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Protein

Alpha-amylase

Gene

ANIA_02018

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Calcium 1UniRule annotation1
Metal bindingi175Calcium 1; via carbonyl oxygenUniRule annotation1
Metal bindingi188Calcium 1UniRule annotation1
Active sitei219NucleophileUniRule annotation1
Metal bindingi219Calcium 2UniRule annotation1
Metal bindingi223Calcium 1; via carbonyl oxygenUniRule annotation1
Active sitei243Proton donorUniRule annotation1
Metal bindingi243Calcium 2UniRule annotation1
Sitei310Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation
LigandCalciumUniRule annotation, Metal-bindingUniRule annotation

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
ORF Names:ANIA_02018Imported
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)Imported
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VII

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
ChainiPRO_501238423716 – 490Alpha-amylaseSequence analysisAdd BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 52UniRule annotation
Disulfide bondi163 ↔ 177UniRule annotation
Disulfide bondi253 ↔ 296UniRule annotation
Disulfide bondi453 ↔ 488UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00008686

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

HOGENOMiHOG000165530
KOiK01176
OMAiEGQTFWK
OrthoDBiEOG092C1HLH

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR013777 A-amylase-like
IPR015340 A_amylase_DUF1966_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF09260 DUF1966, 1 hit
PIRSFiPIRSF001024 Alph-amyl_fung, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

G5EB45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSPLFLSFA ATVLAATPAE WRSQSIYFLL TDRFARTDNS TTAECDTSAK
60 70 80 90 100
YCGGTWQGII NQLDYIQGMG FTAIWITPVT ANLEDGQHGE AYHGYWQQDI
110 120 130 140 150
YALNPHFGTQ DDLRALSDAL HDRGMYLMVD VVANHFGYDA PAASVDYSAF
160 170 180 190 200
NPFNSADYFH TPCDITDYDN QTQVEDCWLY TDAVSLPDVD TTNEEVKEIW
210 220 230 240 250
YDWVGDLVSD YSIDGLRIDT ARHVQKDFWR DYNDAAGVYC VGEVFQGDPD
260 270 280 290 300
YTCGYQEVMD GVLNYPIYYP LLRAFSSTSG SLSDLANMIE TVKYTCSDAT
310 320 330 340 350
LLGNFIENHD NPRFASYTDD ISLAKNVAAF VILSDGIPII YAGQEQHYSG
360 370 380 390 400
AGDPANREAT WLSGYDSTSE LYQFISKTNQ IRNHAIWQNE TYLSYKNYAI
410 420 430 440 450
YNENNVLAMR KGFDGSQIIT ILTNAGADAG SSTVSVPNTG FTAGAAVTEI
460 470 480 490
YTCEDITVSG SGEVSVPMES GLPRVLYPKA KLEGSGICGL
Length:490
Mass (Da):54,250
Last modified:December 14, 2011 - v1
Checksum:iA891C4ACEAEB5305
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BN001307 Genomic DNA Translation: CBF86021.1
RefSeqiXP_659622.1, XM_654530.1

Genome annotation databases

EnsemblFungiiCBF86021; CBF86021; ANIA_02018
EAA64850; EAA64850; AN2018.2
GeneIDi2875021
KEGGiani:AN2018.2

Similar proteinsi

Entry informationi

Entry nameiG5EB45_EMENI
AccessioniPrimary (citable) accession number: G5EB45
Secondary accession number(s): C8VLE6
Entry historyiIntegrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: July 18, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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