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Protein

Thiamine thiazole synthase

Gene

thiA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Substrate; via amide nitrogenUniRule annotation1
Binding sitei115Substrate; via amide nitrogenUniRule annotation1
Binding sitei183Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei237SubstrateUniRule annotation1
Binding sitei289Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:thiAUniRule annotation
Synonyms:thi4, thiF
ORF Names:AN3928
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome II
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004158741 – 331Thiamine thiazole synthaseAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2202,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-220 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Proteomic databases

PRIDEiG5EAZ2

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi162425.CADANIAP00004767

Structurei

3D structure databases

ProteinModelPortaliG5EAZ2
SMRiG5EAZ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 108Substrate bindingUniRule annotation2
Regioni299 – 301Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiG5EAZ2
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

G5EAZ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSPPAAIFEP TVAPTGIKGK VVVPETATIP GDSQTKLLDH FGGKWDNFKF
60 70 80 90 100
APIRESQVSR AMTRRYFQDL DRYAESDVVI VGAGSCGLST AYVLAKARPD
110 120 130 140 150
LKIAIIEASV SPGGGAWLGG QLFSAMVMRR PAELFLNELG VPYEEDPDMP
160 170 180 190 200
NYVVVKHASL FTSTLLSKVL SFPNVKLFNA TCVEDLVTRP GPNGNAQEVQ
210 220 230 240 250
IAGVVTNWTL VTLHHDDHSC MDPNTINAPV IISTTGHDGP FGAFSAKRLV
260 270 280 290 300
SMTTIDKLGG MRGLDMNSAE DAIVKNTREV AKGLIIGGME LSEIDGFNRM
310 320 330
GPTFGAMVLS GVKAAEEALR VFDDRKRECA E
Length:331
Mass (Da):35,616
Last modified:December 14, 2011 - v1
Checksum:i2129CB2DCBD53160
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BN001302 Genomic DNA Translation: CBF75074.1
AACD01000064 Genomic DNA Translation: EAA59237.1
RefSeqiXP_661532.1, XM_656440.1

Genome annotation databases

EnsemblFungiiCBF75074; CBF75074; ANIA_03928
EAA59237; EAA59237; AN3928.2
GeneIDi2873351
KEGGiani:AN3928.2

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BN001302 Genomic DNA Translation: CBF75074.1
AACD01000064 Genomic DNA Translation: EAA59237.1
RefSeqiXP_661532.1, XM_656440.1

3D structure databases

ProteinModelPortaliG5EAZ2
SMRiG5EAZ2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi162425.CADANIAP00004767

Proteomic databases

PRIDEiG5EAZ2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCBF75074; CBF75074; ANIA_03928
EAA59237; EAA59237; AN3928.2
GeneIDi2873351
KEGGiani:AN3928.2

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiG5EAZ2
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_EMENI
AccessioniPrimary (citable) accession number: G5EAZ2
Secondary accession number(s): C8V649
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: December 14, 2011
Last modified: June 20, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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