | 520 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 116 – 124 | FADBy similarity | 9 | |
Nucleotide bindingi | 286 – 289 | NADPBy similarity | 4 | |
Nucleotide bindingi | 355 – 357 | NADPBy similarity | 3 | |
Nucleotide bindingi | 517 – 519 | FADBy similarity | 3 |
GO - Molecular functioni
- ornithine N5-monooxygenase activity Source: RHEA
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: L-ornithine N(5)-monooxygenase1 Publication (EC:1.14.13.1961 Publication)Short name: OMOBy similarity Alternative name(s): L-ornithine N(5)-oxygenase1 Publication Siderophore biosynthesis protein A1 Publication |
Gene namesi | Name:SID11 Publication ORF Names:FG05371, FGRAMPH1_01T17749 |
Organismi | Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) |
Taxonomic identifieri | 229533 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Fusarium › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:FGRAMPH1_01G17749 |
Pathology & Biotechi
Disruption phenotypei
Miscellaneous databases
PHI-basei | PHI:1010 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000444438 | 1 – 536 | L-ornithine N(5)-monooxygenaseAdd BLAST | 536 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 5518.FGSG_05371P0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 64 | DisorderedSequence analysisAdd BLAST | 64 | |
Regioni | 325 – 328 | Substrate bindingBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 47 | Polar residuesSequence analysisAdd BLAST | 47 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1399, Eukaryota |
HOGENOMi | CLU_020931_2_0_1 |
InParanoidi | I1RN13 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR025700, Lys/Orn_oxygenase |
PANTHERi | PTHR42802, PTHR42802, 2 hits |
Pfami | View protein in Pfam PF13434, K_oxygenase, 1 hit |
SUPFAMi | SSF51905, SSF51905, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSPHSEISFS GSDAAVSNGT SNGINGHSNG TNGFSNGHMN GHSNGNDAPT
60 70 80 90 100
AVRHGPRVHK ERSRHLETAP VDSEFDLICA GFGPASLAVA VAMHDAIAEG
110 120 130 140 150
RNLRPDGTAP KVLFLEKQPK FAWHAGMLLP GAKMQISFIK DMATLRNPRS
160 170 180 190 200
EFTFLNYLHS QGRLVDFTNL GTFLPARTEY EDYLRWCSSW FDHVVNYNNE
210 220 230 240 250
VLSISPENKE AGAVKTFTVQ ARNGKTGQIQ SFRSRHVLVA AGGQPSLPKS
260 270 280 290 300
LPAKHPRVLH SSQFANYAPQ ILTKQNAPYR VAVIGAGQSA AEIFNNVQNL
310 320 330 340 350
YPNSKTYLIM RQEFLRPSDD SPFVNSIFNP EYIDNLWPRS VKARETLLTE
360 370 380 390 400
ARATNYGVVR LELIEHLFEK MYDQKREISD DETQWPHRIL SGREIASVDT
410 420 430 440 450
KGDALEIKVQ RVNDGPLDGF VDQETFDVDL IIAATGYKRS AHIDMLKDAW
460 470 480 490 500
TMLPKTVSGR NESPKGANGW NVETQDGERK LAVARDYRVK FSPGTVADDS
510 520 530
GVWLQGCCEG THGLSDTLLS VLSTRSGEIV QSIFKQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HG970334 Genomic DNA Translation: CEF88195.1 |
RefSeqi | XP_011323895.1, XM_011325593.1 |
Genome annotation databases
GeneIDi | 23552556 |
KEGGi | fgr:FGSG_05371 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HG970334 Genomic DNA Translation: CEF88195.1 |
RefSeqi | XP_011323895.1, XM_011325593.1 |
3D structure databases
AlphaFoldDBi | I1RN13 |
SMRi | I1RN13 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 5518.FGSG_05371P0 |
Genome annotation databases
GeneIDi | 23552556 |
KEGGi | fgr:FGSG_05371 |
Organism-specific databases
VEuPathDBi | FungiDB:FGRAMPH1_01G17749 |
Phylogenomic databases
eggNOGi | KOG1399, Eukaryota |
HOGENOMi | CLU_020931_2_0_1 |
InParanoidi | I1RN13 |
Miscellaneous databases
PHI-basei | PHI:1010 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR025700, Lys/Orn_oxygenase |
PANTHERi | PTHR42802, PTHR42802, 2 hits |
Pfami | View protein in Pfam PF13434, K_oxygenase, 1 hit |
SUPFAMi | SSF51905, SSF51905, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SIDA_GIBZE | |
Accessioni | I1RN13Primary (citable) accession number: I1RN13 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2018 |
Last sequence update: | June 13, 2012 | |
Last modified: | May 25, 2022 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families