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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Methanosaeta harundinacea (strain 6Ac)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35SubstrateUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Active sitei98Proton donorUniRule annotation1
Sitei182Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei213SubstrateUniRule annotation1
Sitei229Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei239Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotation
Biological processAmino-acid biosynthesis, Lysine biosynthesisUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:Mhar_1870Imported
OrganismiMethanosaeta harundinacea (strain 6Ac)Imported
Taxonomic identifieri1110509 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanothrix
Proteomesi
  • UP000005877 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi1110509.Mhar_1870

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 100Substrate bindingUniRule annotation2
Regioni229 – 230Substrate bindingUniRule annotation2
Regioni240 – 241Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02255 Archaea
COG0253 LUCA
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG093Z085A

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit

Sequencei

Sequence statusi: Complete.

G7WQ35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYKMKLKEM TDARVEGSPT SSGRIAFTKL QGNGNDFVLI DEQDGVKVAD
60 70 80 90 100
PLKGSFAVQC CHRNFGVGGD GVLFLGASEH ADLRMRLFQP DGSEAEMCGN
110 120 130 140 150
GVRCLAKYAW DSGMVGESFE VETLAGIIPL MVRGIGGDFW ARVDMGVPCF
160 170 180 190 200
DRRGIPALGE GEFLMEPLEG FEVSAVNTGV PHAVIFVEDL LNLPLEEVAP
210 220 230 240 250
HIRRSSLFPE GANVNFVRIG DAFQVRTFER GVEGETLSCG TGSVASAAVA
260 270 280 290 300
RRMGMVGDEV FVETRGGPLL ITFDGDRAYM DGPAATVFAG ELSDEFHWDI

SQEL
Length:304
Mass (Da):32,798
Last modified:January 25, 2012 - v1
Checksum:i8B73F3C51DAB6021
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003117 Genomic DNA Translation: AET65227.1

Genome annotation databases

EnsemblBacteriaiAET65227; AET65227; Mhar_1870
KEGGimhi:Mhar_1870
PATRICifig|1110509.7.peg.2074

Similar proteinsi

Entry informationi

Entry nameiG7WQ35_METH6
AccessioniPrimary (citable) accession number: G7WQ35
Entry historyiIntegrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: March 28, 2018
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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