UniProtKB - H2T155 (H2T155_TAKRU)
Protein
NADPH--cytochrome P450 reductase
Gene
por
Organism
Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)
Status
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation
Caution
Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
Catalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 204 | FMNUniRule annotation | 1 | |
Binding sitei | 294 | NADPUniRule annotation | 1 | |
Binding sitei | 474 | FADUniRule annotation | 1 | |
Binding sitei | 530 | NADPUniRule annotation | 1 | |
Binding sitei | 634 | NADPUniRule annotation | 1 | |
Binding sitei | 672 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 81 – 86 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 133 – 136 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 169 – 178 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 450 – 453 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 468 – 470 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 484 – 487 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 591 – 592 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 597 – 601 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: UniProtKB-UniRule
- FMN binding Source: UniProtKB-UniRule
- NADP binding Source: UniProtKB-UniRule
- NADPH-hemoprotein reductase activity Source: UniProtKB-UniRule
Keywordsi
Molecular function | OxidoreductaseUniRule annotation |
Ligand | FADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)Short name: CPRUniRule annotation Short name: P450RUniRule annotation |
Gene namesi | Name:porImported Synonyms:PORUniRule annotation |
Organismi | Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)Imported |
Taxonomic identifieri | 31033 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Neoteleostei › Acanthomorphata › Eupercaria › Tetraodontiformes › Tetradontoidea › Tetraodontidae › Takifugu |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 21 – 39 | HelicalUniRule annotationAdd BLAST | 19 |
Keywords - Cellular componenti
Endoplasmic reticulumUniRule annotation, MembraneFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 75 – 220 | Flavodoxin-likeInterPro annotationAdd BLAST | 146 | |
Domaini | 275 – 516 | FAD-binding FR-typeInterPro annotationAdd BLAST | 242 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 574 – 594 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
Keywords - Domaini
Coiled coilSequence analysis, Transmembrane, Transmembrane helixUniRule annotationPhylogenomic databases
eggNOGi | KOG1158 Eukaryota COG0369 LUCA |
GeneTreei | ENSGT00940000156847 |
KOi | K00327 |
OrthoDBi | 318396at2759 |
TreeFami | TF105719 |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212 NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208 P450R, 1 hit |
PRINTSi | PR00369 FLAVODOXIN PR00371 FPNCR |
SUPFAMi | SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
H2T155-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRPPLRAHP WEEVEPLFSN LDIFLFSLIV GIVIYWFMFR KKPEPVPEFK
60 70 80 90 100
KIETQAPSTR ETSFIEKMKK MGKNIIVFYG SQTGTGEEFA NRLSKDAQRY
110 120 130 140 150
GMKGMAADPE EYDMAELSRL SEIENSLAIF CMATYGEGDP TDNAQDFYDW
160 170 180 190 200
LQENDDEELS GLNYTVFALG NKTYEHYNAM GKYVDKRLEE LGAKRIFDLG
210 220 230 240 250
LGDDDGNLEE DFVSWREQFW PAVCEHFGVE PLGDESSIRQ YELKVHTDIN
260 270 280 290 300
MNKVYSGEIG RLKSFEVQKP PFDSKNPFLA PVTVNRRLNK GGERHLMHLE
310 320 330 340 350
LDITGSKIRY ESGDHVAVFP TNDSALVNKL GQVLGVDLDV VISLNNLDEE
360 370 380 390 400
SNKKHPFPCP TTYRTALTHY LDITQPPRTN VLYELAQYAT DGKDQENLRK
410 420 430 440 450
MASSSPEGKS LYQNWVLDAC RNILAILEDM PSLRPPIDHL CELLPRLQAR
460 470 480 490 500
YYSIASSSKV HPNSIHICAV VVEYQTKTGR LNKGVATNWL KNKLISDNGH
510 520 530 540 550
KSMVPMYIRK SQFRLPFKAT NPVLMVGPGT GIAPFVGFIQ ERGWLKEQGK
560 570 580 590 600
EVGETVMYFG CRHKNEDYLY QEELEEAEKN AALTQLNVAF SRDQEQKVYV
610 620 630 640 650
QHLLKANKEH VWKLIHSENA HIYICGDARN MAKDVQLAFY EIAEEQGEMT
660 670
RSQATDYIKK LMTKGRYSQD VWS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH2T156 | H2T156_TAKRU | NADPH--cytochrome P450 reductase | por POR | 635 | Annotation score: |
Sequence databases
RefSeqi | XP_003968271.1, XM_003968222.2 XP_011606506.1, XM_011608204.1 XP_011606507.1, XM_011608205.1 |
Genome annotation databases
Ensembli | ENSTRUT00000018468; ENSTRUP00000018392; ENSTRUG00000007436 ENSTRUT00000018469; ENSTRUP00000018393; ENSTRUG00000007436 |
GeneIDi | 101077941 |
KEGGi | tru:101077941 |
Similar proteinsi
Cross-referencesi
Sequence databases
RefSeqi | XP_003968271.1, XM_003968222.2 XP_011606506.1, XM_011608204.1 XP_011606507.1, XM_011608205.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 31033.ENSTRUP00000018392 |
Genome annotation databases
Ensembli | ENSTRUT00000018468; ENSTRUP00000018392; ENSTRUG00000007436 ENSTRUT00000018469; ENSTRUP00000018393; ENSTRUG00000007436 |
GeneIDi | 101077941 |
KEGGi | tru:101077941 |
Phylogenomic databases
eggNOGi | KOG1158 Eukaryota COG0369 LUCA |
GeneTreei | ENSGT00940000156847 |
KOi | K00327 |
OrthoDBi | 318396at2759 |
TreeFami | TF105719 |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212 NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208 P450R, 1 hit |
PRINTSi | PR00369 FLAVODOXIN PR00371 FPNCR |
SUPFAMi | SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | H2T155_TAKRU | |
Accessioni | H2T155Primary (citable) accession number: H2T155 Secondary accession number(s): H2T154 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | March 21, 2012 |
Last sequence update: | March 21, 2012 | |
Last modified: | November 13, 2019 | |
This is version 55 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |