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Protein

Bifunctional cis-abienol synthase, chloroplastic

Gene

CAS

Organism
Abies balsamea (Balsam fir) (Pinus balsamea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of cis-abienol, a labdane diterpene that can be used as synthesis precursor of ambergris substitution fragance products. Bifunctional class I/II enzyme in which both the bicyclization and water capture occur in the class II active site, resulting in an intermediary labda-13-en-8-ol diphosphate, which undergoes cleavage of the diphosphate group and final deprotonation at the class I active site. No activity with copalyl diphosphate as substrate.1 Publication

Catalytic activityi

(13E)-8-alpha-hydroxylabd-13-en-15-yl diphosphate = cis-abienol + diphosphate.1 Publication
(13E)-8-alpha-hydroxylabd-13-en-15-yl diphosphate = geranylgeranyl diphosphate + H2O.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: oleoresin biosynthesis

This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi622Magnesium 1By similarity1
Metal bindingi622Magnesium 2By similarity1
Metal bindingi626Magnesium 1By similarity1
Metal bindingi626Magnesium 2By similarity1
Metal bindingi763Magnesium 3By similarity1
Metal bindingi767Magnesium 3By similarity1
Metal bindingi771Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Multifunctional enzyme
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.3.140 13176
UniPathwayiUPA00924

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional cis-abienol synthase, chloroplastic (EC:4.2.1.133, EC:4.2.3.140)
Alternative name(s):
Diterpene synthase TPS4
Short name:
AbdiTPS4
Gene namesi
Name:CAS
OrganismiAbies balsamea (Balsam fir) (Pinus balsamea)
Taxonomic identifieri90345 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi403 – 405DID → AIA: Loss of activity. 1 Publication3
Mutagenesisi622D → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49ChloroplastSequence analysisAdd BLAST49
ChainiPRO_000042333850 – 867Bifunctional cis-abienol synthase, chloroplasticAdd BLAST818

Structurei

3D structure databases

ProteinModelPortaliH8ZM73
SMRiH8ZM73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi403 – 406DXDD motifCurated4
Motifi622 – 626DDXXD motifCurated5

Domaini

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.Curated

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsd subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK20518

Family and domain databases

Gene3Di1.10.600.10, 1 hit
1.50.10.130, 2 hits
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR034741 Terpene_cyclase_like_1_C
IPR001906 Terpene_synth_N
IPR036965 Terpene_synth_N_sf
IPR005630 Terpene_synthase_metal-bd
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF01397 Terpene_synth, 1 hit
PF03936 Terpene_synth_C, 1 hit
SFLDiSFLDG01019 Terpene_Cyclase_Like_1_C_Termi, 1 hit
SUPFAMiSSF48239 SSF48239, 3 hits
SSF48576 SSF48576, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

H8ZM73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPVYSLKS HIPITTIASA KMNYTPNKGM ITANGRSRRI RLSPNKIVAC
60 70 80 90 100
AGEADRTFPS QSLEKTALFP DQFSEKNGTP SNFTPPNREF PPSFWNNDII
110 120 130 140 150
NSITASHKVQ TGDRKRIQTL ISEIKNVFNS MGDGETSPSA YDTAWVARIP
160 170 180 190 200
AVDGSEQPQF PQTLEWILQN QLKDGSWGEE FYFLAYDRLL ATLACIITLT
210 220 230 240 250
IWRTGNVQLH KGIEFFRKQV VRMDDEADNH RPSGFEIVFP AMLNEAKSLG
260 270 280 290 300
LDLPYELPFI EQMVKKREAK LKMITTNVLY TIQTTLLYSL EGLHEIVDFD
310 320 330 340 350
KIIKLQSKDG SFLGSPASTA AVFMQTGNTK CLEFLEFVLR KFRNHVPSDY
360 370 380 390 400
PLDLFERLWV VDTVERLGID RHFKKEIKDA LDYVYSCWDE RGIGWAKDSP
410 420 430 440 450
IADIDDTAMG LRILRLHGYN VSPDVLKTFK DENGEFFCFM GQTQRGVTDM
460 470 480 490 500
LNVYRCSQVA FPGETIMEEA KLCTERYLRN ALENADAFDK WAIKKNIRGE
510 520 530 540 550
VEYALKYPWH RSMPRLEVRS YIGNYGPNDV WLGKSLYMMP YISNEKYLEL
560 570 580 590 600
AKLDFNSVQS LHQEEIRELV RWCKSSGFTE LKFTRDRVVE TYFAVASSMF
610 620 630 640 650
EPEFSTCRAV YTKISVLLVI LDDLYDGYGS PDEIKLFSEA VKRWDLSLLE
660 670 680 690 700
QMPDHMKICF LGLYNTVNEV AEEGRKTQGH DVLGYIRNLW EIQLAAFTRE
710 720 730 740 750
AEWSQGKYVP SFDEYIENAQ VSIGVATILL ITILFTEEDD ILSHIDYGSK
760 770 780 790 800
FLRLASLTAR LANDIKTYQE ERAHGEVVSA IQCYMKDRPE ITEEEALKYV
810 820 830 840 850
YGRMVNDLAE LNSEYLKSNE MPQNCKRLVF DTARVAQLFT MEGDGLTYSD
860
TMEIKEHIKK CLFEPAT
Length:867
Mass (Da):99,539
Last modified:May 16, 2012 - v1
Checksum:i7C9A13BFDA8134C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JN254808 mRNA Translation: AEL99953.1

Genome annotation databases

KEGGiag:AEL99953

Similar proteinsi

Entry informationi

Entry nameiCAS_ABIBA
AccessioniPrimary (citable) accession number: H8ZM73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: May 16, 2012
Last modified: February 28, 2018
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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