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UniProtKB - H8ZM70 (LAS_ABIBA)
Protein
Bifunctional abietadiene synthase, chloroplastic
Gene
LAS
Organism
Abies balsamea (Balsam fir) (Pinus balsamea)
Status
Functioni
Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to abietadiene. The copalyl diphosphate (CPP) intermediate diffuses freely between the 2 active sites in the enzyme.
1 PublicationCatalytic activityi
- EC:5.5.1.121 Publication
- EC:4.2.3.181 Publication
- EC:4.2.3.1321 Publication
- EC:4.2.3.321 Publication
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
: oleoresin biosynthesis Pathwayi
This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.View all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 245 | SubstrateBy similarity | 1 | |
Metal bindingi | 378 | Magnesium 4By similarity | 1 | |
Metal bindingi | 380 | Magnesium 4By similarity | 1 | |
Binding sitei | 465 | SubstrateBy similarity | 1 | |
Metal bindingi | 597 | Magnesium 1By similarity | 1 | |
Metal bindingi | 597 | Magnesium 2By similarity | 1 | |
Metal bindingi | 601 | Magnesium 1By similarity | 1 | |
Metal bindingi | 601 | Magnesium 2By similarity | 1 | |
Metal bindingi | 741 | Magnesium 3By similarity | 1 | |
Metal bindingi | 745 | Magnesium 3By similarity | 1 | |
Metal bindingi | 749 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- abietadiene synthase activity Source: UniProtKB-EC
- copalyl diphosphate synthase activity Source: UniProtKB-EC
- levopimaradiene synthase activity Source: UniProtKB-EC
- magnesium ion binding Source: InterPro
- terpene synthase activity Source: InterPro
GO - Biological processi
- diterpenoid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00924 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional abietadiene synthase, chloroplasticAlternative name(s): Abietadiene cyclase Diterpene synthase TPS1 Short name: AbdiTPS1 Levopimaradiene/abietadiene synthase Short name: AbLAS Including the following 2 domains: |
Gene namesi | Name:LAS |
Organismi | Abies balsamea (Balsam fir) (Pinus balsamea) |
Taxonomic identifieri | 90345 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Pinopsida › Pinidae › Conifers I › Pinaceae › Abies |
Subcellular locationi
Chloroplast
- chloroplast By similarity
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | ‹1 – 46 | ChloroplastSequence analysisAdd BLAST | ›46 | |
ChainiPRO_0000423339 | 47 – 844 | Bifunctional abietadiene synthase, chloroplasticAdd BLAST | 798 |
Interactioni
Subunit structurei
Monomer.
By similarityFamily & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 378 – 381 | DXDD motifCurated | 4 | |
Motifi | 597 – 601 | DDXXD motifCurated | 5 |
Domaini
The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.Curated
Sequence similaritiesi
Keywords - Domaini
Transit peptideFamily and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Fragment.
: The displayed sequence is further processed into a mature form. Sequence processingi
H8ZM70-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
QSIPHFSTTL NAGSSARKRR SLYLRWGKGS NKIIACVGEG ATSVPYQSAE
60 70 80 90 100
KNDSLYSSTL VKREFPPGFW KDDLIDSLTS SHKVAASDEK RIETLISEIK
110 120 130 140 150
NMFRCMGYGE TNPSAYDTAW VARIPALDGS DNPHFPETVE WILQNQLKDG
160 170 180 190 200
SWGEGFYFLA YDRILATLAC IITLTLWRTG ETQVHKGIEF FRTQAGKMED
210 220 230 240 250
EADSHRPSGF EIVFPAMLKE AKILGLDLPY DLPFLKQIIE KREAKLKRIP
260 270 280 290 300
TDVLYALPTT LLYSLEGLQE IVDWQKIMKL QSKDGSFLSS PASTAAVFMR
310 320 330 340 350
TGNKKCLDFL NFVLKKFGNH VPCHYPLDLF ERLWAVDTVE RLGIDRHFKE
360 370 380 390 400
EIKEALDYVY SHWDERGIGW ARENPVPDID DTAMGLRILR LHGYNVSSDV
410 420 430 440 450
LKTFRDENGE FFCFLGQTQR GVTDMLNVNR CSHVSFPGET IMEEAKLCTE
460 470 480 490 500
RYLRNALENV DAFDKWAFKK NIRGEVEYAL KYPWHKSMPR LEARSYIENY
510 520 530 540 550
GPDDVWLGKT VYMMPYISNE KYLELAKLDF NKVQSIHQTE LQDLRRWWKS
560 570 580 590 600
SGFTDLNFTR ERVTEIYFSP ASFIFEPEFS KCREVYTKTS NFTVILDDLY
610 620 630 640 650
DAHGSLDDLK LFTESVKRWD LSLVDQMPQQ MKICFVGFYN TFNEIAKEGR
660 670 680 690 700
ESQGRDVLGY IQNVWKVQLE AYTKEAEWSE AKYVPSFNEY IENASVSIAL
710 720 730 740 750
GTVVLISALF TGEVLTDEVL SKIDRGSRFL QLMGLTGRLV NDTKTYQAER
760 770 780 790 800
GQGEVASAIQ CYMKDHPKIS EEEALKHVYT VMENSLEELN REFVNNKIPD
810 820 830 840
IYRRLVFETA RIMQLFYMQG DGLTLSHDME IKEHVKNCLF QPVA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JN254805 mRNA Translation: AEL99950.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JN254805 mRNA Translation: AEL99950.1 |
3D structure databases
SMRi | H8ZM70 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00924 |
Family and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LAS_ABIBA | |
Accessioni | H8ZM70Primary (citable) accession number: H8ZM70 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 18, 2013 |
Last sequence update: | May 16, 2012 | |
Last modified: | September 29, 2021 | |
This is version 32 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families