UniProtKB - H8EVV4 (ACEA1_MYCTE)
Protein
Isocitrate lyase 1
Gene
icl1
Organism
Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)
Status
Functioni
Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It also catalyzes the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).1 Publication
Miscellaneous
Cell growing on 0.2% glucose show barely detectable isocitrate and methylisocitrate lyase activities. On 0.1% and 0.2% propionate, the lyase activities increase more than 10 and 100-fold, respectively.1 Publication
Catalytic activityi
Cofactori
Mg2+By similarity
: glyoxylate cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Isocitrate lyase 1 (icl1), Isocitrate lyase 2 (aceAb)
- Malate synthase G (glcB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 153 | MagnesiumBy similarity | 1 | |
Active sitei | 191 | Proton acceptorBy similarity | 1 | |
Binding sitei | 228 | SubstrateBy similarity | 1 | |
Binding sitei | 347 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- isocitrate lyase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- methylisocitrate lyase activity Source: UniProtKB-EC
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | MTUB652616:G1HGE-499-MONOMER |
UniPathwayi | UPA00703;UER00719 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:icl1 Ordered Locus Names:ERDMAN_0512 ORF Names:Q643_00485 |
Organismi | Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman) |
Taxonomic identifieri | 652616 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion of icl1 in cells growing on 0.1% propionate shows 10 and 8-fold decrease of isocitrate and methylisocitrate lyase activity, respectively.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000432564 | 1 – 428 | Isocitrate lyase 1Add BLAST | 428 |
Interactioni
Subunit structurei
Homotetramer.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 93 | Substrate bindingBy similarity | 3 | |
Regioni | 192 – 193 | Substrate bindingBy similarity | 2 | |
Regioni | 313 – 317 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_019214_2_0_11 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 2 hits |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 2 hits |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
i Sequence
Sequence statusi: Complete.
H8EVV4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVVGTPKSA EQIQQEWDTN PRWKDVTRTY SAEDVVALQG SVVEEHTLAR
60 70 80 90 100
RGAEVLWEQL HDLEWVNALG ALTGNMAVQQ VRAGLKAIYL SGWQVAGDAN
110 120 130 140 150
LSGHTYPDQS LYPANSVPQV VRRINNALQR ADQIAKIEGD TSVENWLAPI
160 170 180 190 200
VADGEAGFGG ALNVYELQKA LIAAGVAGSH WEDQLASEKK CGHLGGKVLI
210 220 230 240 250
PTQQHIRTLT SARLAADVAD VPTVVIARTD AEAATLITSD VDERDQPFIT
260 270 280 290 300
GERTREGFYR TKNGIEPCIA RAKAYAPFAD LIWMETGTPD LEAARQFSEA
310 320 330 340 350
VKAEYPDQML AYNCSPSFNW KKHLDDATIA KFQKELAAMG FKFQFITLAG
360 370 380 390 400
FHALNYSMFD LAYGYAQNQM SAYVELQERE FAAEERGYTA TKHQREVGAG
410 420
YFDRIATTVD PNSSTTALTG STEEGQFH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP012340 Genomic DNA Translation: BAL64328.1 JLBG01000002 Genomic DNA Translation: KBK18542.1 |
RefSeqi | WP_003402316.1, NZ_KK339487.1 |
Genome annotation databases
EnsemblBacteriai | BAL64328; BAL64328; ERDMAN_0512 KBK18542; KBK18542; Q643_00485 |
KEGGi | mtn:ERDMAN_0512 |
PATRICi | fig|652616.3.peg.519 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP012340 Genomic DNA Translation: BAL64328.1 JLBG01000002 Genomic DNA Translation: KBK18542.1 |
RefSeqi | WP_003402316.1, NZ_KK339487.1 |
3D structure databases
SMRi | H8EVV4 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | BAL64328; BAL64328; ERDMAN_0512 KBK18542; KBK18542; Q643_00485 |
KEGGi | mtn:ERDMAN_0512 |
PATRICi | fig|652616.3.peg.519 |
Phylogenomic databases
HOGENOMi | CLU_019214_2_0_11 |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
BioCyci | MTUB652616:G1HGE-499-MONOMER |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 2 hits |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 2 hits |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACEA1_MYCTE | |
Accessioni | H8EVV4Primary (citable) accession number: H8EVV4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 2015 |
Last sequence update: | May 16, 2012 | |
Last modified: | February 10, 2021 | |
This is version 48 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families