UniProtKB - G5EGH6 (FAT7_CAEEL)
Delta(9)-fatty-acid desaturase fat-7
fat-7
Functioni
Delta9-fatty acid desaturase that acts preferentially on stearoyl-CoA (octadecanoyl-CoA) producing the monounsaturated oleoyl-CoA ((9Z)-octadecenoyl-CoA), one of the most abundant monounsaturated fatty acid in Caenorhabditis elegans phospholipids and triacylglycerols. Also acts on palmitoyl-CoA (hexadecanoyl-CoA), heptadecanoyl-CoA and (11E)-octadecenoyl-CoA (trans-vaccenoyl-CoA), the monounsaturated fatty acids (MUFAs) produced are further used by several other desaturases and elongases as substrates to synthesize polyunsaturated fatty acids (PUFAs) endogenously (PUFAs are essential for membrane structure and many cellular and physiological processes) (PubMed:16839188, PubMed:10872837, PubMed:29237573).
Unlike plants, Caenorhabditis elegans desaturases seem to use fatty acyl-CoAs as substrates (By similarity).
By similarity3 PublicationsMiscellaneous
Catalytic activityi
- 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 + octadecanoyl-CoA = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O3 PublicationsEC:1.14.19.13 PublicationsThis reaction proceeds in the forward3 Publications direction.
- 2 Fe(II)-[cytochrome b5] + 2 H+ + hexadecanoyl-CoA + O2 = (9Z)-hexadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O2 Publications
- 2 Fe(II)-[cytochrome b5] + 2 H+ + heptadecanoyl-CoA + O2 = (9Z)-heptadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (11E)-octadecenoyl-CoA + 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 = (9Z,11E)-octadecadienoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O1 PublicationThis reaction proceeds in the forward1 Publication direction.
: monounsaturated fatty acid biosynthesis Pathwayi
This protein is involved in the pathway monounsaturated fatty acid biosynthesis, which is part of Lipid metabolism.2 PublicationsView all proteins of this organism that are known to be involved in the pathway monounsaturated fatty acid biosynthesis and in Lipid metabolism.
Pathwayi: fatty acid metabolism
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.2 PublicationsView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
GO - Molecular functioni
- iron ion binding Source: GO_Central
- stearoyl-CoA 9-desaturase activity Source: WormBase
GO - Biological processi
- fatty acid biosynthetic process Source: WormBase
- oxygen homeostasis Source: UniProtKB
- post-embryonic development Source: WormBase
- response to hypoxia Source: UniProtKB
- unsaturated fatty acid biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-CEL-75105, Fatty acyl-CoA biosynthesis |
UniPathwayi | UPA00199 UPA01038 |
Chemistry databases
SwissLipidsi | SLP:000000271 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fat-7 ORF Names:F10D2.9 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | F10D2.9 ; CE09320 ; WBGene00001399 ; fat-7 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 51 – 71 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 194 – 214 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 218 – 238 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423389 | 1 – 338 | Delta(9)-fatty-acid desaturase fat-7Add BLAST | 338 |
Proteomic databases
EPDi | G5EGH6 |
PaxDbi | G5EGH6 |
PeptideAtlasi | G5EGH6 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | WBGene00001399, Expressed in adult organism and 4 other tissues |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1600, Eukaryota |
GeneTreei | ENSGT00970000196153 |
HOGENOMi | CLU_027359_0_0_1 |
InParanoidi | G5EGH6 |
OMAi | CQHGPID |
OrthoDBi | 971318at2759 |
PhylomeDBi | G5EGH6 |
Family and domain databases
CDDi | cd03505, Delta9-FADS-like, 1 hit |
InterProi | View protein in InterPro IPR015876, Acyl-CoA_DS |
PANTHERi | PTHR11351, PTHR11351, 1 hit |
PRINTSi | PR00075, FACDDSATRASE |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTVKTRASIA KKIEKDGLDS QYLFMDPNEV LQVQEESKKI PYKMEIVWRN
60 70 80 90 100
VALFAALHVA AAIGLYELVF HAKWQTAVFS FALYVFSGFG ITAGAHRLWS
110 120 130 140 150
HKSYKATTPM RIFLMLLNNI ALQNDIIEWA RDHRCHHKWT DTDADPHNTT
160 170 180 190 200
RGFFFTHMGW LLVRKHPQVK EHGGKLDLSD LFSDPVLVFQ RKHYFPLVIL
210 220 230 240 250
FCFILPTIIP VYFWKETAFI AFYVAGTFRY CFTLHATWCI NSAAHYFGWK
260 270 280 290 300
PYDTSVSAVE NVFTTVVAVG EGGHNFHHTF PQDYRASEYS LIYNWTRVLI
310 320 330
DTAAVLGLVY DRKTIADEFI SRQVANHGSE ESRKKSIM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF260243 mRNA Translation: AAF97549.1 FO081104 Genomic DNA Translation: CCD69123.1 |
PIRi | T28723 |
RefSeqi | NP_504814.1, NM_072413.6 |
Genome annotation databases
EnsemblMetazoai | F10D2.9.1; F10D2.9.1; WBGene00001399 |
GeneIDi | 179100 |
KEGGi | cel:CELE_F10D2.9 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF260243 mRNA Translation: AAF97549.1 FO081104 Genomic DNA Translation: CCD69123.1 |
PIRi | T28723 |
RefSeqi | NP_504814.1, NM_072413.6 |
3D structure databases
SMRi | G5EGH6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 44145, 2 interactors |
STRINGi | 6239.F10D2.9 |
Chemistry databases
SwissLipidsi | SLP:000000271 |
Proteomic databases
EPDi | G5EGH6 |
PaxDbi | G5EGH6 |
PeptideAtlasi | G5EGH6 |
Genome annotation databases
EnsemblMetazoai | F10D2.9.1; F10D2.9.1; WBGene00001399 |
GeneIDi | 179100 |
KEGGi | cel:CELE_F10D2.9 |
Organism-specific databases
CTDi | 179100 |
WormBasei | F10D2.9 ; CE09320 ; WBGene00001399 ; fat-7 |
Phylogenomic databases
eggNOGi | KOG1600, Eukaryota |
GeneTreei | ENSGT00970000196153 |
HOGENOMi | CLU_027359_0_0_1 |
InParanoidi | G5EGH6 |
OMAi | CQHGPID |
OrthoDBi | 971318at2759 |
PhylomeDBi | G5EGH6 |
Enzyme and pathway databases
UniPathwayi | UPA00199 UPA01038 |
Reactomei | R-CEL-75105, Fatty acyl-CoA biosynthesis |
Miscellaneous databases
PROi | PR:G5EGH6 |
Gene expression databases
Bgeei | WBGene00001399, Expressed in adult organism and 4 other tissues |
Family and domain databases
CDDi | cd03505, Delta9-FADS-like, 1 hit |
InterProi | View protein in InterPro IPR015876, Acyl-CoA_DS |
PANTHERi | PTHR11351, PTHR11351, 1 hit |
PRINTSi | PR00075, FACDDSATRASE |
MobiDBi | Search... |
Entry informationi
Entry namei | FAT7_CAEEL | |
Accessioni | G5EGH6Primary (citable) accession number: G5EGH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 18, 2013 |
Last sequence update: | December 14, 2011 | |
Last modified: | February 23, 2022 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families