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Protein

Protein mes-1

Gene

mes-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

During early embryogenesis, controls asymmetric cell division and the asymmetric localization of P granules of germline precursor P2 and its descendant P3 (PubMed:7555722). Probably upstream of tyrosine kinase src-1, plays a role in endoderm development by controlling spindle orientation during EMS blastomere cell division (PubMed:12110172). Controls EMS spindle orientation probably by promoting lin-5 and gpr-1/2 enrichment at, and let-99 exclusion from the junction between P2 and EMS cells (PubMed:14534135, PubMed:12730122).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei685ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi662 – 670ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase kinase activity Source: GO_Central
  • protein tyrosine kinase activity Source: WormBase

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiG5EBL2

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mes-1Curated
Alternative name(s):
Maternal-effect sterile protein 1Imported
Gene namesi
Name:mes-1Imported
ORF Names:F54F7.5Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF54F7.5 ; CE28237 ; WBGene00003219 ; mes-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 470ExtracellularCuratedAdd BLAST451
Transmembranei471 – 491HelicalSequence analysisAdd BLAST21
Topological domaini492 – 966CytoplasmicCuratedAdd BLAST475

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

71 percent of embryos of mutant mothers develop into sterile adults with extra body muscle cells at the restrictive temperature (25 degrees Celsius) (PubMed:7555722). Low embryonic lethality (7-8 percent) (PubMed:7555722). Loss of lin-5 and grp-1/2 enrichment at the EMS/P2 cell boundaries at the 4-cell embryonic stage (PubMed:12730122). Decrease in phosphotyrosine levels at the site of contact between P2 and EMS cells (PubMed:12110172).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi89G → E in q367; 12 percent of mutant mothers' progeny is sterile at the permissive temperature (16 degrees Celsius) and 79 percent at the restrictive temperature (25 degrees Celsius). Low levels of embryonic lethality. 1 Publication1
Mutagenesisi441S → N in q222; 6 percent of mutant mothers' progeny is sterile at the permissive temperature (16 degrees Celsius) and 77 percent at the restrictive temperature (25 degrees Celsius). Low levels of embryonic lethality. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_500534909720 – 966Protein mes-1Sequence analysisAdd BLAST947

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi126N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi183N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi214N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi251N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi372N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiG5EBL2
PaxDbiG5EBL2

Expressioni

Developmental stagei

Expressed at least in P2 germline cell at 4-cell stage and up to 26-cell stage embryos (PubMed:11003841, PubMed:12110172). Expressed only at L4 stage during larval development and then accumulates in adults (PubMed:11003841).2 Publications

Gene expression databases

BgeeiWBGene00003219 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Interactioni

Protein-protein interaction databases

IntActiG5EBL2, 1 interactor
STRINGi6239.F54F7.5

Structurei

3D structure databases

ProteinModelPortaliG5EBL2
SMRiG5EBL2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini656 – 966Protein kinasePROSITE-ProRule annotationAdd BLAST311

Domaini

The protein kinase domain is predicted to be catalytically inactive.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410JKTY Eukaryota
ENOG411187E LUCA
OrthoDBiEOG093708VD

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00219 TyrKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G5EBL2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIHHFLTLL CTFLPLTTTA LTNSTPLSLL GPCYKRCVTK FGETKEQLTN
60 70 80 90 100
AETISLEYDV SNNTEFSLCK LGCNSHEYTD LNLAAFRYGQ LAYQKILTTV
110 120 130 140 150
EDVPTRGTVL NDVFIVCLDT SFMPSNNSAP SAKRLLSGTV LLVLDEDVAK
160 170 180 190 200
ADNVFLIEVL ARNADKSAVQ VISQQWCYSS NCNITFNAPT EVSSFDVRLR
210 220 230 240 250
VSTFDSNGQV GGINFSKWHN INQILTKTFV DMSLKSVVWK AEKAAANFVF
260 270 280 290 300
NLTASDHVPA CSLQMIYRSS LSSELLHRNF YLDHTLEVFV NNLDFDKIYT
310 320 330 340 350
MQLAPSGTHD RSTPSLASAV IEIPPCRHLV DDYSMCAPPP VSSLSYNWNL
360 370 380 390 400
SPTSEYELLI KWKLLNYMDG LNVTEELSIP VAYFLLNAHP LITANNEQCE
410 420 430 440 450
KYEKIRRVVS YGLRELVFHV PDTDCNYEVE MTAVDTNQRI SEVKKIQVFR
460 470 480 490 500
FNVPPYVSFL QASDIPTSVE LMAVVLATSA IFALIALFLL YRKRKRDKKA
510 520 530 540 550
RFQMYKDAEA GVSYDYVATT ESLGSVVQIR STNFRFEPVE NIDGNIEAAL
560 570 580 590 600
AQQQKFEGGT MNSMFRTYYN LDHPVKVPAH MAEASSDEDN GYENIRYSYF
610 620 630 640 650
GSELSDDVFE EDIYMTHKSL SIYCQDSPLT TPMAPIAPYE HFDDIPSHQY
660 670 680 690 700
RNFQVHNFNE RIEKQAYWLM ATVVDVVRRE LYSLKVPKDY TPETISAMRK
710 720 730 740 750
ELEFLRTLAP HGNCRRFEGV VIGRWDDLPR QVIGILIENT RGGTLRNYIA
760 770 780 790 800
AVGSVFRNCS LATDHDSFAS QQDMNSTQHP FDKLSTEADE NNSKKVKIQE
810 820 830 840 850
ITDSLSIRFC QFAEQVSSAL EHLHSAGSVH TRVTTLNIYL LHNYSDPFDM
860 870 880 890 900
LPDQVVKLGN FGFAVQNSED VVLDDNLQPP EVIKGEKYEA RGDIWQFGLC
910 920 930 940 950
LAEMCSLGDL EQSEVGTLKS GHDTFKNLPS TQVLRDAAKR CLSARTRPSA
960
SDLCGVFKSV NVAATV
Length:966
Mass (Da):108,898
Last modified:December 14, 2011 - v1
Checksum:i07B0000EBA14437F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200199 mRNA Translation: AAF13716.1
BX284606 Genomic DNA Translation: CAA91758.2
PIRiT22681
RefSeqiNP_741906.1, NM_171780.4
UniGeneiCel.6122

Genome annotation databases

EnsemblMetazoaiF54F7.5; F54F7.5; WBGene00003219
GeneIDi181362
KEGGicel:CELE_F54F7.5

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200199 mRNA Translation: AAF13716.1
BX284606 Genomic DNA Translation: CAA91758.2
PIRiT22681
RefSeqiNP_741906.1, NM_171780.4
UniGeneiCel.6122

3D structure databases

ProteinModelPortaliG5EBL2
SMRiG5EBL2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiG5EBL2, 1 interactor
STRINGi6239.F54F7.5

Proteomic databases

EPDiG5EBL2
PaxDbiG5EBL2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF54F7.5; F54F7.5; WBGene00003219
GeneIDi181362
KEGGicel:CELE_F54F7.5

Organism-specific databases

CTDi181362
WormBaseiF54F7.5 ; CE28237 ; WBGene00003219 ; mes-1

Phylogenomic databases

eggNOGiENOG410JKTY Eukaryota
ENOG411187E LUCA
OrthoDBiEOG093708VD

Enzyme and pathway databases

SignaLinkiG5EBL2

Miscellaneous databases

PROiPR:G5EBL2

Gene expression databases

BgeeiWBGene00003219 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00219 TyrKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMES1_CAEEL
AccessioniPrimary (citable) accession number: G5EBL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: December 14, 2011
Last modified: October 10, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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Main funding by: National Institutes of Health

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